Single cell type - ACSL4 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ACSL4
Gene descriptionⁱ Full gene name according to HGNC.	Acyl-CoA synthetase long chain family member 4
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Enzymes Human disease related genes Metabolic proteins Plasma proteins Potential drug targets
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	11

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Granulocytes - Receptor signaling (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (granulocytes, Glandular and luminal cells, Alveolar cells type 2)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (HMC-1)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (granulocytes, Glandular and luminal cells, Alveolar cells type 2)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

ACSL4 is part of cluster 60 Granulocytes - Receptor signaling with confidenceⁱ 1
79 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
SLC18A2	Solute carrier family 18 member A2	0.8057	60
AL136295.1	Novel protein	0.7050	60
AP000781.2	Novel protein	0.6917	60
AC244197.3	Novel protein	0.6904	60
RAB44	RAB44, member RAS oncogene family	0.6862	60
AC018630.2	PRH1-PRR4 readthrough	0.6858	60
OR1D2	Olfactory receptor family 1 subfamily D member 2	0.6850	60
OR14J1	Olfactory receptor family 14 subfamily J member 1	0.6770	60
HTD2	Hydroxyacyl-thioester dehydratase type 2	0.6704	60
AC011462.1	Novel protein	0.6688	60
HDC	Histidine decarboxylase	0.6688	60
AC093155.3	Novel protein	0.6680	60
CPA3	Carboxypeptidase A3	0.6638	60
TPSD1	Tryptase delta 1	0.6631	60
MS4A2	Membrane spanning 4-domains A2	0.6619	60

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	27.9 nTPM
Adipose tissue	33.9 nTPM
Breast	21.9 nTPM

Alveolar cells type 1	76.9 nTPM
Lung	76.9 nTPM

Astrocytes	29.0 nTPM
Brain	29.0 nTPM

B-cells	19.5 nTPM
Spleen	26.3 nTPM
Liver	25.1 nTPM
Adipose tissue	23.6 nTPM
Lymph node	22.1 nTPM
Breast	21.3 nTPM
Stomach	20.9 nTPM
Bone marrow	16.4 nTPM
Kidney	14.3 nTPM
PBMC	14.3 nTPM
Colon	11.2 nTPM

Basal keratinocytes	22.3 nTPM
Skin	22.3 nTPM

Dendritic cells	18.3 nTPM
Adipose tissue	23.3 nTPM
PBMC	20.0 nTPM
Breast	11.7 nTPM

Endothelial cells	29.8 nTPM
Lung	62.4 nTPM
Endometrium	59.8 nTPM
Prostate	41.2 nTPM
Pancreas	34.7 nTPM
Heart muscle	34.4 nTPM
Eye	30.7 nTPM
Esophagus	29.5 nTPM
Ovary	24.2 nTPM
Liver	20.2 nTPM
Skeletal muscle	20.2 nTPM
Testis	19.3 nTPM
Breast	18.7 nTPM
Skin	15.4 nTPM
Placenta	6.2 nTPM

Fibroblasts	46.0 nTPM
Lung	114.0 nTPM
Prostate	96.2 nTPM
Stomach	87.6 nTPM
Skeletal muscle	44.9 nTPM
Adipose tissue	39.7 nTPM
Breast	26.2 nTPM
Heart muscle	25.7 nTPM
Esophagus	23.5 nTPM
Ovary	19.5 nTPM
Skin	17.1 nTPM
Placenta	12.1 nTPM

Intestinal goblet cells	5.7 nTPM
Small intestine	9.2 nTPM
Rectum	4.3 nTPM
Colon	3.6 nTPM

Macrophages	67.8 nTPM
Lung	141.8 nTPM
Adipose tissue	141.8 nTPM
Stomach	120.1 nTPM
Spleen	117.1 nTPM
Bone marrow	71.2 nTPM
Skeletal muscle	64.1 nTPM
Esophagus	61.4 nTPM
Kidney	49.9 nTPM
Prostate	49.2 nTPM
Endometrium	48.5 nTPM
Pancreas	46.0 nTPM
Ovary	40.2 nTPM
Testis	38.3 nTPM
Breast	27.1 nTPM
PBMC	0.0 nTPM

Paneth cells	3.0 nTPM
Small intestine	7.5 nTPM
Rectum	1.5 nTPM
Colon	0.0 nTPM

Plasma cells	36.1 nTPM
Esophagus	43.0 nTPM
Stomach	42.7 nTPM
Bone marrow	39.5 nTPM
Spleen	29.0 nTPM
Adipose tissue	26.4 nTPM

Respiratory ciliated cells	12.2 nTPM
Lung	19.0 nTPM
Bronchus	5.4 nTPM

Smooth muscle cells	27.9 nTPM
Ovary	80.7 nTPM
Pancreas	40.3 nTPM
Prostate	26.3 nTPM
Heart muscle	24.5 nTPM
Endometrium	24.2 nTPM
Bronchus	23.6 nTPM
Adipose tissue	23.6 nTPM
Skeletal muscle	20.3 nTPM
Esophagus	18.6 nTPM
Breast	14.4 nTPM
Skin	10.5 nTPM

T-cells	25.2 nTPM
Lung	83.6 nTPM
Esophagus	39.3 nTPM
Prostate	37.9 nTPM
Endometrium	31.1 nTPM
Stomach	27.9 nTPM
Adipose tissue	27.5 nTPM
Skeletal muscle	25.4 nTPM
Spleen	23.7 nTPM
Bone marrow	22.7 nTPM
Ovary	22.3 nTPM
Lymph node	17.8 nTPM
Breast	17.2 nTPM
Liver	11.3 nTPM
PBMC	11.2 nTPM
Skin	11.1 nTPM
Colon	10.5 nTPM
Kidney	8.9 nTPM

Cholangiocytes	15.7 nTPM
Liver	15.7 nTPM

Collecting duct cells	73.5 nTPM
Kidney	73.5 nTPM

Cone photoreceptor cells	2.1 nTPM
Eye	2.1 nTPM

Cytotrophoblasts	7.3 nTPM
Placenta	7.3 nTPM

Undifferentiated cells	2.7 nTPM
Colon	3.6 nTPM
Small intestine	3.4 nTPM
Rectum	1.2 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC