Single cell type - DGKD - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	DGKD
Gene descriptionⁱ Full gene name according to HGNC.	Diacylglycerol kinase delta
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes Metabolic proteins Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	5

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Neurons & Oligodendrocytes - Synaptic function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Gastric mucus-secreting cells, Inhibitory neurons)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (HDLM-2, HeLa, U-698)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Gastric mucus-secreting cells, Inhibitory neurons)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

DGKD is part of cluster 8 Neurons & Oligodendrocytes - Synaptic function with confidenceⁱ 0.99
719 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
EYA4	EYA transcriptional coactivator and phosphatase 4	0.6938	8
PRELID2	PRELI domain containing 2	0.6806	8
TRPC3	Transient receptor potential cation channel subfamily C member 3	0.6773	8
FGF13	Fibroblast growth factor 13	0.6599	8
TACR1	Tachykinin receptor 1	0.6309	8
PTCHD4	Patched domain containing 4	0.6263	8
NXPH2	Neurexophilin 2	0.6206	8
KIAA1109	KIAA1109	0.6204	8
GRIN3A	Glutamate ionotropic receptor NMDA type subunit 3A	0.6092	8
MYO16	Myosin XVI	0.6083	8
GAD2	Glutamate decarboxylase 2	0.6075	8
SPHKAP	SPHK1 interactor, AKAP domain containing	0.6030	8
MAGEB17	MAGE family member B17	0.5965	8
SLC6A1	Solute carrier family 6 member 1	0.5829	8
SGCZ	Sarcoglycan zeta	0.5827	8

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	18.9 nTPM
Breast	21.3 nTPM
Adipose tissue	16.4 nTPM

Alveolar cells type 1	32.1 nTPM
Lung	32.1 nTPM

Alveolar cells type 2	46.2 nTPM
Lung	46.2 nTPM

Astrocytes	27.1 nTPM
Brain	27.1 nTPM

B-cells	61.1 nTPM
Adipose tissue	96.8 nTPM
Lymph node	88.4 nTPM
PBMC	74.3 nTPM
Spleen	66.1 nTPM
Breast	62.8 nTPM
Bone marrow	60.5 nTPM
Stomach	58.2 nTPM
Colon	47.9 nTPM
Kidney	38.1 nTPM
Liver	18.4 nTPM

Dendritic cells	62.0 nTPM
Adipose tissue	87.2 nTPM
Breast	74.7 nTPM
PBMC	23.9 nTPM

Endothelial cells	19.1 nTPM
Ovary	40.5 nTPM
Prostate	37.0 nTPM
Heart muscle	31.5 nTPM
Skeletal muscle	27.4 nTPM
Lung	24.9 nTPM
Pancreas	23.1 nTPM
Skin	21.7 nTPM
Endometrium	18.1 nTPM
Breast	12.9 nTPM
Eye	11.3 nTPM
Esophagus	8.3 nTPM
Placenta	5.7 nTPM
Testis	3.8 nTPM
Liver	0.9 nTPM

Fibroblasts	9.7 nTPM
Stomach	17.8 nTPM
Heart muscle	15.7 nTPM
Lung	15.7 nTPM
Ovary	11.4 nTPM
Prostate	9.3 nTPM
Skeletal muscle	9.0 nTPM
Esophagus	6.5 nTPM
Breast	5.9 nTPM
Adipose tissue	5.6 nTPM
Placenta	5.5 nTPM
Skin	4.3 nTPM

Intestinal goblet cells	32.8 nTPM
Colon	47.2 nTPM
Rectum	29.8 nTPM
Small intestine	21.4 nTPM

Macrophages	28.7 nTPM
PBMC	122.7 nTPM
Adipose tissue	61.5 nTPM
Skeletal muscle	52.3 nTPM
Stomach	43.9 nTPM
Esophagus	23.6 nTPM
Spleen	19.7 nTPM
Lung	19.3 nTPM
Breast	14.9 nTPM
Testis	14.0 nTPM
Endometrium	14.0 nTPM
Bone marrow	13.8 nTPM
Ovary	12.9 nTPM
Kidney	9.1 nTPM
Prostate	9.1 nTPM
Pancreas	0.0 nTPM

Paneth cells	13.5 nTPM
Colon	23.1 nTPM
Rectum	13.7 nTPM
Small intestine	3.7 nTPM

Plasma cells	34.7 nTPM
Adipose tissue	85.2 nTPM
Esophagus	24.6 nTPM
Stomach	23.6 nTPM
Spleen	23.4 nTPM
Bone marrow	16.5 nTPM

Respiratory ciliated cells	4.8 nTPM
Lung	7.3 nTPM
Bronchus	2.3 nTPM

Smooth muscle cells	21.2 nTPM
Heart muscle	43.2 nTPM
Skeletal muscle	27.9 nTPM
Esophagus	27.9 nTPM
Endometrium	25.2 nTPM
Ovary	24.0 nTPM
Breast	21.6 nTPM
Skin	20.2 nTPM
Prostate	18.7 nTPM
Adipose tissue	15.6 nTPM
Bronchus	4.8 nTPM
Pancreas	4.7 nTPM

T-cells	32.5 nTPM
Skeletal muscle	55.8 nTPM
Stomach	42.4 nTPM
Adipose tissue	41.8 nTPM
Endometrium	40.7 nTPM
Spleen	38.5 nTPM
Lymph node	36.2 nTPM
Lung	34.9 nTPM
PBMC	34.8 nTPM
Esophagus	32.8 nTPM
Bone marrow	32.2 nTPM
Ovary	31.1 nTPM
Breast	30.5 nTPM
Skin	27.6 nTPM
Liver	27.4 nTPM
Colon	18.1 nTPM
Prostate	14.2 nTPM
Kidney	13.1 nTPM

Basal keratinocytes	8.9 nTPM
Skin	8.9 nTPM

Basal prostatic cells	6.4 nTPM
Prostate	6.4 nTPM

Basal squamous epithelial cells	4.6 nTPM
Esophagus	4.6 nTPM

Cone photoreceptor cells	62.6 nTPM
Eye	62.6 nTPM

Undifferentiated cells	20.1 nTPM
Small intestine	21.7 nTPM
Colon	19.7 nTPM
Rectum	18.9 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC