Single cell type - SPAG1 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	SPAG1
Gene descriptionⁱ Full gene name according to HGNC.	Sperm associated antigen 1
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Human disease related genes
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	4

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Ciliated cells - Cilium organization (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Respiratory ciliated cells, Endometrial ciliated cells, Early spermatids, Spermatocytes)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in single
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (A-431)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Respiratory ciliated cells, Endometrial ciliated cells, Early spermatids, Spermatocytes)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

SPAG1 is part of cluster 49 Ciliated cells - Cilium organization with confidenceⁱ 1
457 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
SPAG8	Sperm associated antigen 8	0.7650	49
DNAI1	Dynein axonemal intermediate chain 1	0.7617	49
DNAH9	Dynein axonemal heavy chain 9	0.7599	49
SPEF1	Sperm flagellar 1	0.7581	49
C20orf85	Chromosome 20 open reading frame 85	0.7197	49
MAPK15	Mitogen-activated protein kinase 15	0.7193	49
PIFO	Primary cilia formation	0.7146	49
RSPH1	Radial spoke head component 1	0.7134	49
ANKUB1	Ankyrin repeat and ubiquitin domain containing 1	0.7113	49
LRRC46	Leucine rich repeat containing 46	0.7099	49
CAPSL	Calcyphosine like	0.7070	49
STMND1	Stathmin domain containing 1	0.7024	49
RSPH9	Radial spoke head component 9	0.6950	49
FAM183A	Family with sequence similarity 183 member A	0.6946	49
KCNRG	Potassium channel regulator	0.6860	49

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	7.7 nTPM
Adipose tissue	8.0 nTPM
Breast	7.4 nTPM

Alveolar cells type 1	48.1 nTPM
Lung	48.1 nTPM

Alveolar cells type 2	22.8 nTPM
Lung	22.8 nTPM

Astrocytes	18.9 nTPM
Brain	18.9 nTPM

B-cells	7.7 nTPM
Adipose tissue	17.6 nTPM
Stomach	16.8 nTPM
Breast	9.4 nTPM
Lymph node	8.5 nTPM
Liver	7.2 nTPM
PBMC	5.7 nTPM
Spleen	5.2 nTPM
Bone marrow	4.9 nTPM
Colon	1.6 nTPM
Kidney	0.0 nTPM

Dendritic cells	19.0 nTPM
Adipose tissue	38.8 nTPM
Breast	18.2 nTPM
PBMC	0.0 nTPM

Endothelial cells	6.9 nTPM
Endometrium	10.3 nTPM
Heart muscle	9.4 nTPM
Lung	9.3 nTPM
Prostate	8.9 nTPM
Skin	8.8 nTPM
Esophagus	8.8 nTPM
Pancreas	7.7 nTPM
Eye	7.3 nTPM
Breast	6.9 nTPM
Skeletal muscle	5.3 nTPM
Testis	4.4 nTPM
Ovary	4.3 nTPM
Placenta	2.9 nTPM
Liver	1.8 nTPM

Fibroblasts	5.0 nTPM
Heart muscle	10.5 nTPM
Lung	8.9 nTPM
Skeletal muscle	6.0 nTPM
Skin	5.1 nTPM
Breast	4.7 nTPM
Prostate	4.6 nTPM
Adipose tissue	4.6 nTPM
Esophagus	4.3 nTPM
Placenta	2.7 nTPM
Ovary	2.0 nTPM
Stomach	1.5 nTPM

Intestinal goblet cells	14.9 nTPM
Rectum	23.6 nTPM
Colon	18.6 nTPM
Small intestine	2.4 nTPM

Macrophages	3.9 nTPM
PBMC	13.6 nTPM
Lung	5.7 nTPM
Adipose tissue	5.7 nTPM
Esophagus	4.8 nTPM
Prostate	4.6 nTPM
Endometrium	4.1 nTPM
Skeletal muscle	4.1 nTPM
Spleen	3.1 nTPM
Breast	3.1 nTPM
Testis	2.9 nTPM
Bone marrow	2.5 nTPM
Kidney	2.3 nTPM
Ovary	1.3 nTPM
Pancreas	0.0 nTPM
Stomach	0.0 nTPM

Paneth cells	17.7 nTPM
Colon	29.6 nTPM
Rectum	19.8 nTPM
Small intestine	3.7 nTPM

Plasma cells	15.9 nTPM
Adipose tissue	29.1 nTPM
Stomach	23.1 nTPM
Esophagus	12.3 nTPM
Spleen	8.2 nTPM
Bone marrow	6.6 nTPM

Respiratory ciliated cells	198.6 nTPM
Lung	212.8 nTPM
Bronchus	184.2 nTPM

Smooth muscle cells	6.4 nTPM
Bronchus	29.3 nTPM
Heart muscle	14.6 nTPM
Prostate	5.0 nTPM
Endometrium	4.4 nTPM
Breast	4.2 nTPM
Ovary	3.4 nTPM
Skin	2.8 nTPM
Pancreas	2.5 nTPM
Adipose tissue	2.5 nTPM
Skeletal muscle	2.1 nTPM
Esophagus	0.0 nTPM

T-cells	12.0 nTPM
Adipose tissue	35.2 nTPM
Endometrium	23.0 nTPM
Prostate	19.0 nTPM
Skin	17.5 nTPM
Lung	13.6 nTPM
Esophagus	13.1 nTPM
Skeletal muscle	12.7 nTPM
Stomach	12.7 nTPM
Colon	11.9 nTPM
Breast	11.9 nTPM
Liver	10.1 nTPM
Spleen	6.1 nTPM
Ovary	4.5 nTPM
Bone marrow	4.0 nTPM
Kidney	3.4 nTPM
Lymph node	3.4 nTPM
PBMC	1.5 nTPM

Basal keratinocytes	5.6 nTPM
Skin	5.6 nTPM

Basal prostatic cells	9.6 nTPM
Prostate	9.6 nTPM

Basal respiratory cells	18.1 nTPM
Bronchus	18.1 nTPM

Basal squamous epithelial cells	2.2 nTPM
Esophagus	2.2 nTPM

Undifferentiated cells	20.1 nTPM
Rectum	31.3 nTPM
Colon	28.2 nTPM
Small intestine	0.8 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC