Single cell type - WFDC2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	WFDC2
Gene descriptionⁱ Full gene name according to HGNC.	WAP four-disulfide core domain 2
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Cancer-related genes
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Secreted
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	4

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Respiratory epithelial cells - Mucosal defense (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Club cells, Glandular and luminal cells, Basal respiratory cells, Urothelial cells, Endometrial ciliated cells, Respiratory ciliated cells, Collecting duct cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Not detected in immune cells
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Not detected
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Group enriched (A-431, CAPAN-2, EFO-21, HaCaT, RPTEC TERT1)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in some
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level
Extracellular locationⁱ A possible secretion of the protein was predicted based on N-terminal signal sequence (signal peptide) predictions and transmembrane region predictions, and subsequently annotated for its local or systemic secretion based on literature, function, and protein and RNA expression data. Read more	Protein predicted to be secreted

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Club cells, Glandular and luminal cells, Basal respiratory cells, Urothelial cells, Endometrial ciliated cells, Respiratory ciliated cells, Collecting duct cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

WFDC2 is part of cluster 26 Respiratory epithelial cells - Mucosal defense with confidenceⁱ 1
293 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
SLPI	Secretory leukocyte peptidase inhibitor	0.9061	26
UPK1B	Uroplakin 1B	0.8932	26
IL36A	Interleukin 36 alpha	0.8903	26
CXCL17	C-X-C motif chemokine ligand 17	0.8876	26
SRD5A3	Steroid 5 alpha-reductase 3	0.8863	26
BIK	BCL2 interacting killer	0.8605	26
SCGB3A1	Secretoglobin family 3A member 1	0.8513	26
BPIFA1	BPI fold containing family A member 1	0.8428	26
SLC6A14	Solute carrier family 6 member 14	0.8421	26
LYPD2	LY6/PLAUR domain containing 2	0.8415	26
BPIFB1	BPI fold containing family B member 1	0.8407	26
ATP12A	ATPase H+/K+ transporting non-gastric alpha2 subunit	0.8389	26
PI3	Peptidase inhibitor 3	0.8389	26
MSLN	Mesothelin	0.8382	26
CXCL6	C-X-C motif chemokine ligand 6	0.8367	26

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	2.8 nTPM
Breast	5.6 nTPM
Adipose tissue	0.0 nTPM

Alveolar cells type 1	169.9 nTPM
Lung	169.9 nTPM

Alveolar cells type 2	273.8 nTPM
Lung	273.8 nTPM

Astrocytes	2.6 nTPM
Brain	2.6 nTPM

B-cells	4.2 nTPM
Colon	19.2 nTPM
Breast	16.0 nTPM
Kidney	4.8 nTPM
Liver	0.9 nTPM
Stomach	0.9 nTPM
PBMC	0.0 nTPM
Adipose tissue	0.0 nTPM
Lymph node	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM

Dendritic cells	4.3 nTPM
Breast	12.8 nTPM
PBMC	0.0 nTPM
Adipose tissue	0.0 nTPM

Endothelial cells	21.7 nTPM
Endometrium	115.4 nTPM
Prostate	99.7 nTPM
Breast	57.4 nTPM
Lung	21.8 nTPM
Skeletal muscle	2.5 nTPM
Eye	2.4 nTPM
Skin	1.8 nTPM
Esophagus	1.0 nTPM
Heart muscle	0.9 nTPM
Placenta	0.6 nTPM
Testis	0.6 nTPM
Liver	0.0 nTPM
Pancreas	0.0 nTPM
Ovary	0.0 nTPM

Enteroendocrine cells	422.7 nTPM
Rectum	536.3 nTPM
Colon	309.1 nTPM

Fibroblasts	23.4 nTPM
Placenta	81.9 nTPM
Prostate	68.6 nTPM
Lung	64.8 nTPM
Breast	16.5 nTPM
Esophagus	9.7 nTPM
Stomach	6.0 nTPM
Heart muscle	5.4 nTPM
Skin	2.3 nTPM
Adipose tissue	1.1 nTPM
Skeletal muscle	0.6 nTPM
Ovary	0.4 nTPM

Glandular and luminal cells	4817.5 nTPM
Endometrium	4817.5 nTPM

Intestinal goblet cells	1764.7 nTPM
Rectum	3303.8 nTPM
Colon	1963.3 nTPM
Small intestine	26.9 nTPM

Macrophages	26.8 nTPM
Endometrium	271.0 nTPM
Prostate	79.2 nTPM
Lung	24.5 nTPM
Kidney	11.4 nTPM
Breast	10.4 nTPM
Esophagus	4.8 nTPM
Ovary	0.2 nTPM
PBMC	0.0 nTPM
Testis	0.0 nTPM
Pancreas	0.0 nTPM
Skeletal muscle	0.0 nTPM
Adipose tissue	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM
Stomach	0.0 nTPM

Paneth cells	424.6 nTPM
Rectum	644.6 nTPM
Colon	520.4 nTPM
Small intestine	108.8 nTPM

Plasma cells	0.8 nTPM
Stomach	2.0 nTPM
Adipose tissue	1.9 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM

Respiratory ciliated cells	2935.3 nTPM
Bronchus	4694.3 nTPM
Lung	1176.2 nTPM

Cholangiocytes	94.4 nTPM
Liver	94.4 nTPM

Cone photoreceptor cells	22.9 nTPM
Eye	22.9 nTPM

Distal enterocytes	239.8 nTPM
Colon	243.0 nTPM
Rectum	236.7 nTPM

Distal tubular cells	221.1 nTPM
Kidney	221.1 nTPM

Smooth muscle cells	319.1 nTPM
Bronchus	3115.9 nTPM
Prostate	157.6 nTPM
Endometrium	112.1 nTPM
Esophagus	55.8 nTPM
Breast	22.3 nTPM
Skin	19.1 nTPM
Pancreas	13.7 nTPM
Skeletal muscle	5.0 nTPM
Adipose tissue	4.3 nTPM
Ovary	3.9 nTPM
Heart muscle	0.8 nTPM

T-cells	36.8 nTPM
Endometrium	473.5 nTPM
Prostate	56.8 nTPM
Kidney	43.1 nTPM
Lung	18.2 nTPM
Breast	16.8 nTPM
Colon	14.7 nTPM
Skin	1.4 nTPM
Stomach	0.4 nTPM
Liver	0.0 nTPM
PBMC	0.0 nTPM
Skeletal muscle	0.0 nTPM
Ovary	0.0 nTPM
Adipose tissue	0.0 nTPM
Esophagus	0.0 nTPM
Lymph node	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM

Undifferentiated cells	318.2 nTPM
Rectum	584.4 nTPM
Colon	355.7 nTPM
Small intestine	14.3 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC