Single cell type - SPPL2B - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	SPPL2B
Gene descriptionⁱ Full gene name according to HGNC.	Signal peptide peptidase like 2B
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	5

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Intestinal epithelial cells - Unknown function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Gastric mucus-secreting cells, Late spermatids)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Gastric mucus-secreting cells, Late spermatids)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

SPPL2B is part of cluster 53 Intestinal epithelial cells - Unknown function with confidenceⁱ 1
611 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
ARFGAP1	ADP ribosylation factor GTPase activating protein 1	0.6236	53
GAK	Cyclin G associated kinase	0.5386	53
CACFD1	Calcium channel flower domain containing 1	0.5262	53
EXD3	Exonuclease 3'-5' domain containing 3	0.5256	53
CU639417.1	Histone H2B type F-S-like	0.4982	53
OPLAH	5-oxoprolinase, ATP-hydrolysing	0.4960	25
FAM83E	Family with sequence similarity 83 member E	0.4933	27
FAAH	Fatty acid amide hydrolase	0.4927	53
MST1R	Macrophage stimulating 1 receptor	0.4897	53
TRPM5	Transient receptor potential cation channel subfamily M member 5	0.4893	16
ERI3	ERI1 exoribonuclease family member 3	0.4787	25
STARD10	StAR related lipid transfer domain containing 10	0.4787	25
B4GALNT3	Beta-1,4-N-acetyl-galactosaminyltransferase 3	0.4727	25
ZNF653	Zinc finger protein 653	0.4704	25
MICALL2	MICAL like 2	0.4688	53

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	23.6 nTPM
Adipose tissue	23.9 nTPM
Breast	23.4 nTPM

Alveolar cells type 1	3.2 nTPM
Lung	3.2 nTPM

Alveolar cells type 2	17.2 nTPM
Lung	17.2 nTPM

Astrocytes	57.1 nTPM
Brain	57.1 nTPM

B-cells	30.8 nTPM
PBMC	55.7 nTPM
Adipose tissue	41.6 nTPM
Breast	36.0 nTPM
Stomach	34.5 nTPM
Kidney	33.4 nTPM
Colon	31.9 nTPM
Lymph node	27.3 nTPM
Bone marrow	18.0 nTPM
Spleen	16.9 nTPM
Liver	12.9 nTPM

Dendritic cells	18.8 nTPM
PBMC	27.9 nTPM
Breast	17.8 nTPM
Adipose tissue	10.8 nTPM

Endothelial cells	14.4 nTPM
Ovary	34.2 nTPM
Breast	20.2 nTPM
Skeletal muscle	19.5 nTPM
Endometrium	19.3 nTPM
Placenta	16.5 nTPM
Esophagus	15.5 nTPM
Eye	15.4 nTPM
Heart muscle	15.0 nTPM
Lung	12.5 nTPM
Pancreas	11.6 nTPM
Testis	8.3 nTPM
Liver	7.3 nTPM
Prostate	6.7 nTPM
Skin	0.0 nTPM

Fibroblasts	14.2 nTPM
Ovary	28.2 nTPM
Adipose tissue	24.3 nTPM
Breast	21.4 nTPM
Placenta	19.3 nTPM
Skeletal muscle	18.8 nTPM
Esophagus	12.1 nTPM
Stomach	11.9 nTPM
Lung	11.1 nTPM
Heart muscle	5.0 nTPM
Prostate	3.8 nTPM
Skin	0.0 nTPM

Intestinal goblet cells	63.2 nTPM
Colon	79.4 nTPM
Small intestine	57.1 nTPM
Rectum	53.0 nTPM

Macrophages	10.8 nTPM
Breast	19.6 nTPM
Endometrium	18.1 nTPM
Stomach	17.6 nTPM
Ovary	17.0 nTPM
Adipose tissue	12.5 nTPM
Bone marrow	12.5 nTPM
Spleen	11.4 nTPM
Esophagus	11.0 nTPM
Lung	10.9 nTPM
Skeletal muscle	7.7 nTPM
Kidney	7.6 nTPM
Testis	6.4 nTPM
Pancreas	5.1 nTPM
Prostate	4.6 nTPM
PBMC	0.0 nTPM

Paneth cells	49.9 nTPM
Colon	85.7 nTPM
Rectum	33.9 nTPM
Small intestine	30.0 nTPM

Plasma cells	11.5 nTPM
Adipose tissue	19.4 nTPM
Bone marrow	13.2 nTPM
Stomach	12.0 nTPM
Spleen	6.7 nTPM
Esophagus	6.1 nTPM

Respiratory ciliated cells	10.9 nTPM
Lung	21.9 nTPM
Bronchus	0.0 nTPM

Smooth muscle cells	11.7 nTPM
Ovary	21.7 nTPM
Breast	19.6 nTPM
Endometrium	17.8 nTPM
Adipose tissue	17.2 nTPM
Pancreas	14.5 nTPM
Skeletal muscle	13.0 nTPM
Heart muscle	9.5 nTPM
Prostate	9.1 nTPM
Esophagus	6.2 nTPM
Skin	0.0 nTPM
Bronchus	0.0 nTPM

T-cells	11.0 nTPM
PBMC	24.6 nTPM
Colon	17.6 nTPM
Breast	16.8 nTPM
Lung	16.7 nTPM
Endometrium	13.2 nTPM
Lymph node	12.5 nTPM
Stomach	12.5 nTPM
Skeletal muscle	12.2 nTPM
Adipose tissue	11.3 nTPM
Ovary	11.1 nTPM
Bone marrow	9.3 nTPM
Esophagus	6.6 nTPM
Spleen	6.2 nTPM
Liver	6.1 nTPM
Kidney	4.7 nTPM
Prostate	4.7 nTPM
Skin	0.0 nTPM

Basal prostatic cells	6.5 nTPM
Prostate	6.5 nTPM

Cholangiocytes	20.0 nTPM
Liver	20.0 nTPM

Collecting duct cells	25.8 nTPM
Kidney	25.8 nTPM

Cone photoreceptor cells	47.9 nTPM
Eye	47.9 nTPM

Undifferentiated cells	49.8 nTPM
Colon	59.3 nTPM
Rectum	45.9 nTPM
Small intestine	44.2 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC