Single cell type - MID1 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	MID1
Gene descriptionⁱ Full gene name according to HGNC.	Midline 1
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Enzymes Human disease related genes Metabolic proteins Potential drug targets
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	12

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Astrocytes - Nervous system maintenance (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Astrocytes, Basal squamous epithelial cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Not detected in immune cells
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Not detected
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (A549, AF22)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Astrocytes, Basal squamous epithelial cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

MID1 is part of cluster 64 Astrocytes - Nervous system maintenance with confidenceⁱ 1
169 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
SMAD9	SMAD family member 9	0.6032	44
RALGAPA2	Ral GTPase activating protein catalytic subunit alpha 2	0.5714	44
UNC5B	Unc-5 netrin receptor B	0.5651	62
GLIS3	GLIS family zinc finger 3	0.5403	64
BCL9	BCL9 transcription coactivator	0.5267	44
CABLES1	Cdk5 and Abl enzyme substrate 1	0.5170	64
FAM189A2	Family with sequence similarity 189 member A2	0.5154	64
DPY19L3	Dpy-19 like C-mannosyltransferase 3	0.5138	68
STON2	Stonin 2	0.5066	64
EYA2	EYA transcriptional coactivator and phosphatase 2	0.5063	64
FZD6	Frizzled class receptor 6	0.5057	44
GTF2IRD1	GTF2I repeat domain containing 1	0.4870	9
PLEKHA7	Pleckstrin homology domain containing A7	0.4833	64
PHKA1	Phosphorylase kinase regulatory subunit alpha 1	0.4825	64
WWTR1	WW domain containing transcription regulator 1	0.4786	14

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	16.5 nTPM
Adipose tissue	23.4 nTPM
Breast	9.6 nTPM

Alveolar cells type 1	35.3 nTPM
Lung	35.3 nTPM

Alveolar cells type 2	20.7 nTPM
Lung	20.7 nTPM

B-cells	0.7 nTPM
Stomach	3.7 nTPM
Spleen	2.1 nTPM
Adipose tissue	0.5 nTPM
Lymph node	0.4 nTPM
Colon	0.0 nTPM
Kidney	0.0 nTPM
Liver	0.0 nTPM
PBMC	0.0 nTPM
Bone marrow	0.0 nTPM
Breast	0.0 nTPM

Basal keratinocytes	21.4 nTPM
Skin	21.4 nTPM

Dendritic cells	1.3 nTPM
PBMC	4.0 nTPM
Adipose tissue	0.0 nTPM
Breast	0.0 nTPM

Endothelial cells	25.4 nTPM
Skin	48.4 nTPM
Liver	44.9 nTPM
Prostate	37.5 nTPM
Endometrium	32.0 nTPM
Heart muscle	28.3 nTPM
Esophagus	24.3 nTPM
Placenta	23.9 nTPM
Ovary	23.9 nTPM
Pancreas	23.1 nTPM
Breast	17.7 nTPM
Lung	15.6 nTPM
Skeletal muscle	15.1 nTPM
Testis	13.6 nTPM
Eye	7.3 nTPM

Fibroblasts	16.2 nTPM
Esophagus	31.6 nTPM
Stomach	26.7 nTPM
Skin	22.8 nTPM
Prostate	20.5 nTPM
Adipose tissue	17.2 nTPM
Skeletal muscle	12.9 nTPM
Placenta	12.3 nTPM
Lung	11.1 nTPM
Ovary	10.1 nTPM
Heart muscle	7.7 nTPM
Breast	5.0 nTPM

Intestinal goblet cells	9.0 nTPM
Rectum	13.9 nTPM
Small intestine	9.6 nTPM
Colon	3.6 nTPM

Macrophages	1.2 nTPM
Prostate	5.7 nTPM
Endometrium	3.8 nTPM
Stomach	2.9 nTPM
Skeletal muscle	1.8 nTPM
Lung	0.9 nTPM
Kidney	0.8 nTPM
Adipose tissue	0.6 nTPM
Ovary	0.5 nTPM
Breast	0.5 nTPM
PBMC	0.0 nTPM
Testis	0.0 nTPM
Pancreas	0.0 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM

Paneth cells	17.2 nTPM
Rectum	27.7 nTPM
Colon	16.5 nTPM
Small intestine	7.5 nTPM

Plasma cells	0.2 nTPM
Spleen	0.6 nTPM
Stomach	0.5 nTPM
Adipose tissue	0.0 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM

Respiratory ciliated cells	19.3 nTPM
Bronchus	22.5 nTPM
Lung	16.0 nTPM

Smooth muscle cells	3.3 nTPM
Prostate	9.4 nTPM
Endometrium	6.6 nTPM
Ovary	3.9 nTPM
Heart muscle	2.9 nTPM
Adipose tissue	2.7 nTPM
Skin	2.5 nTPM
Pancreas	2.0 nTPM
Bronchus	1.9 nTPM
Skeletal muscle	1.6 nTPM
Esophagus	1.5 nTPM
Breast	0.8 nTPM

T-cells	0.8 nTPM
Prostate	4.7 nTPM
Endometrium	4.7 nTPM
Skin	1.4 nTPM
Colon	1.0 nTPM
Skeletal muscle	0.5 nTPM
Breast	0.5 nTPM
Liver	0.2 nTPM
Lymph node	0.2 nTPM
Stomach	0.1 nTPM
Kidney	0.0 nTPM
Lung	0.0 nTPM
PBMC	0.0 nTPM
Ovary	0.0 nTPM
Adipose tissue	0.0 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM

Cholangiocytes	8.6 nTPM
Liver	8.6 nTPM

Cone photoreceptor cells	18.8 nTPM
Eye	18.8 nTPM

Cytotrophoblasts	9.1 nTPM
Placenta	9.1 nTPM

Distal enterocytes	22.6 nTPM
Rectum	27.3 nTPM
Colon	17.8 nTPM

Undifferentiated cells	20.0 nTPM
Rectum	26.3 nTPM
Small intestine	18.9 nTPM
Colon	14.9 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC