Single cell type - EDN1 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	EDN1
Gene descriptionⁱ Full gene name according to HGNC.	Endothelin 1
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Cancer-related genes Candidate cardiovascular disease genes Disease related genes Human disease related genes Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Secreted
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	1

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Epithelial cell types - Mixed function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Basal prostatic cells, Ductal cells, Glandular and luminal cells, Pancreatic endocrine cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enriched (non-classical monocyte)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in single
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (EFO-21, HUVEC TERT2, T-47d, TIME)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level
Extracellular locationⁱ A possible secretion of the protein was predicted based on N-terminal signal sequence (signal peptide) predictions and transmembrane region predictions, and subsequently annotated for its local or systemic secretion based on literature, function, and protein and RNA expression data. Read more	Protein predicted to be secreted

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Basal prostatic cells, Ductal cells, Glandular and luminal cells, Pancreatic endocrine cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

EDN1 is part of cluster 22 Epithelial cell types - Mixed function with confidenceⁱ 1
298 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
KRTAP1-1	Keratin associated protein 1-1	0.7744	22
GADD45A	Growth arrest and DNA damage inducible alpha	0.7569	22
TM4SF1	Transmembrane 4 L six family member 1	0.7230	22
SHB	SH2 domain containing adaptor protein B	0.7072	22
TNFRSF12A	TNF receptor superfamily member 12A	0.7006	22
HSPB1	Heat shock protein family B (small) member 1	0.6415	22
SH2D3A	SH2 domain containing 3A	0.6397	22
FGF19	Fibroblast growth factor 19	0.6326	1
KRTAP4-12	Keratin associated protein 4-12	0.6261	22
MMP7	Matrix metallopeptidase 7	0.6226	1
TMEM51	Transmembrane protein 51	0.6205	1
FAM110C	Family with sequence similarity 110 member C	0.6204	22
ERRFI1	ERBB receptor feedback inhibitor 1	0.6187	22
NEURL3	Neuralized E3 ubiquitin protein ligase 3	0.6163	1
KRTAP3-1	Keratin associated protein 3-1	0.6157	22

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	190.7 nTPM
Adipose tissue	341.4 nTPM
Breast	40.0 nTPM

Alveolar cells type 1	173.1 nTPM
Lung	173.1 nTPM

Alveolar cells type 2	23.8 nTPM
Lung	23.8 nTPM

Astrocytes	2.9 nTPM
Brain	2.9 nTPM

B-cells	0.8 nTPM
Adipose tissue	2.0 nTPM
Colon	1.6 nTPM
Liver	1.3 nTPM
Breast	1.3 nTPM
Stomach	0.9 nTPM
Lymph node	0.5 nTPM
Spleen	0.3 nTPM
Kidney	0.0 nTPM
PBMC	0.0 nTPM
Bone marrow	0.0 nTPM

Basal squamous epithelial cells	151.4 nTPM
Esophagus	151.4 nTPM

Dendritic cells	0.8 nTPM
Breast	2.2 nTPM
Adipose tissue	0.4 nTPM
PBMC	0.0 nTPM

Endothelial cells	236.8 nTPM
Pancreas	1081.0 nTPM
Liver	375.1 nTPM
Testis	349.9 nTPM
Endometrium	256.9 nTPM
Eye	202.3 nTPM
Skeletal muscle	201.8 nTPM
Placenta	193.2 nTPM
Skin	166.9 nTPM
Lung	140.4 nTPM
Esophagus	95.1 nTPM
Breast	89.4 nTPM
Prostate	76.5 nTPM
Heart muscle	44.0 nTPM
Ovary	42.2 nTPM

Fibroblasts	9.0 nTPM
Prostate	29.2 nTPM
Lung	21.2 nTPM
Stomach	20.8 nTPM
Skin	9.3 nTPM
Heart muscle	5.5 nTPM
Placenta	3.1 nTPM
Breast	3.1 nTPM
Skeletal muscle	2.7 nTPM
Adipose tissue	2.5 nTPM
Esophagus	1.3 nTPM
Ovary	0.6 nTPM

Intestinal goblet cells	43.8 nTPM
Colon	100.8 nTPM
Small intestine	19.5 nTPM
Rectum	11.3 nTPM

Macrophages	5.3 nTPM
Endometrium	35.2 nTPM
Prostate	20.7 nTPM
Stomach	5.9 nTPM
Lung	3.5 nTPM
Testis	2.9 nTPM
Breast	2.9 nTPM
Pancreas	2.5 nTPM
Skeletal muscle	2.4 nTPM
Kidney	2.3 nTPM
Adipose tissue	1.4 nTPM
Ovary	0.5 nTPM
PBMC	0.0 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM

Paneth cells	145.7 nTPM
Colon	177.9 nTPM
Small intestine	157.7 nTPM
Rectum	101.7 nTPM

Plasma cells	0.4 nTPM
Adipose tissue	1.3 nTPM
Stomach	0.4 nTPM
Spleen	0.3 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM

Respiratory ciliated cells	27.4 nTPM
Bronchus	54.8 nTPM
Lung	0.0 nTPM

Smooth muscle cells	21.8 nTPM
Ovary	66.0 nTPM
Bronchus	54.4 nTPM
Prostate	49.4 nTPM
Pancreas	29.1 nTPM
Endometrium	14.8 nTPM
Breast	6.8 nTPM
Skin	6.7 nTPM
Heart muscle	6.5 nTPM
Skeletal muscle	3.8 nTPM
Esophagus	1.5 nTPM
Adipose tissue	0.7 nTPM

Cholangiocytes	8.6 nTPM
Liver	8.6 nTPM

Collecting duct cells	38.6 nTPM
Kidney	38.6 nTPM

Cytotrophoblasts	1.8 nTPM
Placenta	1.8 nTPM

Distal enterocytes	189.7 nTPM
Colon	270.8 nTPM
Rectum	108.6 nTPM

T-cells	9.7 nTPM
Prostate	80.4 nTPM
Endometrium	43.9 nTPM
Colon	9.0 nTPM
Skin	7.8 nTPM
Kidney	6.2 nTPM
Lung	6.1 nTPM
Ovary	4.5 nTPM
Breast	2.9 nTPM
Liver	1.4 nTPM
Skeletal muscle	1.0 nTPM
Spleen	0.5 nTPM
Adipose tissue	0.3 nTPM
PBMC	0.2 nTPM
Lymph node	0.2 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM
Stomach	0.0 nTPM

Undifferentiated cells	196.6 nTPM
Colon	451.1 nTPM
Small intestine	107.1 nTPM
Rectum	31.7 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC