Cell lines

Name	Description of cell line	Original reference	Origin	Sex & Age	Source	Cellosaurus (ExPaSy)
Brain
U-87 MG*	Glioblastoma, astrocytoma cell line	Ponten J., Macintyre EH., Acta Pathol. Microbiol. Scand. 74: 465-486 (1968)	Brain	Male, 44	LGC/ATCC	CVCL_0022 Cancer cell line
U-251 MG	Glioblastoma cell line	Westermark B., Int J Cancer12: 438 (1973)	Brain	Male	BW¹	CVCL_0021 Cancer cell line
U-138 MG	Glioblastoma cell line	Ponten et al., Acta Pathol Microbiol Scand 74: 465 (1968)	Brain	Male, 47	DSMZ	CVCL_0020 Cancer cell line
SH-SY5Y	Metastatic neuroblastoma, clonal subline of neuroepithelioma cell line SK-N-SH	Biedler et al., Cancer Res 33: 2643 (1973)	Bone marrow	Female, 4	DSMZ	CVCL_0019 Cancer cell line
GAMG	Glioblastoma cell line	Akslen et al, Anticancer Res. 8(4): 797-803 (1988)		Female, 42	DSMZ	CVCL_1226 Cancer cell line
AF22	iPS derived Neuroepithelia stem (NES) cell line	Falk et al., PLoS One 7(1) (2012)	Skin	Female, 36	NA	Uncategorised
Eye
HTERT-RPE1	Retinal pigment epithelial cells immortalized with hTERT	Rambhatla L et al.. Invest Ophthalmol Vis Sci. 43(5):1622-30. (2002)	Retina, eye	Female, 1		CVCL_4388 Telomerase immortalized cell line
HTCEpi	Telomerase-immortalized corneal epithelial cell line	Robertson et al., Invest Ophthalmol Vis Sci 46(2): 470-478 (2005)	Cornea	Male, 62	Evercyte	CVCL_AQ44 Telomerase immortalized cell line
Proximal digestive tract
OE19	Esophageal adenocarcinoma cell line	PMID: 9010035	Esophagus	Male, 72	DSMZ	CVCL_1622 Cancer cell line
Gastrointestinal tract
CACO-2	Colon adenocarcinoma cell line	Fogh J., et al. J. Natl. Cancer Inst. 58: 209 (1977)	Colon	Male, 72	DSMZ	CVCL_0025 Cancer cell line
Liver & Gallbladder
Hep G2	Hepatocellular carcinoma cell line	Aden et al., Nature 282: 615 (1979)	Liver	Male, 15	DSMZ	CVCL_0027 Cancer cell line
Pancreas
CAPAN-2	Pancreas adenocarcinoma cell line	Kyriazis et al., Cancer Res 46: 5810 (1986)	Pancreas	Male, 56	DSMZ	CVCL_0026 Cancer cell line
Kidney & Urinary bladder
RT4	Urinary bladder transitional cell carcinoma cell line	Rigby et al., Br J Cancer 24: 746 (1970)	Urinary bladder	Male, 63	DSMZ/ECACC	CVCL_0036 Cancer cell line
RPTEC TERT1	Proximal tubular epithelial cell line	Wieser et al., Am J Physiol Renal Physiol 295(5): 1365-1375 (2008)	Human kidney cortex	Male	Evercyte	CVCL_K278 Telomerase immortalized cell line
NTERA-2	Metastatic embryonal carcinoma cell line, cloned from TERA-2	Fogh & Trempe, Human Tumor Cells in Vitro : 115 (1975)		Male, 22	DSMZ	CVCL_0034 Cancer cell line
HEK 293	Embryonal kidney cell line, transformed by adenovirus type 5	Graham et al., J Gen Virol 36: 59 (1977)	Embryonal kidney	Female	DSMZ	CVCL_0045 Transformed cell line
Male reproductive system
SuSa	Testicular germ cell tumor	PMID: 593370	Testis	Male, 30	DSMZ	CVCL_L280 Cancer cell line
PC-3	Metastatic poorly differentiated prostate adenocarcinoma cell line	Kaighn et al., Invest Urol 17: 16-23 (1979)	Bone marrow	Male, 62	DSMZ	CVCL_0035 Cancer cell line
Skin
A-431	Epidermoid carcinoma cell line	Giard et al., J Natl Cancer Inst 51: 1417 (1973)	Skin	Female, 85	DSMZ	CVCL_0037 Cancer cell line
HaCaT	Keratinocyte cell line	Boukamp et al., J Cell Biol Mar 106(3): 761 (1988)	Skin	Male	NEF²	CVCL_0038 Spontaneously immortalized cell line
SK-MEL-30	Metastatic malignant melanoma cell line	Carey et al., Proc Natl Acad Sci USA 73: 3278 (1976)	Subcutis	Male, 67	DSMZ	CVCL_0039 Cancer cell line
WM-115	Malignant melanoma cell line	Westermark B. et al, Proc Natl Acad Sci U S A. Oct; 83(19): 7197 (1986)	Skin	Female, 58	BW¹	CVCL_0040 Cancer cell line
Lung
A549	Lung carcinoma cell line	Giard et al., J Natl Cancer Inst 51: 1417 (1973)	Lung	Male, 58	DSMZ	CVCL_0023 Cancer cell line
