Single cell type - WAS - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	WAS
Gene descriptionⁱ Full gene name according to HGNC.	WASP actin nucleation promoting factor
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Cancer-related genes Disease related genes Human disease related genes
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	2

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Monocytes & Neutrophils - Innate immune response (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Macrophages, Hofbauer cells, Kupffer cells, T-cells, dendritic cells, monocytes, NK-cells, B-cells, Plasma cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enhanced (eosinophil)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (Daudi, HEL, HMC-1, MOLT-4, NB-4, RPMI-8226, THP-1, U-266/70, U-937)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in some
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Macrophages, Hofbauer cells, Kupffer cells, T-cells, dendritic cells, monocytes, NK-cells, B-cells, Plasma cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

WAS is part of cluster 19 Monocytes & Neutrophils - Innate immune response with confidenceⁱ 1
175 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
VAV1	Vav guanine nucleotide exchange factor 1	0.7616	57
PLCB2	Phospholipase C beta 2	0.7556	19
LCP2	Lymphocyte cytosolic protein 2	0.7383	58
HCLS1	Hematopoietic cell-specific Lyn substrate 1	0.7286	63
SNX20	Sorting nexin 20	0.6791	63
CSK	C-terminal Src kinase	0.6739	57
FGR	FGR proto-oncogene, Src family tyrosine kinase	0.6642	19
LCP1	Lymphocyte cytosolic protein 1	0.6597	19
DOCK8	Dedicator of cytokinesis 8	0.6578	19
AIF1	Allograft inflammatory factor 1	0.6564	19
CASP1	Caspase 1	0.6458	19
LST1	Leukocyte specific transcript 1	0.6358	19
COTL1	Coactosin like F-actin binding protein 1	0.6349	19
CD101	CD101 molecule	0.6308	19
RIN3	Ras and Rab interactor 3	0.6295	63

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	1.5 nTPM
Breast	2.8 nTPM
Adipose tissue	0.3 nTPM

Alveolar cells type 1	3.2 nTPM
Lung	3.2 nTPM

Alveolar cells type 2	14.3 nTPM
Lung	14.3 nTPM

Astrocytes	0.7 nTPM
Brain	0.7 nTPM

Basal keratinocytes	2.4 nTPM
Skin	2.4 nTPM

B-cells	83.2 nTPM
Stomach	108.6 nTPM
Spleen	107.3 nTPM
Bone marrow	104.6 nTPM
Lymph node	104.4 nTPM
Kidney	85.7 nTPM
Colon	68.7 nTPM
Adipose tissue	66.7 nTPM
PBMC	65.7 nTPM
Liver	65.1 nTPM
Breast	54.9 nTPM

Dendritic cells	91.7 nTPM
Adipose tissue	115.3 nTPM
Breast	103.9 nTPM
PBMC	55.8 nTPM

Endothelial cells	2.6 nTPM
Eye	17.0 nTPM
Lung	6.2 nTPM
Liver	3.7 nTPM
Esophagus	3.6 nTPM
Placenta	1.7 nTPM
Skin	1.6 nTPM
Breast	1.0 nTPM
Skeletal muscle	0.9 nTPM
Endometrium	0.6 nTPM
Heart muscle	0.5 nTPM
Prostate	0.1 nTPM
Testis	0.0 nTPM
Pancreas	0.0 nTPM
Ovary	0.0 nTPM

Fibroblasts	1.5 nTPM
Stomach	4.4 nTPM
Placenta	3.4 nTPM
Lung	2.2 nTPM
Breast	2.1 nTPM
Skin	1.5 nTPM
Esophagus	1.3 nTPM
Skeletal muscle	0.4 nTPM
Prostate	0.2 nTPM
Adipose tissue	0.2 nTPM
Heart muscle	0.1 nTPM
Ovary	0.1 nTPM

Intestinal goblet cells	0.2 nTPM
Small intestine	0.4 nTPM
Rectum	0.2 nTPM
Colon	0.0 nTPM

Macrophages	118.1 nTPM
Stomach	216.8 nTPM
Bone marrow	181.2 nTPM
Adipose tissue	169.5 nTPM
Kidney	161.1 nTPM
Spleen	160.7 nTPM
Skeletal muscle	118.7 nTPM
Testis	117.3 nTPM
Esophagus	108.6 nTPM
Breast	106.7 nTPM
Endometrium	90.5 nTPM
Lung	81.3 nTPM
Pancreas	79.2 nTPM
Prostate	73.3 nTPM
Ovary	65.3 nTPM
PBMC	40.9 nTPM

Paneth cells	0.1 nTPM
Rectum	0.4 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM

Plasma cells	57.8 nTPM
Spleen	94.4 nTPM
Bone marrow	84.1 nTPM
Stomach	54.9 nTPM
Adipose tissue	37.4 nTPM
Esophagus	18.4 nTPM

Respiratory ciliated cells	1.6 nTPM
Lung	2.9 nTPM
Bronchus	0.2 nTPM

Smooth muscle cells	1.3 nTPM
Skeletal muscle	5.8 nTPM
Breast	3.6 nTPM
Esophagus	1.5 nTPM
Skin	1.0 nTPM
Adipose tissue	0.7 nTPM
Pancreas	0.6 nTPM
Heart muscle	0.4 nTPM
Endometrium	0.4 nTPM
Ovary	0.3 nTPM
Prostate	0.0 nTPM
Bronchus	0.0 nTPM

Distal tubular cells	1.9 nTPM
Kidney	1.9 nTPM

T-cells	99.3 nTPM
Stomach	148.8 nTPM
Bone marrow	136.6 nTPM
Spleen	136.5 nTPM
Esophagus	117.9 nTPM
Kidney	116.3 nTPM
Liver	115.2 nTPM
Lymph node	109.4 nTPM
Adipose tissue	102.1 nTPM
Colon	94.6 nTPM
Breast	94.3 nTPM
PBMC	90.0 nTPM
Ovary	88.9 nTPM
Lung	88.1 nTPM
Skin	74.6 nTPM
Skeletal muscle	74.4 nTPM
Endometrium	57.2 nTPM
Prostate	42.6 nTPM

Undifferentiated cells	0.1 nTPM
Rectum	0.4 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC