In the HPA program, the objective has been that all immunohistochemistry images should be manually annotated by a certified pathologist. A web-based annotation tool was developed taking advantage that all tissue images was scanned to generate high resolution digital images. A “annotation jamboree” was organized in which a large number of Scandinavian pathologists came together and performed annotation of hundreds of thousands of pathology images. However, due to the increased throughput of images, a new strategy to keep up with the production of images was necessary. A dedicated site with pathologists was set-up in Mumbai, India headed by Dr Sanjay Navani. This HPA affiliated site have now annotated more than 10 million images published on the open access resource (Human Protein Atlas).

Key publication

• Pontén F et al., The Human Protein Atlas - a tool for pathology. J Pathol. (2008)
  PubMed: 18853439 DOI: 10.1002/path.2440

Other selected publications

• Björling E et al., A web-based tool for in silico biomarker discovery based on tissue-specific protein profiles in normal and cancer tissues. Mol Cell Proteomics. (2008)
  PubMed: 17913849 DOI: 10.1074/mcp.M700411-MCP200

• Pontén F et al., A global view of protein expression in human cells, tissues, and organs. Mol Syst Biol. (2009)
  PubMed: 20029370 DOI: 10.1038/msb.2009.93

• Uhlen M et al., Towards a knowledge-based Human Protein Atlas. Nat Biotechnol. (2010)
  PubMed: 21139605 DOI: 10.1038/nbt1210-1248