Single cell type - CALCRL - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	CALCRL
Gene descriptionⁱ Full gene name according to HGNC.	Calcitonin receptor like receptor
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes FDA approved drug targets G-protein coupled receptors Human disease related genes Plasma proteins Transporters
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	5

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Adipocytes & Endothelial cells - Angiogenesis (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Oligodendrocyte precursor cells, Hepatic stellate cells, Endothelial cells, Adipocytes, Endometrial stromal cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Group enriched (myeloid DC, plasmacytoid DC, classical monocyte)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in some
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enriched (HUVEC TERT2)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in some
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Oligodendrocyte precursor cells, Hepatic stellate cells, Endothelial cells, Adipocytes, Endometrial stromal cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

CALCRL is part of cluster 14 Adipocytes & Endothelial cells - Angiogenesis with confidenceⁱ 1
354 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
RHOJ	Ras homolog family member J	0.7765	14
CCL14	C-C motif chemokine ligand 14	0.7740	14
IL33	Interleukin 33	0.7591	14
BMX	BMX non-receptor tyrosine kinase	0.7533	14
ERG	ETS transcription factor ERG	0.7515	14
LRRC70	Leucine rich repeat containing 70	0.7481	14
RAMP3	Receptor activity modifying protein 3	0.7429	14
FLT4	Fms related receptor tyrosine kinase 4	0.7227	14
TIE1	Tyrosine kinase with immunoglobulin like and EGF like domains 1	0.7223	14
CCL23	C-C motif chemokine ligand 23	0.7141	14
TMEM100	Transmembrane protein 100	0.7098	14
MYCT1	MYC target 1	0.7091	14
PLSCR4	Phospholipid scramblase 4	0.7050	14
TEK	TEK receptor tyrosine kinase	0.6925	14
ACKR1	Atypical chemokine receptor 1 (Duffy blood group)	0.6864	14

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Alveolar cells type 1	0.0 nTPM
Lung	0.0 nTPM

Alveolar cells type 2	11.9 nTPM
Lung	11.9 nTPM

Astrocytes	19.6 nTPM
Brain	19.6 nTPM

B-cells	1.2 nTPM
Liver	3.7 nTPM
Spleen	2.1 nTPM
Colon	1.6 nTPM
Lymph node	1.6 nTPM
Breast	1.3 nTPM
Bone marrow	0.8 nTPM
Stomach	0.5 nTPM
Kidney	0.0 nTPM
PBMC	0.0 nTPM
Adipose tissue	0.0 nTPM

Basal keratinocytes	9.3 nTPM
Skin	9.3 nTPM

Adipocytes	138.3 nTPM
Breast	144.8 nTPM
Adipose tissue	131.8 nTPM

Dendritic cells	2.4 nTPM
Adipose tissue	4.7 nTPM
Breast	2.5 nTPM
PBMC	0.0 nTPM

Endothelial cells	248.7 nTPM
Esophagus	633.5 nTPM
Endometrium	446.1 nTPM
Prostate	424.7 nTPM
Liver	374.2 nTPM
Testis	263.7 nTPM
Lung	237.0 nTPM
Placenta	197.2 nTPM
Ovary	176.2 nTPM
Skin	172.3 nTPM
Heart muscle	157.4 nTPM
Skeletal muscle	141.2 nTPM
Pancreas	139.0 nTPM
Breast	94.2 nTPM
Eye	25.1 nTPM

Fibroblasts	56.5 nTPM
Stomach	242.0 nTPM
Esophagus	169.9 nTPM
Heart muscle	59.0 nTPM
Skeletal muscle	57.5 nTPM
Breast	28.1 nTPM
Lung	24.6 nTPM
Adipose tissue	15.9 nTPM
Skin	10.7 nTPM
Ovary	7.1 nTPM
Prostate	6.2 nTPM
Placenta	0.6 nTPM

Intestinal goblet cells	0.0 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM
Rectum	0.0 nTPM

Macrophages	15.4 nTPM
Esophagus	47.2 nTPM
Adipose tissue	42.3 nTPM
Spleen	30.0 nTPM
Endometrium	26.0 nTPM
Skeletal muscle	17.5 nTPM
Ovary	12.9 nTPM
Stomach	11.7 nTPM
Prostate	9.1 nTPM
Kidney	8.3 nTPM
Bone marrow	7.5 nTPM
Lung	6.7 nTPM
Testis	5.2 nTPM
Breast	3.9 nTPM
Pancreas	2.5 nTPM
PBMC	0.0 nTPM

Paneth cells	0.0 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM
Rectum	0.0 nTPM

Plasma cells	4.9 nTPM
Esophagus	18.4 nTPM
Stomach	5.2 nTPM
Adipose tissue	0.6 nTPM
Spleen	0.3 nTPM
Bone marrow	0.0 nTPM

Respiratory ciliated cells	2.8 nTPM
Lung	2.9 nTPM
Bronchus	2.7 nTPM

Smooth muscle cells	21.5 nTPM
Endometrium	45.2 nTPM
Adipose tissue	43.9 nTPM
Breast	40.3 nTPM
Heart muscle	30.2 nTPM
Skeletal muscle	25.5 nTPM
Esophagus	21.7 nTPM
Skin	11.8 nTPM
Prostate	7.5 nTPM
Ovary	6.4 nTPM
Bronchus	2.8 nTPM
Pancreas	0.7 nTPM

Cytotrophoblasts	0.4 nTPM
Placenta	0.4 nTPM

Exocrine glandular cells	0.4 nTPM
Pancreas	0.4 nTPM

T-cells	3.0 nTPM
Esophagus	19.7 nTPM
Endometrium	6.3 nTPM
Lung	6.1 nTPM
Skin	5.5 nTPM
Kidney	4.2 nTPM
Liver	3.5 nTPM
Lymph node	1.5 nTPM
Skeletal muscle	1.3 nTPM
PBMC	0.9 nTPM
Bone marrow	0.6 nTPM
Breast	0.6 nTPM
Spleen	0.5 nTPM
Stomach	0.4 nTPM
Adipose tissue	0.2 nTPM
Colon	0.0 nTPM
Prostate	0.0 nTPM
Ovary	0.0 nTPM

Undifferentiated cells	0.0 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM
Rectum	0.0 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC