Single cell type - PDE4A - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	PDE4A
Gene descriptionⁱ Full gene name according to HGNC.	Phosphodiesterase 4A
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes FDA approved drug targets Metabolic proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	6

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Spermatids - Spermatogenesis (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Late spermatids, Langerhans cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (HMC-1, NB-4)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Late spermatids, Langerhans cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

PDE4A is part of cluster 25 Spermatids - Spermatogenesis with confidenceⁱ 1
458 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
CHRNE	Cholinergic receptor nicotinic epsilon subunit	0.6568	25
ATP1B3	ATPase Na+/K+ transporting subunit beta 3	0.5915	41
PDZD8	PDZ domain containing 8	0.5668	25
MLLT11	MLLT11 transcription factor 7 cofactor	0.5620	25
CXCL16	C-X-C motif chemokine ligand 16	0.5525	25
FAM102B	Family with sequence similarity 102 member B	0.5497	41
CSGALNACT2	Chondroitin sulfate N-acetylgalactosaminyltransferase 2	0.5286	41
TTLL9	Tubulin tyrosine ligase like 9	0.5214	25
HABP4	Hyaluronan binding protein 4	0.5152	25
SYAP1	Synapse associated protein 1	0.5110	24
TSC1	TSC complex subunit 1	0.5107	25
DHX57	DExH-box helicase 57	0.5071	25
PHF20	PHD finger protein 20	0.5054	24
CSNK1G2	Casein kinase 1 gamma 2	0.5043	25
SRGN	Serglycin	0.5015	41

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	9.4 nTPM
Breast	10.1 nTPM
Adipose tissue	8.6 nTPM

Alveolar cells type 1	3.2 nTPM
Lung	3.2 nTPM

Alveolar cells type 2	11.6 nTPM
Lung	11.6 nTPM

Astrocytes	6.2 nTPM
Brain	6.2 nTPM

B-cells	10.8 nTPM
Adipose tissue	39.6 nTPM
Colon	36.7 nTPM
Breast	13.4 nTPM
Stomach	10.0 nTPM
Liver	2.8 nTPM
Lymph node	2.5 nTPM
Spleen	1.7 nTPM
PBMC	1.4 nTPM
Kidney	0.0 nTPM
Bone marrow	0.0 nTPM

Dendritic cells	58.9 nTPM
Adipose tissue	132.8 nTPM
Breast	43.8 nTPM
PBMC	0.0 nTPM

Endothelial cells	8.6 nTPM
Skin	16.9 nTPM
Liver	16.5 nTPM
Pancreas	15.5 nTPM
Eye	13.7 nTPM
Testis	11.9 nTPM
Heart muscle	10.7 nTPM
Skeletal muscle	9.1 nTPM
Breast	8.8 nTPM
Esophagus	5.8 nTPM
Endometrium	4.9 nTPM
Prostate	3.1 nTPM
Ovary	2.7 nTPM
Placenta	0.6 nTPM
Lung	0.0 nTPM

Fibroblasts	12.1 nTPM
Adipose tissue	24.2 nTPM
Skin	23.1 nTPM
Skeletal muscle	22.9 nTPM
Esophagus	16.2 nTPM
Placenta	14.0 nTPM
Breast	11.8 nTPM
Stomach	7.4 nTPM
Prostate	7.2 nTPM
Ovary	2.4 nTPM
Lung	2.2 nTPM
Heart muscle	2.0 nTPM

Intestinal goblet cells	5.1 nTPM
Rectum	11.3 nTPM
Colon	3.6 nTPM
Small intestine	0.4 nTPM

Macrophages	53.9 nTPM
Adipose tissue	216.1 nTPM
Stomach	99.6 nTPM
Skeletal muscle	78.9 nTPM
Prostate	69.9 nTPM
Esophagus	55.1 nTPM
Endometrium	51.5 nTPM
Spleen	46.7 nTPM
Testis	41.2 nTPM
Pancreas	38.3 nTPM
Lung	30.0 nTPM
Breast	28.7 nTPM
Ovary	22.7 nTPM
Bone marrow	20.0 nTPM
Kidney	9.9 nTPM
PBMC	0.0 nTPM

Paneth cells	3.8 nTPM
Rectum	11.3 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM

Plasma cells	27.0 nTPM
Adipose tissue	103.2 nTPM
Stomach	15.2 nTPM
Bone marrow	6.6 nTPM
Esophagus	6.1 nTPM
Spleen	4.0 nTPM

Respiratory ciliated cells	6.9 nTPM
Lung	11.7 nTPM
Bronchus	2.1 nTPM

Smooth muscle cells	10.2 nTPM
Skin	22.3 nTPM
Adipose tissue	17.6 nTPM
Skeletal muscle	17.1 nTPM
Endometrium	13.6 nTPM
Breast	11.7 nTPM
Prostate	9.1 nTPM
Pancreas	8.5 nTPM
Esophagus	7.8 nTPM
Ovary	3.8 nTPM
Heart muscle	1.1 nTPM
Bronchus	0.0 nTPM

T-cells	37.0 nTPM
Adipose tissue	112.0 nTPM
Colon	96.9 nTPM
Skin	87.5 nTPM
Endometrium	80.9 nTPM
Skeletal muscle	43.8 nTPM
Prostate	42.6 nTPM
Breast	31.6 nTPM
Stomach	28.0 nTPM
Ovary	22.3 nTPM
Liver	21.7 nTPM
Spleen	14.0 nTPM
Esophagus	13.1 nTPM
Lung	10.6 nTPM
Bone marrow	9.8 nTPM
Kidney	5.5 nTPM
Lymph node	5.1 nTPM
PBMC	3.3 nTPM

Basal prostatic cells	2.6 nTPM
Prostate	2.6 nTPM

Basal squamous epithelial cells	1.8 nTPM
Esophagus	1.8 nTPM

Collecting duct cells	9.2 nTPM
Kidney	9.2 nTPM

Cytotrophoblasts	0.5 nTPM
Placenta	0.5 nTPM

Undifferentiated cells	6.8 nTPM
Rectum	12.2 nTPM
Colon	7.4 nTPM
Small intestine	0.7 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC