Single cell type - BTK - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	BTK
Gene descriptionⁱ Full gene name according to HGNC.	Bruton tyrosine kinase
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Cancer-related genes Disease related genes Enzymes FDA approved drug targets Human disease related genes Metabolic proteins Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	4

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	B-cells - Immune response (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (granulocytes, B-cells, monocytes, Kupffer cells, Macrophages, Hofbauer cells, Plasma cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enriched (basophil)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (Daudi, HEL, HMC-1, U-937)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in some
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (granulocytes, B-cells, monocytes, Kupffer cells, Macrophages, Hofbauer cells, Plasma cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

BTK is part of cluster 57 B-cells - Immune response with confidenceⁱ 1
204 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
LAT2	Linker for activation of T cells family member 2	0.7981	57
P2RX1	Purinergic receptor P2X 1	0.7658	60
ALOX5	Arachidonate 5-lipoxygenase	0.7482	57
SYK	Spleen associated tyrosine kinase	0.7108	57
GAPT	GRB2 binding adaptor protein, transmembrane	0.6946	65
SIGLEC5	Sialic acid binding Ig like lectin 5	0.6861	4
PTPN6	Protein tyrosine phosphatase non-receptor type 6	0.6833	57
ARHGAP25	Rho GTPase activating protein 25	0.6785	60
CYSLTR1	Cysteinyl leukotriene receptor 1	0.6760	57
ARL11	ADP ribosylation factor like GTPase 11	0.6638	57
DOK3	Docking protein 3	0.6437	19
CD180	CD180 molecule	0.6396	57
TLR6	Toll like receptor 6	0.6335	57
SIGLEC8	Sialic acid binding Ig like lectin 8	0.6257	60
HPGDS	Hematopoietic prostaglandin D synthase	0.6136	60

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	1.5 nTPM
Breast	2.8 nTPM
Adipose tissue	0.2 nTPM

Alveolar cells type 1	3.2 nTPM
Lung	3.2 nTPM

Alveolar cells type 2	8.9 nTPM
Lung	8.9 nTPM

Astrocytes	0.3 nTPM
Brain	0.3 nTPM

Basal keratinocytes	1.0 nTPM
Skin	1.0 nTPM

B-cells	84.7 nTPM
PBMC	131.3 nTPM
Kidney	109.5 nTPM
Colon	95.8 nTPM
Spleen	81.3 nTPM
Adipose tissue	81.2 nTPM
Liver	80.7 nTPM
Lymph node	77.6 nTPM
Bone marrow	67.9 nTPM
Stomach	64.4 nTPM
Breast	57.5 nTPM

Dendritic cells	30.1 nTPM
PBMC	87.6 nTPM
Adipose tissue	1.5 nTPM
Breast	1.1 nTPM

Endothelial cells	0.8 nTPM
Lung	3.1 nTPM
Breast	2.0 nTPM
Skin	1.9 nTPM
Liver	0.9 nTPM
Heart muscle	0.8 nTPM
Prostate	0.7 nTPM
Skeletal muscle	0.7 nTPM
Endometrium	0.3 nTPM
Eye	0.0 nTPM
Placenta	0.0 nTPM
Testis	0.0 nTPM
Pancreas	0.0 nTPM
Ovary	0.0 nTPM
Esophagus	0.0 nTPM

Fibroblasts	0.9 nTPM
Lung	2.2 nTPM
Esophagus	2.1 nTPM
Placenta	1.9 nTPM
Breast	1.0 nTPM
Prostate	0.9 nTPM
Skin	0.7 nTPM
Skeletal muscle	0.3 nTPM
Heart muscle	0.2 nTPM
Ovary	0.0 nTPM
Adipose tissue	0.0 nTPM
Stomach	0.0 nTPM

Intestinal goblet cells	0.3 nTPM
Colon	0.7 nTPM
Rectum	0.2 nTPM
Small intestine	0.0 nTPM

Macrophages	61.1 nTPM
PBMC	150.1 nTPM
Ovary	80.4 nTPM
Adipose tissue	72.0 nTPM
Lung	69.5 nTPM
Testis	69.2 nTPM
Breast	67.8 nTPM
Spleen	64.3 nTPM
Pancreas	63.8 nTPM
Kidney	59.0 nTPM
Bone marrow	53.7 nTPM
Skeletal muscle	50.4 nTPM
Esophagus	34.6 nTPM
Endometrium	34.5 nTPM
Stomach	29.4 nTPM
Prostate	18.4 nTPM

Paneth cells	0.5 nTPM
Rectum	1.6 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM

Plasma cells	43.8 nTPM
Bone marrow	74.2 nTPM
Spleen	62.4 nTPM
Esophagus	36.9 nTPM
Stomach	31.5 nTPM
Adipose tissue	14.2 nTPM

Respiratory ciliated cells	0.0 nTPM
Lung	0.0 nTPM
Bronchus	0.0 nTPM

Smooth muscle cells	0.5 nTPM
Esophagus	1.5 nTPM
Skin	1.4 nTPM
Breast	0.5 nTPM
Prostate	0.4 nTPM
Endometrium	0.4 nTPM
Adipose tissue	0.4 nTPM
Heart muscle	0.3 nTPM
Pancreas	0.2 nTPM
Skeletal muscle	0.2 nTPM
Ovary	0.1 nTPM
Bronchus	0.0 nTPM

T-cells	5.7 nTPM
Prostate	37.9 nTPM
Lung	31.9 nTPM
Esophagus	13.1 nTPM
Skeletal muscle	2.5 nTPM
Breast	2.5 nTPM
PBMC	2.1 nTPM
Colon	1.0 nTPM
Liver	0.9 nTPM
Skin	0.9 nTPM
Lymph node	0.9 nTPM
Kidney	0.7 nTPM
Bone marrow	0.6 nTPM
Stomach	0.6 nTPM
Endometrium	0.5 nTPM
Spleen	0.5 nTPM
Adipose tissue	0.2 nTPM
Ovary	0.0 nTPM

Undifferentiated cells	0.1 nTPM
Colon	0.4 nTPM
Rectum	0.1 nTPM
Small intestine	0.0 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC