Single cell type - TOP2B - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	TOP2B
Gene descriptionⁱ Full gene name according to HGNC.	DNA topoisomerase II beta
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Enzymes FDA approved drug targets Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	3

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Spermatocytes & Spermatogonia - Spermatogenesis (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low cell type specificity
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (REH)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Low cell type specificity

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

TOP2B is part of cluster 13 Spermatocytes & Spermatogonia - Spermatogenesis with confidenceⁱ 1
231 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
RNF34	Ring finger protein 34	0.5840	13
DIS3L	DIS3 like exosome 3'-5' exoribonuclease	0.5551	52
NIPBL	NIPBL cohesin loading factor	0.5551	10
ZFR	Zinc finger RNA binding protein	0.5510	10
TCF24	Transcription factor 24	0.5358	13
ARIH2	Ariadne RBR E3 ubiquitin protein ligase 2	0.5328	13
TPR	Translocated promoter region, nuclear basket protein	0.5303	13
SUZ12	SUZ12 polycomb repressive complex 2 subunit	0.5175	10
ZBTB12	Zinc finger and BTB domain containing 12	0.4856	10
ANKRD31	Ankyrin repeat domain 31	0.4831	13
KIAA2026	KIAA2026	0.4761	52
UBE3A	Ubiquitin protein ligase E3A	0.4741	10
GML	Glycosylphosphatidylinositol anchored molecule like	0.4740	13
CCP110	Centriolar coiled-coil protein 110	0.4735	49
YLPM1	YLP motif containing 1	0.4734	52

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	36.4 nTPM
Breast	36.7 nTPM
Adipose tissue	36.2 nTPM

Alveolar cells type 1	64.1 nTPM
Lung	64.1 nTPM

Alveolar cells type 2	62.7 nTPM
Lung	62.7 nTPM

Astrocytes	65.3 nTPM
Brain	65.3 nTPM

B-cells	47.3 nTPM
Lymph node	62.9 nTPM
Bone marrow	62.1 nTPM
Spleen	57.8 nTPM
Adipose tissue	56.1 nTPM
PBMC	55.7 nTPM
Colon	44.8 nTPM
Stomach	43.6 nTPM
Breast	34.7 nTPM
Liver	31.7 nTPM
Kidney	23.8 nTPM

Dendritic cells	65.8 nTPM
Adipose tissue	91.3 nTPM
Breast	58.3 nTPM
PBMC	47.8 nTPM

Endothelial cells	36.6 nTPM
Endometrium	58.9 nTPM
Ovary	50.0 nTPM
Heart muscle	49.6 nTPM
Prostate	41.7 nTPM
Skeletal muscle	40.1 nTPM
Placenta	36.4 nTPM
Pancreas	34.7 nTPM
Breast	34.5 nTPM
Lung	31.2 nTPM
Testis	29.6 nTPM
Eye	29.1 nTPM
Skin	28.8 nTPM
Esophagus	26.8 nTPM
Liver	21.1 nTPM

Fibroblasts	40.2 nTPM
Ovary	81.8 nTPM
Lung	50.3 nTPM
Heart muscle	43.5 nTPM
Skeletal muscle	41.2 nTPM
Skin	40.0 nTPM
Prostate	39.0 nTPM
Breast	37.7 nTPM
Adipose tissue	35.0 nTPM
Placenta	34.9 nTPM
Esophagus	29.5 nTPM
Stomach	8.9 nTPM

Intestinal goblet cells	32.3 nTPM
Rectum	41.8 nTPM
Small intestine	33.7 nTPM
Colon	21.5 nTPM

Macrophages	45.3 nTPM
Spleen	83.9 nTPM
Bone marrow	81.2 nTPM
Adipose tissue	62.0 nTPM
Stomach	58.6 nTPM
Skeletal muscle	58.1 nTPM
Ovary	49.9 nTPM
Endometrium	46.1 nTPM
Lung	43.5 nTPM
Kidney	42.4 nTPM
Testis	37.2 nTPM
Prostate	33.2 nTPM
Breast	33.2 nTPM
Esophagus	31.5 nTPM
Pancreas	17.8 nTPM
PBMC	0.0 nTPM

Paneth cells	22.2 nTPM
Rectum	37.9 nTPM
Small intestine	18.8 nTPM
Colon	9.9 nTPM

Plasma cells	46.0 nTPM
Adipose tissue	75.5 nTPM
Esophagus	55.5 nTPM
Stomach	36.1 nTPM
Spleen	34.7 nTPM
Bone marrow	28.0 nTPM

Respiratory ciliated cells	39.2 nTPM
Bronchus	40.5 nTPM
Lung	37.9 nTPM

Smooth muscle cells	51.0 nTPM
Heart muscle	101.7 nTPM
Ovary	75.1 nTPM
Bronchus	61.8 nTPM
Endometrium	60.8 nTPM
Adipose tissue	46.4 nTPM
Skeletal muscle	41.5 nTPM
Prostate	41.3 nTPM
Esophagus	38.8 nTPM
Skin	38.7 nTPM
Breast	36.7 nTPM
Pancreas	17.7 nTPM

T-cells	53.7 nTPM
Adipose tissue	73.1 nTPM
Skeletal muscle	69.9 nTPM
Endometrium	68.1 nTPM
PBMC	67.8 nTPM
Lung	62.3 nTPM
Lymph node	60.2 nTPM
Esophagus	58.9 nTPM
Spleen	58.4 nTPM
Liver	52.6 nTPM
Breast	51.7 nTPM
Colon	49.0 nTPM
Bone marrow	48.4 nTPM
Prostate	47.3 nTPM
Stomach	40.8 nTPM
Kidney	39.7 nTPM
Skin	38.7 nTPM
Ovary	26.7 nTPM

Basal keratinocytes	39.1 nTPM
Skin	39.1 nTPM

Basal prostatic cells	47.9 nTPM
Prostate	47.9 nTPM

Basal respiratory cells	30.9 nTPM
Bronchus	30.9 nTPM

Basal squamous epithelial cells	31.3 nTPM
Esophagus	31.3 nTPM

Undifferentiated cells	39.2 nTPM
Rectum	48.2 nTPM
Small intestine	39.5 nTPM
Colon	29.8 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC