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Antibodypedia antibody portal

The portal Antibodypedia (www.antibodypedia.com) was launched in 2008 to allow sharing of information regarding validation of antibodies. The database provides a resource of publicly available antibodies to human proteins with accompanying experimental evidence supporting an individual validation score for each antibody in an application-specific manner. The resource now contains information for antibodies corresponding to over 19,000 human protein targets.

Key publication

Other selected publications

  • Cottingham K., Antibodypedia seeks to answer the question: "how good is that antibody?". J Proteome Res. (2008)
    PubMed: 18767878 DOI: 10.1021/pr800626d

  • Jonasson K et al., The 6th HUPO Antibody Initiative (HAI) workshop: sharing data about affinity reagents and other recent developments. September 2009, Toronto, Canada. Proteomics. (2010)
    PubMed: 20514648 DOI: 10.1002/pmic.201090040

  • Alm T et al., A chromosome-centric analysis of antibodies directed toward the human proteome using Antibodypedia. J Proteome Res. (2014)
    PubMed: 24533432 DOI: 10.1021/pr4011525

  • Alm T et al., Introducing the Affinity Binder Knockdown Initiativeâ¿¿A publicâ¿¿private partnership for validation of affinity reagents. EuPA Open Proteom. (2016)
    PubMed: 29900101 DOI: 10.1016/j.euprot.2016.01.002

Figure legend: Antibodypedia constitutes a tool for evidence-based selection of antibodies for research. The portal enables users to (A) search for antibodies targeting specific proteins for selected applications and (B) compare side-by-side experimental evidence for various antibodies to the same target [example showing three anti-epidermal growth factor receptor (EGFR) antibodies].

Key facts

  • Antibodypedia contains information about more than 4 million antibodies
  • The database contains more than 2 million validation experiments
  • More than half (55%) of the antibodies produced are validated for Western blot
  • Other common applications are immunohistochemistry, immunocytochemistry, and flow cytometry