Single cell type - ADAM28 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ADAM28
Gene descriptionⁱ Full gene name according to HGNC.	ADAM metallopeptidase domain 28
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Plasma proteins Transporters
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Membrane,Secreted
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	4

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	B-cells - Immune response (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (B-cells, Microglial cells, Gastric mucus-secreting cells, Club cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enhanced (naive B-cell, memory B-cell)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Group enriched (CAPAN-2, OE19)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in some
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level
Extracellular locationⁱ A possible secretion of the protein was predicted based on N-terminal signal sequence (signal peptide) predictions and transmembrane region predictions, and subsequently annotated for its local or systemic secretion based on literature, function, and protein and RNA expression data. Read more	Protein predicted to be secreted

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (B-cells, Microglial cells, Gastric mucus-secreting cells, Club cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

ADAM28 is part of cluster 57 B-cells - Immune response with confidenceⁱ 1
204 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
CD72	CD72 molecule	0.9099	57
FCER2	Fc fragment of IgE receptor II	0.8870	57
TLR10	Toll like receptor 10	0.8813	57
BANK1	B cell scaffold protein with ankyrin repeats 1	0.8773	57
FCRL1	Fc receptor like 1	0.8581	57
MS4A1	Membrane spanning 4-domains A1	0.8568	57
VPREB3	V-set pre-B cell surrogate light chain 3	0.8489	57
PAX5	Paired box 5	0.8468	57
CR2	Complement C3d receptor 2	0.8438	57
CD19	CD19 molecule	0.8402	57
LCN8	Lipocalin 8	0.8352	57
CD37	CD37 molecule	0.8302	57
FCRL2	Fc receptor like 2	0.8258	57
BLK	BLK proto-oncogene, Src family tyrosine kinase	0.8210	57
FCRLA	Fc receptor like A	0.8209	57

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	1.5 nTPM
Adipose tissue	1.9 nTPM
Breast	1.1 nTPM

Alveolar cells type 1	3.2 nTPM
Lung	3.2 nTPM

Alveolar cells type 2	6.0 nTPM
Lung	6.0 nTPM

Astrocytes	0.7 nTPM
Brain	0.7 nTPM

Basal keratinocytes	0.9 nTPM
Skin	0.9 nTPM

B-cells	240.9 nTPM
Stomach	515.9 nTPM
Spleen	306.6 nTPM
Bone marrow	268.2 nTPM
PBMC	259.8 nTPM
Lymph node	243.2 nTPM
Kidney	238.1 nTPM
Adipose tissue	231.6 nTPM
Colon	217.3 nTPM
Breast	93.5 nTPM
Liver	34.3 nTPM

Dendritic cells	9.9 nTPM
PBMC	16.0 nTPM
Adipose tissue	9.9 nTPM
Breast	4.0 nTPM

Endothelial cells	3.7 nTPM
Eye	32.4 nTPM
Lung	6.2 nTPM
Pancreas	3.9 nTPM
Prostate	2.2 nTPM
Testis	1.8 nTPM
Esophagus	1.6 nTPM
Breast	1.0 nTPM
Skin	0.7 nTPM
Heart muscle	0.6 nTPM
Endometrium	0.5 nTPM
Skeletal muscle	0.4 nTPM
Ovary	0.2 nTPM
Liver	0.0 nTPM
Placenta	0.0 nTPM

Fibroblasts	3.9 nTPM
Placenta	15.3 nTPM
Stomach	13.4 nTPM
Ovary	4.7 nTPM
Heart muscle	3.0 nTPM
Breast	1.4 nTPM
Esophagus	1.3 nTPM
Lung	1.2 nTPM
Prostate	1.2 nTPM
Skin	0.7 nTPM
Skeletal muscle	0.2 nTPM
Adipose tissue	0.1 nTPM

Intestinal goblet cells	0.4 nTPM
Small intestine	0.8 nTPM
Rectum	0.4 nTPM
Colon	0.0 nTPM

Macrophages	46.4 nTPM
Testis	120.8 nTPM
Prostate	92.8 nTPM
Breast	91.3 nTPM
Esophagus	81.8 nTPM
Adipose tissue	78.4 nTPM
Endometrium	51.5 nTPM
Stomach	43.9 nTPM
Skeletal muscle	42.6 nTPM
Pancreas	28.0 nTPM
Lung	18.8 nTPM
Kidney	14.3 nTPM
Ovary	12.7 nTPM
Bone marrow	10.0 nTPM
Spleen	8.3 nTPM
PBMC	0.0 nTPM

Paneth cells	0.1 nTPM
Rectum	0.2 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM

Plasma cells	43.3 nTPM
Stomach	94.9 nTPM
Esophagus	67.8 nTPM
Adipose tissue	26.4 nTPM
Spleen	16.0 nTPM
Bone marrow	11.5 nTPM

Respiratory ciliated cells	13.1 nTPM
Lung	19.0 nTPM
Bronchus	7.3 nTPM

Smooth muscle cells	1.1 nTPM
Bronchus	4.8 nTPM
Esophagus	4.6 nTPM
Endometrium	0.9 nTPM
Heart muscle	0.6 nTPM
Breast	0.5 nTPM
Prostate	0.4 nTPM
Skin	0.4 nTPM
Ovary	0.2 nTPM
Pancreas	0.1 nTPM
Skeletal muscle	0.0 nTPM
Adipose tissue	0.0 nTPM

Cholangiocytes	28.6 nTPM
Liver	28.6 nTPM

Excitatory neurons	4.3 nTPM
Brain	4.3 nTPM

Exocrine glandular cells	3.5 nTPM
Pancreas	3.5 nTPM

T-cells	9.0 nTPM
Lung	27.4 nTPM
Esophagus	26.2 nTPM
Colon	25.6 nTPM
Stomach	22.1 nTPM
Prostate	14.2 nTPM
Endometrium	12.1 nTPM
Liver	9.6 nTPM
Skeletal muscle	3.0 nTPM
Kidney	2.7 nTPM
Breast	2.3 nTPM
Lymph node	1.8 nTPM
PBMC	1.7 nTPM
Skin	1.4 nTPM
Spleen	1.3 nTPM
Bone marrow	1.2 nTPM
Adipose tissue	0.8 nTPM
Ovary	0.0 nTPM

Undifferentiated cells	1.3 nTPM
Small intestine	3.1 nTPM
Colon	0.5 nTPM
Rectum	0.4 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC