Single cell type - NUB1 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	NUB1
Gene descriptionⁱ Full gene name according to HGNC.	Negative regulator of ubiquitin like proteins 1
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	6

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Monocytes - Immune response regulation (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (monocytes, Early spermatids)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (monocytes, Early spermatids)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

NUB1 is part of cluster 3 Monocytes - Immune response regulation with confidenceⁱ 1
114 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
IL4I1	Interleukin 4 induced 1	0.7885	3
CCL22	C-C motif chemokine ligand 22	0.7553	3
CCL17	C-C motif chemokine ligand 17	0.7529	3
CRLF2	Cytokine receptor like factor 2	0.7455	3
FOXD4L1	Forkhead box D4 like 1	0.7357	3
IL12B	Interleukin 12B	0.7318	3
IDO1	Indoleamine 2,3-dioxygenase 1	0.7128	3
NCCRP1	NCCRP1, F-box associated domain containing	0.7068	3
CD80	CD80 molecule	0.6997	3
DHX58	DExH-box helicase 58	0.6828	3
CCR7	C-C motif chemokine receptor 7	0.6695	3
ZBTB17	Zinc finger and BTB domain containing 17	0.6645	3
IL2RA	Interleukin 2 receptor subunit alpha	0.6595	3
FOXD4L3	Forkhead box D4 like 3	0.6552	3
CD70	CD70 molecule	0.6538	3

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	61.0 nTPM
Adipose tissue	69.9 nTPM
Breast	52.1 nTPM

Alveolar cells type 1	73.7 nTPM
Lung	73.7 nTPM

Alveolar cells type 2	66.4 nTPM
Lung	66.4 nTPM

Astrocytes	14.0 nTPM
Brain	14.0 nTPM

B-cells	52.7 nTPM
Adipose tissue	74.7 nTPM
Kidney	57.1 nTPM
PBMC	57.1 nTPM
Lymph node	55.7 nTPM
Bone marrow	54.8 nTPM
Stomach	54.5 nTPM
Breast	50.9 nTPM
Colon	49.5 nTPM
Spleen	47.1 nTPM
Liver	25.3 nTPM

Dendritic cells	91.8 nTPM
Adipose tissue	130.6 nTPM
PBMC	79.7 nTPM
Breast	65.2 nTPM

Endothelial cells	45.8 nTPM
Eye	72.0 nTPM
Lung	65.5 nTPM
Esophagus	52.6 nTPM
Liver	51.3 nTPM
Heart muscle	50.6 nTPM
Skeletal muscle	49.1 nTPM
Pancreas	42.5 nTPM
Endometrium	40.5 nTPM
Breast	40.5 nTPM
Testis	39.1 nTPM
Placenta	38.7 nTPM
Ovary	35.5 nTPM
Prostate	34.6 nTPM
Skin	29.1 nTPM

Fibroblasts	42.3 nTPM
Adipose tissue	71.1 nTPM
Lung	56.9 nTPM
Stomach	46.0 nTPM
Heart muscle	40.7 nTPM
Skeletal muscle	40.4 nTPM
Esophagus	39.0 nTPM
Breast	38.6 nTPM
Prostate	37.3 nTPM
Ovary	33.3 nTPM
Placenta	30.9 nTPM
Skin	30.7 nTPM

Intestinal goblet cells	40.9 nTPM
Colon	52.1 nTPM
Rectum	40.0 nTPM
Small intestine	30.4 nTPM

Macrophages	46.7 nTPM
Lung	113.3 nTPM
Endometrium	72.1 nTPM
Breast	66.4 nTPM
Adipose tissue	61.3 nTPM
Ovary	52.4 nTPM
Kidney	52.2 nTPM
Bone marrow	46.2 nTPM
Pancreas	46.0 nTPM
Spleen	43.5 nTPM
Testis	41.8 nTPM
Esophagus	39.3 nTPM
Skeletal muscle	31.7 nTPM
Stomach	17.6 nTPM
Prostate	16.0 nTPM
PBMC	0.0 nTPM

Paneth cells	20.5 nTPM
Rectum	26.2 nTPM
Small intestine	18.8 nTPM
Colon	16.5 nTPM

Plasma cells	22.3 nTPM
Adipose tissue	58.0 nTPM
Stomach	20.3 nTPM
Spleen	14.1 nTPM
Esophagus	12.3 nTPM
Bone marrow	6.6 nTPM

Respiratory ciliated cells	55.7 nTPM
Lung	78.7 nTPM
Bronchus	32.7 nTPM

Smooth muscle cells	43.3 nTPM
Heart muscle	103.3 nTPM
Adipose tissue	51.9 nTPM
Pancreas	48.9 nTPM
Breast	46.5 nTPM
Prostate	43.5 nTPM
Endometrium	40.2 nTPM
Esophagus	38.8 nTPM
Skeletal muscle	36.3 nTPM
Ovary	36.3 nTPM
Skin	21.3 nTPM
Bronchus	9.5 nTPM

T-cells	60.0 nTPM
Adipose tissue	129.8 nTPM
Lung	92.7 nTPM
Ovary	86.7 nTPM
PBMC	71.5 nTPM
Colon	63.7 nTPM
Endometrium	61.6 nTPM
Kidney	59.6 nTPM
Lymph node	58.6 nTPM
Breast	58.3 nTPM
Skeletal muscle	57.9 nTPM
Spleen	54.8 nTPM
Liver	54.5 nTPM
Stomach	54.1 nTPM
Bone marrow	51.3 nTPM
Skin	26.7 nTPM
Esophagus	19.7 nTPM
Prostate	19.0 nTPM

Basal keratinocytes	24.9 nTPM
Skin	24.9 nTPM

Basal prostatic cells	33.8 nTPM
Prostate	33.8 nTPM

Basal respiratory cells	36.4 nTPM
Bronchus	36.4 nTPM

Basal squamous epithelial cells	39.9 nTPM
Esophagus	39.9 nTPM

Undifferentiated cells	26.0 nTPM
Colon	31.6 nTPM
Rectum	24.4 nTPM
Small intestine	22.0 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC