Single cell type - P4HA2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	P4HA2
Gene descriptionⁱ Full gene name according to HGNC.	Prolyl 4-hydroxylase subunit alpha 2
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Enzymes Human disease related genes Metabolic proteins Potential drug targets
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	15

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Respiratory epithelial cells - Mucosal defense (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Basal respiratory cells, Skeletal myocytes, Endometrial stromal cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in single
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (HHSteC, U-87 MG)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Basal respiratory cells, Skeletal myocytes, Endometrial stromal cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

P4HA2 is part of cluster 26 Respiratory epithelial cells - Mucosal defense with confidenceⁱ 1
293 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
STRA6	Signaling receptor and transporter of retinol STRA6	0.6698	33
SGCB	Sarcoglycan beta	0.6445	50
KCTD11	Potassium channel tetramerization domain containing 11	0.6204	26
FAP	Fibroblast activation protein alpha	0.6080	50
P4HA1	Prolyl 4-hydroxylase subunit alpha 1	0.6029	26
FAM114A1	Family with sequence similarity 114 member A1	0.6010	33
TNC	Tenascin C	0.5844	26
KRTAP19-3	Keratin associated protein 19-3	0.5841	26
AMTN	Amelotin	0.5761	26
ANGPTL4	Angiopoietin like 4	0.5737	26
PLOD2	Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2	0.5734	33
COL7A1	Collagen type VII alpha 1 chain	0.5717	26
CYP27C1	Cytochrome P450 family 27 subfamily C member 1	0.5694	36
SFRP4	Secreted frizzled related protein 4	0.5689	50
MMP14	Matrix metallopeptidase 14	0.5662	38

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	13.0 nTPM
Breast	18.5 nTPM
Adipose tissue	7.6 nTPM

Alveolar cells type 1	35.3 nTPM
Lung	35.3 nTPM

Alveolar cells type 2	10.3 nTPM
Lung	10.3 nTPM

Astrocytes	7.5 nTPM
Brain	7.5 nTPM

B-cells	1.1 nTPM
Breast	5.3 nTPM
Liver	3.7 nTPM
Spleen	1.0 nTPM
Bone marrow	0.8 nTPM
Colon	0.0 nTPM
Kidney	0.0 nTPM
PBMC	0.0 nTPM
Adipose tissue	0.0 nTPM
Lymph node	0.0 nTPM
Stomach	0.0 nTPM

Dendritic cells	0.3 nTPM
Adipose tissue	0.5 nTPM
Breast	0.4 nTPM
PBMC	0.0 nTPM

Endothelial cells	12.2 nTPM
Placenta	27.3 nTPM
Pancreas	23.1 nTPM
Skeletal muscle	17.6 nTPM
Endometrium	16.5 nTPM
Prostate	12.4 nTPM
Eye	11.3 nTPM
Ovary	10.9 nTPM
Heart muscle	10.2 nTPM
Lung	9.3 nTPM
Skin	9.1 nTPM
Breast	9.0 nTPM
Esophagus	7.8 nTPM
Liver	3.7 nTPM
Testis	2.4 nTPM

Fibroblasts	61.6 nTPM
Stomach	112.9 nTPM
Placenta	111.6 nTPM
Skeletal muscle	91.6 nTPM
Esophagus	83.5 nTPM
Adipose tissue	71.6 nTPM
Prostate	42.2 nTPM
Skin	42.1 nTPM
Breast	40.0 nTPM
Lung	34.7 nTPM
Heart muscle	33.4 nTPM
Ovary	13.9 nTPM

Intestinal goblet cells	18.6 nTPM
Small intestine	24.7 nTPM
Colon	15.7 nTPM
Rectum	15.4 nTPM

Macrophages	4.4 nTPM
Pancreas	20.4 nTPM
Lung	11.9 nTPM
Skeletal muscle	8.5 nTPM
Endometrium	6.2 nTPM
Prostate	4.6 nTPM
Testis	3.5 nTPM
Esophagus	3.1 nTPM
Kidney	2.3 nTPM
Breast	1.6 nTPM
Ovary	1.3 nTPM
Bone marrow	1.2 nTPM
Adipose tissue	0.7 nTPM
PBMC	0.0 nTPM
Spleen	0.0 nTPM
Stomach	0.0 nTPM

Paneth cells	41.0 nTPM
Small intestine	56.3 nTPM
Colon	39.6 nTPM
Rectum	27.1 nTPM

Plasma cells	3.0 nTPM
Esophagus	6.1 nTPM
Stomach	4.5 nTPM
Adipose tissue	1.9 nTPM
Bone marrow	1.7 nTPM
Spleen	0.7 nTPM

Respiratory ciliated cells	71.9 nTPM
Bronchus	78.1 nTPM
Lung	65.6 nTPM

Smooth muscle cells	47.6 nTPM
Pancreas	324.3 nTPM
Bronchus	39.4 nTPM
Prostate	33.4 nTPM
Endometrium	29.9 nTPM
Skin	17.4 nTPM
Adipose tissue	16.4 nTPM
Breast	15.4 nTPM
Skeletal muscle	14.4 nTPM
Ovary	13.8 nTPM
Heart muscle	10.3 nTPM
Esophagus	9.3 nTPM

T-cells	4.8 nTPM
Prostate	23.6 nTPM
Skin	23.5 nTPM
Endometrium	9.5 nTPM
Colon	6.2 nTPM
Skeletal muscle	4.9 nTPM
Lung	4.5 nTPM
Kidney	2.7 nTPM
Ovary	2.2 nTPM
Breast	1.4 nTPM
Liver	1.1 nTPM
Stomach	0.6 nTPM
Adipose tissue	0.3 nTPM
Spleen	0.2 nTPM
PBMC	0.1 nTPM
Bone marrow	0.1 nTPM
Esophagus	0.0 nTPM
Lymph node	0.0 nTPM

Basal keratinocytes	16.8 nTPM
Skin	16.8 nTPM

Basal prostatic cells	43.6 nTPM
Prostate	43.6 nTPM

Cholangiocytes	8.6 nTPM
Liver	8.6 nTPM

Collecting duct cells	11.0 nTPM
Kidney	11.0 nTPM

Undifferentiated cells	13.8 nTPM
Colon	17.0 nTPM
Rectum	13.2 nTPM
Small intestine	11.1 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC