Single cell type - NEDD4L - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	NEDD4L
Gene descriptionⁱ Full gene name according to HGNC.	NEDD4 like E3 ubiquitin protein ligase
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Cancer-related genes Disease related genes Enzymes Human disease related genes Metabolic proteins Potential drug targets
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	31

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Neurons & Oligodendrocytes - Neuronal signaling (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Excitatory neurons, Alveolar cells type 1)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enriched (T-reg)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in some
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (SK-MEL-30)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Excitatory neurons, Alveolar cells type 1)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

NEDD4L is part of cluster 21 Neurons & Oligodendrocytes - Neuronal signaling with confidenceⁱ 1
589 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
CHRM1	Cholinergic receptor muscarinic 1	0.6203	8
LINGO2	Leucine rich repeat and Ig domain containing 2	0.6171	21
MAGI3	Membrane associated guanylate kinase, WW and PDZ domain containing 3	0.5900	21
PRKCI	Protein kinase C iota	0.5868	44
SP8	Sp8 transcription factor	0.5622	8
CHRM3	Cholinergic receptor muscarinic 3	0.5590	21
KIAA1549L	KIAA1549 like	0.5551	21
ADRA1B	Adrenoceptor alpha 1B	0.5533	21
CACNA1E	Calcium voltage-gated channel subunit alpha1 E	0.5529	21
CACNA1B	Calcium voltage-gated channel subunit alpha1 B	0.5503	21
SLC8A2	Solute carrier family 8 member A2	0.5501	21
CCK	Cholecystokinin	0.5444	8
PROKR2	Prokineticin receptor 2	0.5405	21
MRAP2	Melanocortin 2 receptor accessory protein 2	0.5381	21
MYO5C	Myosin VC	0.5374	11

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	6.0 nTPM
Breast	7.6 nTPM
Adipose tissue	4.4 nTPM

Alveolar cells type 2	144.8 nTPM
Lung	144.8 nTPM

Astrocytes	110.4 nTPM
Brain	110.4 nTPM

B-cells	5.1 nTPM
Breast	12.1 nTPM
Colon	6.4 nTPM
Adipose tissue	6.0 nTPM
Stomach	5.9 nTPM
Kidney	4.8 nTPM
PBMC	4.3 nTPM
Lymph node	3.8 nTPM
Liver	3.5 nTPM
Bone marrow	2.5 nTPM
Spleen	2.1 nTPM

Basal keratinocytes	112.8 nTPM
Skin	112.8 nTPM

Dendritic cells	3.6 nTPM
Breast	6.9 nTPM
Adipose tissue	3.9 nTPM
PBMC	0.0 nTPM

Endothelial cells	14.7 nTPM
Liver	55.9 nTPM
Prostate	25.0 nTPM
Lung	21.8 nTPM
Breast	15.1 nTPM
Endometrium	13.5 nTPM
Ovary	13.2 nTPM
Eye	9.7 nTPM
Heart muscle	9.7 nTPM
Skin	9.3 nTPM
Esophagus	7.8 nTPM
Pancreas	7.7 nTPM
Placenta	6.8 nTPM
Skeletal muscle	6.4 nTPM
Testis	3.8 nTPM

Fibroblasts	9.5 nTPM
Lung	29.0 nTPM
Prostate	18.8 nTPM
Heart muscle	14.6 nTPM
Placenta	13.9 nTPM
Ovary	9.0 nTPM
Skin	5.9 nTPM
Breast	4.6 nTPM
Stomach	4.4 nTPM
Esophagus	1.5 nTPM
Skeletal muscle	1.4 nTPM
Adipose tissue	1.3 nTPM

Intestinal goblet cells	201.5 nTPM
Rectum	239.9 nTPM
Colon	238.7 nTPM
Small intestine	125.9 nTPM

Macrophages	13.3 nTPM
Prostate	60.8 nTPM
Endometrium	19.1 nTPM
Kidney	15.2 nTPM
Breast	15.2 nTPM
Adipose tissue	14.2 nTPM
Lung	13.2 nTPM
Pancreas	12.8 nTPM
Stomach	11.7 nTPM
Ovary	9.1 nTPM
Skeletal muscle	8.5 nTPM
Testis	5.2 nTPM
Spleen	5.2 nTPM
Bone marrow	5.0 nTPM
Esophagus	4.8 nTPM
PBMC	0.0 nTPM

Paneth cells	145.3 nTPM
Colon	283.3 nTPM
Rectum	85.1 nTPM
Small intestine	67.5 nTPM

Plasma cells	12.7 nTPM
Esophagus	30.7 nTPM
Stomach	16.2 nTPM
Bone marrow	6.6 nTPM
Adipose tissue	5.8 nTPM
Spleen	4.2 nTPM

Respiratory ciliated cells	72.7 nTPM
Lung	100.6 nTPM
Bronchus	44.8 nTPM

Smooth muscle cells	6.0 nTPM
Bronchus	18.1 nTPM
Prostate	15.7 nTPM
Ovary	8.5 nTPM
Skin	6.3 nTPM
Heart muscle	5.8 nTPM
Endometrium	5.3 nTPM
Breast	2.7 nTPM
Pancreas	1.8 nTPM
Skeletal muscle	1.0 nTPM
Adipose tissue	0.4 nTPM
Esophagus	0.0 nTPM

T-cells	6.6 nTPM
Prostate	42.6 nTPM
Lung	21.3 nTPM
Kidney	8.9 nTPM
Endometrium	7.9 nTPM
Colon	6.2 nTPM
Adipose tissue	5.8 nTPM
Breast	5.7 nTPM
Skin	5.0 nTPM
Liver	3.5 nTPM
Skeletal muscle	1.8 nTPM
PBMC	1.1 nTPM
Lymph node	0.9 nTPM
Bone marrow	0.8 nTPM
Stomach	0.8 nTPM
Spleen	0.5 nTPM
Ovary	0.0 nTPM
Esophagus	0.0 nTPM

Cholangiocytes	64.3 nTPM
Liver	64.3 nTPM

Collecting duct cells	62.5 nTPM
Kidney	62.5 nTPM

Cone photoreceptor cells	173.0 nTPM
Eye	173.0 nTPM

Cytotrophoblasts	13.0 nTPM
Placenta	13.0 nTPM

Undifferentiated cells	72.9 nTPM
Colon	86.5 nTPM
Rectum	76.7 nTPM
Small intestine	55.4 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC