Single cell type - PPIE - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	PPIE
Gene descriptionⁱ Full gene name according to HGNC.	Peptidylprolyl isomerase E
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes Metabolic proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	7

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Spermatids - Spermatogenesis (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low cell type specificity
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Low cell type specificity

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

PPIE is part of cluster 31 Spermatids - Spermatogenesis with confidenceⁱ 1
413 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
NSMCE1	NSE1 homolog, SMC5-SMC6 complex component	0.5993	31
TMEM186	Transmembrane protein 186	0.5690	31
PIN1	Peptidylprolyl cis/trans isomerase, NIMA-interacting 1	0.5457	31
UBE2J2	Ubiquitin conjugating enzyme E2 J2	0.5295	66
TAF9	TATA-box binding protein associated factor 9	0.5218	31
ELAC1	ElaC ribonuclease Z 1	0.5191	39
R3HCC1	R3H domain and coiled-coil containing 1	0.5028	46
FAM104B	Family with sequence similarity 104 member B	0.5018	49
ELP5	Elongator acetyltransferase complex subunit 5	0.5005	31
MRPL28	Mitochondrial ribosomal protein L28	0.4881	31
PRPSAP2	Phosphoribosyl pyrophosphate synthetase associated protein 2	0.4880	66
MED4	Mediator complex subunit 4	0.4867	39
C1orf50	Chromosome 1 open reading frame 50	0.4675	31
SNAPIN	SNAP associated protein	0.4623	26
PHPT1	Phosphohistidine phosphatase 1	0.4590	39

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	28.2 nTPM
Breast	37.2 nTPM
Adipose tissue	19.3 nTPM

Alveolar cells type 1	32.1 nTPM
Lung	32.1 nTPM

Alveolar cells type 2	36.7 nTPM
Lung	36.7 nTPM

Astrocytes	10.7 nTPM
Brain	10.7 nTPM

B-cells	37.0 nTPM
Liver	57.9 nTPM
Kidney	47.6 nTPM
PBMC	45.7 nTPM
Breast	40.2 nTPM
Colon	38.3 nTPM
Spleen	32.5 nTPM
Bone marrow	28.6 nTPM
Adipose tissue	27.0 nTPM
Stomach	26.3 nTPM
Lymph node	25.7 nTPM

Dendritic cells	39.3 nTPM
Breast	52.5 nTPM
Adipose tissue	33.6 nTPM
PBMC	31.8 nTPM

Endothelial cells	28.7 nTPM
Liver	44.9 nTPM
Testis	44.2 nTPM
Placenta	42.6 nTPM
Eye	34.8 nTPM
Breast	29.4 nTPM
Esophagus	29.3 nTPM
Ovary	26.8 nTPM
Skin	26.3 nTPM
Heart muscle	24.8 nTPM
Prostate	23.4 nTPM
Endometrium	21.2 nTPM
Pancreas	19.3 nTPM
Skeletal muscle	19.0 nTPM
Lung	15.6 nTPM

Fibroblasts	34.0 nTPM
Placenta	48.8 nTPM
Adipose tissue	38.6 nTPM
Ovary	37.4 nTPM
Heart muscle	36.8 nTPM
Breast	35.9 nTPM
Esophagus	33.3 nTPM
Skin	32.8 nTPM
Skeletal muscle	29.2 nTPM
Lung	27.9 nTPM
Stomach	26.7 nTPM
Prostate	26.6 nTPM

Intestinal goblet cells	32.9 nTPM
Rectum	37.6 nTPM
Colon	31.5 nTPM
Small intestine	29.7 nTPM

Macrophages	30.0 nTPM
Lung	48.5 nTPM
Ovary	41.7 nTPM
Testis	41.2 nTPM
Breast	37.2 nTPM
Kidney	36.3 nTPM
Pancreas	33.2 nTPM
Bone marrow	31.2 nTPM
Endometrium	29.4 nTPM
PBMC	27.3 nTPM
Prostate	26.3 nTPM
Adipose tissue	25.0 nTPM
Esophagus	23.6 nTPM
Spleen	20.7 nTPM
Skeletal muscle	16.3 nTPM
Stomach	11.7 nTPM

Paneth cells	24.1 nTPM
Rectum	32.3 nTPM
Small intestine	30.0 nTPM
Colon	9.9 nTPM

Plasma cells	31.7 nTPM
Spleen	49.1 nTPM
Bone marrow	41.2 nTPM
Stomach	33.2 nTPM
Esophagus	18.4 nTPM
Adipose tissue	16.7 nTPM

Respiratory ciliated cells	63.4 nTPM
Lung	69.9 nTPM
Bronchus	57.0 nTPM

Smooth muscle cells	40.1 nTPM
Pancreas	65.0 nTPM
Breast	51.9 nTPM
Heart muscle	48.5 nTPM
Esophagus	46.5 nTPM
Skin	43.0 nTPM
Ovary	39.4 nTPM
Prostate	35.7 nTPM
Skeletal muscle	32.0 nTPM
Bronchus	29.3 nTPM
Adipose tissue	28.2 nTPM
Endometrium	22.1 nTPM

T-cells	36.6 nTPM
Liver	57.6 nTPM
PBMC	56.4 nTPM
Lung	45.6 nTPM
Bone marrow	44.4 nTPM
Ovary	42.2 nTPM
Kidney	41.1 nTPM
Colon	40.4 nTPM
Breast	37.0 nTPM
Stomach	34.7 nTPM
Skin	34.5 nTPM
Spleen	34.1 nTPM
Adipose tissue	30.0 nTPM
Lymph node	29.9 nTPM
Endometrium	27.5 nTPM
Prostate	23.6 nTPM
Skeletal muscle	23.6 nTPM
Esophagus	19.7 nTPM

Basal keratinocytes	24.9 nTPM
Skin	24.9 nTPM

Basal prostatic cells	43.1 nTPM
Prostate	43.1 nTPM

Basal respiratory cells	69.8 nTPM
Bronchus	69.8 nTPM

Basal squamous epithelial cells	23.9 nTPM
Esophagus	23.9 nTPM

Undifferentiated cells	35.9 nTPM
Small intestine	37.8 nTPM
Colon	36.2 nTPM
Rectum	33.5 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC