Single cell type - NSF - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	NSF
Gene descriptionⁱ Full gene name according to HGNC.	N-ethylmaleimide sensitive factor, vesicle fusing ATPase
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes Transporters
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	6

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Neurons & Oligodendrocytes - Synaptic function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Inhibitory neurons, Excitatory neurons)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Inhibitory neurons, Excitatory neurons)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

NSF is part of cluster 8 Neurons & Oligodendrocytes - Synaptic function with confidenceⁱ 1
719 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
HCN1	Hyperpolarization activated cyclic nucleotide gated potassium channel 1	0.7176	8
KLHL29	Kelch like family member 29	0.7030	8
ZMAT4	Zinc finger matrin-type 4	0.7019	8
DLGAP1	DLG associated protein 1	0.6930	8
SETBP1	SET binding protein 1	0.6917	8
BEND6	BEN domain containing 6	0.6850	8
SAMD5	Sterile alpha motif domain containing 5	0.6823	8
FBXL17	F-box and leucine rich repeat protein 17	0.6819	8
TRPC4	Transient receptor potential cation channel subfamily C member 4	0.6801	8
OVCH1	Ovochymase 1	0.6782	8
ELMOD1	ELMO domain containing 1	0.6781	8
SLC4A10	Solute carrier family 4 member 10	0.6704	21
CACNG2	Calcium voltage-gated channel auxiliary subunit gamma 2	0.6701	8
KSR2	Kinase suppressor of ras 2	0.6672	21
FMN1	Formin 1	0.6562	8

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	16.3 nTPM
Breast	16.5 nTPM
Adipose tissue	16.1 nTPM

Alveolar cells type 1	9.6 nTPM
Lung	9.6 nTPM

Alveolar cells type 2	11.3 nTPM
Lung	11.3 nTPM

Astrocytes	54.9 nTPM
Brain	54.9 nTPM

B-cells	28.2 nTPM
Adipose tissue	49.1 nTPM
Stomach	42.2 nTPM
Breast	38.8 nTPM
Spleen	33.2 nTPM
Lymph node	29.0 nTPM
Bone marrow	28.6 nTPM
Liver	19.3 nTPM
Kidney	14.3 nTPM
PBMC	14.3 nTPM
Colon	12.8 nTPM

Dendritic cells	18.8 nTPM
Adipose tissue	22.5 nTPM
Breast	17.8 nTPM
PBMC	16.0 nTPM

Endothelial cells	11.5 nTPM
Heart muscle	22.1 nTPM
Eye	19.4 nTPM
Endometrium	15.7 nTPM
Ovary	12.6 nTPM
Esophagus	12.4 nTPM
Testis	11.6 nTPM
Pancreas	11.6 nTPM
Liver	10.1 nTPM
Skeletal muscle	10.1 nTPM
Breast	9.7 nTPM
Skin	8.1 nTPM
Placenta	6.8 nTPM
Lung	6.2 nTPM
Prostate	5.1 nTPM

Fibroblasts	10.9 nTPM
Adipose tissue	15.8 nTPM
Ovary	15.2 nTPM
Placenta	13.0 nTPM
Skeletal muscle	13.0 nTPM
Breast	12.8 nTPM
Heart muscle	11.6 nTPM
Esophagus	9.2 nTPM
Lung	7.8 nTPM
Skin	7.4 nTPM
Stomach	7.4 nTPM
Prostate	6.2 nTPM

Intestinal goblet cells	26.3 nTPM
Rectum	36.1 nTPM
Small intestine	27.3 nTPM
Colon	15.7 nTPM

Macrophages	20.5 nTPM
Stomach	29.4 nTPM
Bone marrow	27.5 nTPM
Kidney	27.2 nTPM
Ovary	26.9 nTPM
Spleen	24.9 nTPM
Skeletal muscle	23.8 nTPM
Endometrium	21.2 nTPM
Adipose tissue	20.7 nTPM
Breast	20.4 nTPM
Testis	19.2 nTPM
Lung	17.3 nTPM
Esophagus	17.3 nTPM
Prostate	16.0 nTPM
Pancreas	15.3 nTPM
PBMC	0.0 nTPM

Paneth cells	17.4 nTPM
Rectum	24.5 nTPM
Colon	16.5 nTPM
Small intestine	11.3 nTPM

Plasma cells	21.7 nTPM
Bone marrow	29.7 nTPM
Adipose tissue	24.5 nTPM
Stomach	24.5 nTPM
Spleen	23.4 nTPM
Esophagus	6.1 nTPM

Respiratory ciliated cells	8.7 nTPM
Bronchus	13.1 nTPM
Lung	4.4 nTPM

Smooth muscle cells	12.9 nTPM
Heart muscle	23.1 nTPM
Pancreas	17.8 nTPM
Endometrium	13.9 nTPM
Adipose tissue	13.9 nTPM
Prostate	13.1 nTPM
Bronchus	13.0 nTPM
Esophagus	10.9 nTPM
Skeletal muscle	10.3 nTPM
Breast	9.6 nTPM
Ovary	8.6 nTPM
Skin	7.3 nTPM

T-cells	14.8 nTPM
Adipose tissue	24.8 nTPM
Breast	21.7 nTPM
Bone marrow	21.2 nTPM
Esophagus	19.7 nTPM
Stomach	19.3 nTPM
Skeletal muscle	18.2 nTPM
Spleen	17.0 nTPM
Lymph node	15.2 nTPM
Prostate	14.2 nTPM
Endometrium	14.0 nTPM
Liver	13.3 nTPM
Kidney	11.6 nTPM
PBMC	11.3 nTPM
Lung	10.6 nTPM
Skin	8.8 nTPM
Colon	8.5 nTPM
Ovary	2.2 nTPM

Basal keratinocytes	4.0 nTPM
Skin	4.0 nTPM

Basal prostatic cells	3.6 nTPM
Prostate	3.6 nTPM

Basal respiratory cells	6.7 nTPM
Bronchus	6.7 nTPM

Cholangiocytes	7.2 nTPM
Liver	7.2 nTPM

Undifferentiated cells	20.6 nTPM
Rectum	24.8 nTPM
Small intestine	18.6 nTPM
Colon	18.5 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC