Methods

The Human Protein Atlas consists of 10 sections. In the follwing, explore the Methods Summary of respective section. .

TISSUE
BRAIN
SINGLE CELL TYPE
TISSUE CELL TYPE
PATHOLOGY
IMMUNE CELL
BLOOD PROTEIN
SUBCELLULAR
CELL LINE
METABOLIC

Technologies

Antibodies are used in a variety of research applications and techniques, such as Western Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, and more. The different conditions in each application and the complexity of biological samples ask for great functionality from the antibodies.

The following pages describe some of the most common technologies.


Antibodypedia

Enzyme-linked immunosorbent assay

Flow cytometry

Immunocytochemistry

Immunoelectron microscopy

Immunohistochemistry

Immunoprecipitation

Immunoproteomics

Proximity ligation assay

Western blot

References and Links

About the Nobel Prize in Physiology or Medicine 1901:
The Nobel Prize in Physiology or Medicine 1901. (n.d.). Retrieved February 19, 2014, from
http://www.nobelprize.org/nobel_prizes/medicine/laureates/1901/

A summary of the immune system and the breakthroughs for which the Nobel Prize has been awarded:
http://www.nobelprize.org/nobel_prizes/medicine/immune_responses.html

Relevant Wikipedia pages:
http://en.wikipedia.org/wiki/Antibodies
http://en.wikipedia.org/wiki/Polyclonal_antibodies
http://en.wikipedia.org/wiki/Monoclonal_antibody

Antibodypedia - An open-access database of publicly available antibodies and their usefulness in various applications:
http://www.antibodypedia.com