HBEC3-KT	Immortalized human bronchial epithelial cell line		Central lung bronchiole	Female, 65	Evercyte	CVCL_X491 Telomerase immortalized cell line
SCLC-21H	Small cell lung carcinoma cell line	Bepler et al., J Cancer Res Clin Oncol 113: 31 (1987)	Pleural effusion	Male, 46	DSMZ	CVCL_0024 Cancer cell line
Female reproductive system
MCF7	Metastatic breast adenocarcinoma cell line	Soule et al., J Natl Cancer Inst 51: 1409 (1973)	Pleural effusion	Female, 69	DSMZ	CVCL_0031 Cancer cell line
HTERT-HME1	Immortalized mammary epithelial cell line		Mammary gland	Female, 53	Evercyte	CVCL_3383 Telomerase immortalized cell line
SiHa	Cervical squamous cell carcinoma cell line, integrated 1-2 copies of HPV16	Friedl F. et al.Proc., Soc. Exp. Biol. Med. 135: 543-545 (1970)	Cervix	Female, 55	LGC/ATCC	CVCL_0032 Cancer cell line
SK-BR-3	Metastatic breast adenocarcinoma cell line	Fogh J, editor. Human tumor cells in vitro. New York: Plenum Press (1975)	Pleural effusion	Female, 43	LGC/ATCC	CVCL_0033 Cancer cell line
T-47d	Invasive ductal carcinoma	Keydar et al., Eur J Cancer. May;15(5):659-70 (1979)	Mammary gland; derived from metastatic site: pleural effusion	Female, 54	ATCC	CVCL_0553 Cancer cell line
EFO-21	Metastatic ovarian serous cystadenocarcinoma cell line	Simon et al., J Natl Cancer Inst 70: 839 (1983)	Ascites	Female, 56	DSMZ	CVCL_0029 Cancer cell line
BEWO	Metastatic choriocarcinoma cell line	Pattillo et al., Cancer Res 28: 1231-1236 (1968)	Brain	Male	DSMZ	CVCL_0044 Cancer cell line
AN3-CA	Metastatic endometrial adenocarcinoma cell line	Dawe et al., J Natl Cancer Inst 33: 441 (1964)	Lymph node	Female, 55	DSMZ	CVCL_0028 Cancer cell line
HeLa	Cervical epithelial adenocarcinoma cell line	Scherer et al., J Exp Med 97: 695 (1953)	Cervix	Female, 31	DSMZ	CVCL_0030 Cancer cell line
Endothelial
HUVEC TERT2	Umbilical vein endothelial cell line	NA	Umbilical vein	Female	Evercyte	CVCL_9Q53 Telomerase immortalized cell line
TIME	Telomerase-immortalized human microvascular endothelial cells (pooled)	Venetsanakos et el., Exp Cell Res., Feb 1;273(1): 21-33 (2002)	Skin	Male	LCW5³	CVCL_0047 Telomerase immortalized cell line
Muscle
HSkMC	Primary skeletal muscle cells		Trapezius and erector spinae muscles		3H Biomedical	Uncategorised
Mesenchymal
BJ hTERT+ SV40 Large T+ RasG12V	Adherent BJ fibroblast cell line immortalized with ectopic expression of hTERT, transformed with SV40 Large-T infection, made metastasizing with oncogenic H-Ras	Bodnar et al., Science 279(5349): 349-352 (1998)	Foreskin	Male	NA	Uncategorised
BJ hTERT+ SV40 Large T+	Adherent BJ fibroblast cell line immortalized with ectopic expression of hTERT, transformed with SV40 Large-T infection	Bodnar et al., Science 279(5349): 349-352 (1998)	Foreskin	Male	NA	Uncategorised
BJ hTERT+	Adherent BJ fibroblast cell line immortalized with ectopic expression of hTERT	Bodnar et al., Science 279(5349): 349-352 (1998)	Foreskin	Male	NA	CVCL_6573 Telomerase immortalized cell line
BJ	Adherent fibroblast cell line	Bodnar AG., et al. Science 279: 349-352 (1998)	Foreskin	Male	LGC/ATCC	CVCL_3653 Finite cell line
ASC TERT1	Adipose tissue-derived mesenchymal stem cell line	Wolbank S. et al., Tissue Eng Part A: Jul;15(7):1843-54 (2009)	Adipose tissue	Female	Evercyte	CVCL_U602 Telomerase immortalized cell line
ASC diff	Differentiated imortalized adipose-derived mesenchymal stem cell line	Wolbank S. et al., Tissue Eng Part A: Jul;15(7):1843-54 (2009)	Adipose tissue	Female	Evercyte	Uncategorised
U-2197	Malignant fibrous histiocytoma cell line	Genberg et al., Am J Pathol 135: 1185 (1989)	Subcutis	Male, 79	DSMZ	CVCL_0043 Cancer cell line
U-2 OS	Osteosarcoma cell line	Ponten J., Saksela E. Int. J. Cancer 2: 434 (1967)	Bone	Female, 15	LGC/ATCC	CVCL_0042 Cancer cell line
RH-30	Metastatic rhabdomyosarcoma cell line	Douglass et al., Cytogenet Cell Genet 45: 148-155 (1987)	Bone marrow	Male, 17	DSMZ	CVCL_0041 Cancer cell line
LHCN-M2	Adherent myoblast cell line	Zhu et al., Aging Cell 6(4): 515-523 (2007)	Satellite cells (from pectoralis major muscle)	Male, 41	Evercyte	CVCL_8890 Telomerase immortalized cell line
HHSteC	Primary hepatic stellate cells		Liver		3H Biomedical	Uncategorised
HBF TERT88	Adherent brain fibroblast cell line	NA	Brain	Female	Evercyte	CVCL_9Q52 Telomerase immortalized cell line
FHDF/TERT166	Immortalized human foreskin fibroblast cell line		Foreskin	Male	Evercyte	CVCL_GZ73 Telomerase immortalized cell line
Lymphoid
MOLT-4	Acute lymphoblastic leukemia (T-ALL) cell line	Minowada et al., J Natl Cancer Inst 49: 891 (1972)	Peripheral blood	Male, 19	DSMZ	CVCL_0013 Cancer cell line
HTEC/SVTERT24-B	Immortalized thymic epithelial cell line		Thymus	Female	Evercyte	CVCL_JM27 Transformed cell line
Daudi	Human Burkitt lymphoma cell line	Klein et al., Cancer Res 28: 1300 (1968)	Orbital biopsy	Male, 16	DSMZ	CVCL_0008 Cancer cell line
HDLM-2	Hodgkin lymphoma cell line	Drexler et al., Leuk Res 10: 487 (1986)	Pleural effusion	Male, 74	DSMZ	CVCL_0009 Cancer cell line
JURKAT	T-cell leukemia cell line		Peripheral blood	Male, 14	DSMZ	CVCL_0065 Cancer cell line
U-698	B-cell lymphoma cell line (lymphoblastic lymphosarcoma)	Nilsson et al., Int J Cancer 13: 808-823 (1974)	Tonsil	Male, 7	DSMZ	CVCL_0017 Cancer cell line
U-266/84	Multiple myeloma cell line (1984, in vitro differentiated)	Hellman et al., Eur J Immunol 18:905-910 (1988)	Peripheral blood	Male, 53	KN⁴	CVCL_0016 Cancer cell line
U-266/70	Multiple myeloma cell line (1970, IL-6-dependent)	Nilsson et al Clin Exp Immunol 7:477-489 (1970)	Peripheral blood	Male, 53	KN⁴	CVCL_0015 Cancer cell line
RPMI-8226	Multiple myeloma cell line	Matsuoka et al., Proc Soc Exp Biol Med 125: 1246 (1967)	Peripheral blood	Male, 61	DSMZ	CVCL_0014 Cancer cell line
REH**	Pre-B cell leukemia cell line (ALL, first relapse)	Rosenfeld et al., Nature 267: 841–3 (1977)	Peripheral blood	Female, 15	DSMZ	CVCL_1650 Cancer cell line
Karpas-707	Multiple myeloma cell line	Karpas et al., Lancet Apr 24;1(8278): 931-3 (1982)	Bone marrow and peripheral blood	Male, 53	KN⁴	CVCL_0010 Cancer cell line
Myeloid
HEL	Erythroleukemia cell line (AML M6 in relapse after treatment for Hodgkin's disease)	Martin et al., Science 216: 1233 (1982)	Peripheral blood	Male, 30	DSMZ	CVCL_0001 Cancer cell line
U-937	Myelomonocytic histiocytic lymphoma cell line	Sundström C, Nilsson K., Int J Cancer 17:565-577 (1976)	Pleural effusion	Male, 37	KN⁴	CVCL_0007 Cancer cell line
THP-1	Acute monocytic leukemia (AML) cell line	Tsuchiya et al., Int J Cancer 26: 171 (1980)	Peripheral blood	Male, 1	DSMZ	CVCL_0006 Cancer cell line
NB-4	Acute promyelocytic leukemia (APL) cell line	Lanotte et al., Blood 77: 1080 (1991)	Bone marrow	Female, 23	DSMZ	CVCL_0005 Cancer cell line
K-562	Chronic myeloid leukemia (CML) cell line	Lozzio et al., J Natl Cancer Inst 50: 535 (1973)	Pleural effusion	Female, 53	DSMZ	CVCL_0004 Cancer cell line
HMC-1	Mastcell leukemia cell line	Butterfield et al., Leukemia Res. 12: 345-355 (1988)	Peripheral blood	Male, 52	KN⁴	CVCL_0003 Cancer cell line
HL-60	Acute promyelocytic leukemia (APL) cell line	Collins et al., Nature 270: 347-349 (1977)	Peripheral blood	Female, 35	DSMZ	CVCL_0002 Cancer cell line
HAP1	Haploid karyotype cell line from Chronic myelogenous leukemia	Carette et al., Nature 477(7364): 340-343 (2011)	Leukemia	Male	Horizon Discovery	CVCL_Y019 Cancer cell line
Mouse
NIH 3T3	Mouse embryonal fibroblast cell line	Aaronson et al., J Cell Physio 72(2): 141-148 (1968)	Embryo		DSMZ	CVCL_0594 Spontaneously immortalized cell line

Notes

Allen M, Bjerke M, Edlund H, Nelander S and Westermark B: Origin of the U87MG glioma cell line: Good news and bad news. Science Translational Medicine 31 Aug 2016: Vol. 8, Issue 354, pp. 354. DOI: 10.1126/scitranslmed.aaf6853

REH was previously listed as KM3. Due to misidentification, KM3 has been deleted from the DSMZ catalogue and the true identity is REH. DSMZ - False leukemia and lymphoma cell lines. Drexler et al., False leukemia-lymphoma cell lines: An update on over 500 cell lines. Leukemia 17: 416-426 (2003)

Source information

Prof. Bengt Westermark, Uppsala University, Uppsala, Sweden
Prof. Norbert E Fusenig, DKFZ, Heidelberg, Germany
Prof. Martin McMahon, UCSF Comprehensive Cancer Center, San Fransisco, USA
Prof. Kenneth Nilsson, Uppsala University, Uppsala, Sweden

Hematopoietic cell lines

Schematic description of hematopoietic differentiation lineages, and cellular origin for hematopoietic cell lines.

Cell lines

Brain

Eye

Proximal digestive tract

Gastrointestinal tract

Liver & Gallbladder

Pancreas

Kidney & Urinary bladder

Male reproductive system

Skin

Lung

Female reproductive system

Endothelial

Muscle

Mesenchymal

Lymphoid

Myeloid

Mouse

Notes

Source information

Hematopoietic cell lines