Single cell type - MYLK - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	MYLK
Gene descriptionⁱ Full gene name according to HGNC.	Myosin light chain kinase
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Enzymes Human disease related genes Metabolic proteins Plasma proteins Potential drug targets
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	7

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Smooth muscle cells - ECM organization (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Breast myoepithelial cells, Peritubular cells, Smooth muscle cells, Sertoli cells, Hepatocytes)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enhanced (basophil)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in some
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (BJ, BJ hTERT+, fHDF/TERT166, hTERT-RPE1, RPTEC TERT1)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Breast myoepithelial cells, Peritubular cells, Smooth muscle cells, Sertoli cells, Hepatocytes)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

MYLK is part of cluster 48 Smooth muscle cells - ECM organization with confidenceⁱ 1
270 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
RERGL	RERG like	0.8355	48
MYH11	Myosin heavy chain 11	0.8343	48
REM1	RRAD and GEM like GTPase 1	0.8107	48
GPR20	G protein-coupled receptor 20	0.8105	48
MYL9	Myosin light chain 9	0.8044	48
GJA4	Gap junction protein alpha 4	0.7917	48
AVPR1A	Arginine vasopressin receptor 1A	0.7866	48
KCNJ8	Potassium inwardly rectifying channel subfamily J member 8	0.7843	48
ADRA2A	Adrenoceptor alpha 2A	0.7774	48
LMOD1	Leiomodin 1	0.7759	48
FOXC2	Forkhead box C2	0.7689	48
COX4I2	Cytochrome c oxidase subunit 4I2	0.7640	48
NOTCH3	Notch receptor 3	0.7602	48
NRIP2	Nuclear receptor interacting protein 2	0.7602	48
AOC3	Amine oxidase copper containing 3	0.7589	48

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	45.9 nTPM
Breast	63.0 nTPM
Adipose tissue	28.8 nTPM

Alveolar cells type 1	16.0 nTPM
Lung	16.0 nTPM

Alveolar cells type 2	38.3 nTPM
Lung	38.3 nTPM

Astrocytes	43.4 nTPM
Brain	43.4 nTPM

B-cells	2.5 nTPM
Kidney	9.5 nTPM
Breast	8.1 nTPM
Liver	4.1 nTPM
PBMC	1.4 nTPM
Adipose tissue	1.0 nTPM
Stomach	0.5 nTPM
Lymph node	0.2 nTPM
Colon	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM

Dendritic cells	3.5 nTPM
PBMC	8.0 nTPM
Breast	2.2 nTPM
Adipose tissue	0.5 nTPM

Endothelial cells	56.9 nTPM
Prostate	134.3 nTPM
Skin	127.7 nTPM
Breast	83.0 nTPM
Ovary	76.9 nTPM
Eye	76.1 nTPM
Esophagus	65.8 nTPM
Pancreas	46.4 nTPM
Placenta	36.9 nTPM
Heart muscle	32.3 nTPM
Testis	32.3 nTPM
Lung	31.2 nTPM
Skeletal muscle	27.1 nTPM
Endometrium	17.0 nTPM
Liver	9.2 nTPM

Fibroblasts	40.1 nTPM
Ovary	126.8 nTPM
Prostate	68.6 nTPM
Lung	47.0 nTPM
Stomach	43.0 nTPM
Heart muscle	35.2 nTPM
Esophagus	34.4 nTPM
Skin	26.8 nTPM
Adipose tissue	26.7 nTPM
Placenta	15.8 nTPM
Breast	9.0 nTPM
Skeletal muscle	7.6 nTPM

Intestinal goblet cells	3.9 nTPM
Rectum	6.6 nTPM
Colon	2.9 nTPM
Small intestine	2.1 nTPM

Macrophages	20.8 nTPM
PBMC	245.5 nTPM
Breast	10.6 nTPM
Testis	9.2 nTPM
Lung	8.4 nTPM
Prostate	8.1 nTPM
Esophagus	7.9 nTPM
Endometrium	6.8 nTPM
Skeletal muscle	6.7 nTPM
Adipose tissue	4.4 nTPM
Kidney	3.8 nTPM
Ovary	0.8 nTPM
Pancreas	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM
Stomach	0.0 nTPM

Paneth cells	18.7 nTPM
Colon	29.6 nTPM
Rectum	26.4 nTPM
Small intestine	0.0 nTPM

Plasma cells	3.3 nTPM
Esophagus	12.3 nTPM
Stomach	4.4 nTPM
Adipose tissue	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM

Respiratory ciliated cells	17.6 nTPM
Bronchus	29.3 nTPM
Lung	5.8 nTPM

Smooth muscle cells	448.7 nTPM
Prostate	995.9 nTPM
Heart muscle	913.4 nTPM
Skin	906.2 nTPM
Esophagus	558.2 nTPM
Endometrium	472.2 nTPM
Breast	298.5 nTPM
Adipose tissue	292.7 nTPM
Skeletal muscle	282.3 nTPM
Pancreas	93.3 nTPM
Ovary	88.9 nTPM
Bronchus	34.0 nTPM

T-cells	3.2 nTPM
Skin	19.8 nTPM
Endometrium	7.5 nTPM
Lung	6.1 nTPM
Breast	4.6 nTPM
Liver	3.8 nTPM
Skeletal muscle	3.5 nTPM
Kidney	3.4 nTPM
Ovary	2.2 nTPM
PBMC	1.4 nTPM
Colon	0.5 nTPM
Lymph node	0.5 nTPM
Adipose tissue	0.4 nTPM
Stomach	0.2 nTPM
Bone marrow	0.1 nTPM
Prostate	0.0 nTPM
Esophagus	0.0 nTPM
Spleen	0.0 nTPM

Basal keratinocytes	20.5 nTPM
Skin	20.5 nTPM

Basal prostatic cells	27.2 nTPM
Prostate	27.2 nTPM

Basal respiratory cells	24.8 nTPM
Bronchus	24.8 nTPM

Basal squamous epithelial cells	1.2 nTPM
Esophagus	1.2 nTPM

Undifferentiated cells	4.2 nTPM
Rectum	7.7 nTPM
Colon	3.3 nTPM
Small intestine	1.4 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC