Single cell type - TRIM35 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	TRIM35
Gene descriptionⁱ Full gene name according to HGNC.	Tripartite motif containing 35
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	3

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Myeloid cells - Hemostasis (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Basal squamous epithelial cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (ASC diff)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Basal squamous epithelial cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

TRIM35 is part of cluster 51 Myeloid cells - Hemostasis with confidenceⁱ 1
155 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
MAP3K7CL	MAP3K7 C-terminal like	0.6274	51
GRAP2	GRB2 related adaptor protein 2	0.5974	51
CMTM5	CKLF like MARVEL transmembrane domain containing 5	0.5817	51
AQP10	Aquaporin 10	0.5742	51
ALOX12	Arachidonate 12-lipoxygenase, 12S type	0.5619	51
SCGB1C1	Secretoglobin family 1C member 1	0.5606	51
PF4	Platelet factor 4	0.5548	51
LY6G6F	Lymphocyte antigen 6 family member G6F	0.5544	51
SPIN2B	Spindlin family member 2B	0.5543	51
HBG1	Hemoglobin subunit gamma 1	0.5541	51
GP9	Glycoprotein IX platelet	0.5527	51
PPBP	Pro-platelet basic protein	0.5504	51
TUBB1	Tubulin beta 1 class VI	0.5490	51
CLEC1B	C-type lectin domain family 1 member B	0.5441	51
ITGB3	Integrin subunit beta 3	0.5441	51

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	9.4 nTPM
Adipose tissue	10.3 nTPM
Breast	8.5 nTPM

Alveolar cells type 1	3.2 nTPM
Lung	3.2 nTPM

Alveolar cells type 2	4.5 nTPM
Lung	4.5 nTPM

Astrocytes	5.2 nTPM
Brain	5.2 nTPM

B-cells	4.4 nTPM
Kidney	14.3 nTPM
PBMC	5.7 nTPM
Stomach	4.5 nTPM
Lymph node	3.8 nTPM
Bone marrow	3.2 nTPM
Adipose tissue	3.0 nTPM
Liver	2.8 nTPM
Spleen	2.8 nTPM
Breast	2.6 nTPM
Colon	1.6 nTPM

Dendritic cells	3.2 nTPM
Breast	5.8 nTPM
Adipose tissue	3.9 nTPM
PBMC	0.0 nTPM

Endothelial cells	6.7 nTPM
Liver	24.7 nTPM
Skin	7.4 nTPM
Skeletal muscle	7.4 nTPM
Esophagus	7.3 nTPM
Ovary	7.0 nTPM
Heart muscle	6.3 nTPM
Lung	6.2 nTPM
Breast	5.9 nTPM
Placenta	5.1 nTPM
Endometrium	4.6 nTPM
Testis	4.1 nTPM
Pancreas	3.9 nTPM
Prostate	2.2 nTPM
Eye	1.6 nTPM

Fibroblasts	6.6 nTPM
Heart muscle	17.5 nTPM
Adipose tissue	14.8 nTPM
Skin	11.3 nTPM
Breast	7.5 nTPM
Esophagus	4.8 nTPM
Skeletal muscle	4.7 nTPM
Stomach	4.4 nTPM
Placenta	2.7 nTPM
Ovary	2.6 nTPM
Lung	1.2 nTPM
Prostate	1.2 nTPM

Intestinal goblet cells	2.7 nTPM
Rectum	4.3 nTPM
Colon	2.9 nTPM
Small intestine	1.1 nTPM

Macrophages	6.9 nTPM
PBMC	54.6 nTPM
Esophagus	9.4 nTPM
Spleen	6.2 nTPM
Pancreas	5.1 nTPM
Testis	4.0 nTPM
Bone marrow	3.7 nTPM
Lung	3.5 nTPM
Endometrium	3.4 nTPM
Ovary	3.3 nTPM
Breast	2.9 nTPM
Kidney	2.3 nTPM
Adipose tissue	2.2 nTPM
Skeletal muscle	1.6 nTPM
Prostate	1.2 nTPM
Stomach	0.0 nTPM

Paneth cells	2.0 nTPM
Rectum	6.1 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM

Plasma cells	4.8 nTPM
Stomach	7.9 nTPM
Bone marrow	6.6 nTPM
Adipose tissue	5.8 nTPM
Spleen	4.0 nTPM
Esophagus	0.0 nTPM

Respiratory ciliated cells	0.9 nTPM
Bronchus	1.7 nTPM
Lung	0.0 nTPM

Smooth muscle cells	5.5 nTPM
Heart muscle	17.3 nTPM
Skin	7.0 nTPM
Adipose tissue	6.8 nTPM
Breast	5.4 nTPM
Skeletal muscle	4.4 nTPM
Ovary	4.3 nTPM
Endometrium	4.0 nTPM
Pancreas	3.6 nTPM
Prostate	3.2 nTPM
Esophagus	3.1 nTPM
Bronchus	1.9 nTPM

T-cells	6.9 nTPM
Skin	14.7 nTPM
Ovary	13.4 nTPM
Bone marrow	10.9 nTPM
Lymph node	9.3 nTPM
Adipose tissue	8.7 nTPM
Lung	7.6 nTPM
Breast	7.6 nTPM
Liver	7.3 nTPM
Spleen	7.0 nTPM
PBMC	6.8 nTPM
Kidney	6.2 nTPM
Colon	5.2 nTPM
Skeletal muscle	4.7 nTPM
Stomach	4.6 nTPM
Endometrium	3.4 nTPM
Prostate	0.0 nTPM
Esophagus	0.0 nTPM

Basal keratinocytes	20.9 nTPM
Skin	20.9 nTPM

Basal prostatic cells	6.0 nTPM
Prostate	6.0 nTPM

Cholangiocytes	8.6 nTPM
Liver	8.6 nTPM

Collecting duct cells	12.9 nTPM
Kidney	12.9 nTPM

Undifferentiated cells	3.5 nTPM
Small intestine	3.7 nTPM
Colon	3.5 nTPM
Rectum	3.2 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC