Single cell type - DOT1L - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	DOT1L
Gene descriptionⁱ Full gene name according to HGNC.	DOT1 like histone lysine methyltransferase
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes Metabolic proteins Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	5

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Endometrium - Transcription (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Cone photoreceptor cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Not detected in immune cells
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Not detected
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Cone photoreceptor cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

DOT1L is part of cluster 44 Endometrium - Transcription with confidenceⁱ 1
491 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
HGS	Hepatocyte growth factor-regulated tyrosine kinase substrate	0.6083	44
ARID3B	AT-rich interaction domain 3B	0.6056	44
OPA3	Outer mitochondrial membrane lipid metabolism regulator OPA3	0.5708	52
MAFG	MAF bZIP transcription factor G	0.5223	22
LIPG	Lipase G, endothelial type	0.5160	44
IFNK	Interferon kappa	0.5071	44
BX276092.9	Chromosome X open reading frame 49-like	0.5004	44
SPAG9	Sperm associated antigen 9	0.4887	44
CLEC20A	C-type lectin domain containing 20A	0.4847	44
MGRN1	Mahogunin ring finger 1	0.4787	52
ABI2	Abl interactor 2	0.4785	44
ATP13A3	ATPase 13A3	0.4739	41
TRIM25	Tripartite motif containing 25	0.4735	44
ANKLE2	Ankyrin repeat and LEM domain containing 2	0.4717	44
AGO3	Argonaute RISC catalytic component 3	0.4708	52

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	9.3 nTPM
Adipose tissue	11.5 nTPM
Breast	7.2 nTPM

Alveolar cells type 1	32.1 nTPM
Lung	32.1 nTPM

Alveolar cells type 2	16.3 nTPM
Lung	16.3 nTPM

Astrocytes	12.4 nTPM
Brain	12.4 nTPM

B-cells	9.5 nTPM
Colon	19.2 nTPM
Breast	13.4 nTPM
Liver	13.0 nTPM
Lymph node	11.2 nTPM
Adipose tissue	9.6 nTPM
Stomach	8.6 nTPM
Spleen	6.9 nTPM
Kidney	4.8 nTPM
PBMC	4.3 nTPM
Bone marrow	4.1 nTPM

Dendritic cells	15.9 nTPM
PBMC	23.9 nTPM
Adipose tissue	12.5 nTPM
Breast	11.3 nTPM

Endothelial cells	10.2 nTPM
Prostate	20.5 nTPM
Lung	15.6 nTPM
Skin	15.5 nTPM
Ovary	15.4 nTPM
Endometrium	14.9 nTPM
Skeletal muscle	12.0 nTPM
Pancreas	7.7 nTPM
Esophagus	7.3 nTPM
Heart muscle	6.6 nTPM
Breast	6.5 nTPM
Liver	6.4 nTPM
Placenta	6.2 nTPM
Eye	4.8 nTPM
Testis	3.5 nTPM

Fibroblasts	11.0 nTPM
Stomach	19.3 nTPM
Ovary	16.4 nTPM
Prostate	15.8 nTPM
Lung	15.7 nTPM
Skin	13.2 nTPM
Skeletal muscle	11.4 nTPM
Heart muscle	8.6 nTPM
Placenta	7.7 nTPM
Adipose tissue	5.3 nTPM
Breast	5.2 nTPM
Esophagus	2.6 nTPM

Intestinal goblet cells	12.0 nTPM
Small intestine	13.3 nTPM
Rectum	12.0 nTPM
Colon	10.7 nTPM

Macrophages	19.0 nTPM
Adipose tissue	45.3 nTPM
Prostate	32.0 nTPM
Skeletal muscle	29.9 nTPM
Endometrium	25.3 nTPM
Spleen	22.8 nTPM
Pancreas	20.4 nTPM
Ovary	15.9 nTPM
Bone marrow	15.0 nTPM
Lung	14.8 nTPM
PBMC	13.6 nTPM
Breast	12.8 nTPM
Esophagus	12.6 nTPM
Stomach	11.7 nTPM
Kidney	7.6 nTPM
Testis	5.8 nTPM

Paneth cells	28.6 nTPM
Colon	59.3 nTPM
Small intestine	15.0 nTPM
Rectum	11.6 nTPM

Plasma cells	8.6 nTPM
Bone marrow	13.2 nTPM
Adipose tissue	12.2 nTPM
Spleen	12.0 nTPM
Stomach	5.4 nTPM
Esophagus	0.0 nTPM

Respiratory ciliated cells	7.6 nTPM
Bronchus	7.9 nTPM
Lung	7.3 nTPM

Smooth muscle cells	10.3 nTPM
Ovary	26.3 nTPM
Skin	19.2 nTPM
Endometrium	12.7 nTPM
Esophagus	9.3 nTPM
Prostate	9.1 nTPM
Skeletal muscle	8.9 nTPM
Adipose tissue	8.3 nTPM
Pancreas	6.8 nTPM
Heart muscle	5.7 nTPM
Breast	4.5 nTPM
Bronchus	2.0 nTPM

T-cells	6.3 nTPM
Endometrium	16.4 nTPM
Skeletal muscle	11.2 nTPM
Colon	10.9 nTPM
Skin	10.2 nTPM
Breast	9.3 nTPM
Lymph node	7.5 nTPM
Adipose tissue	7.3 nTPM
Liver	6.9 nTPM
Spleen	4.8 nTPM
PBMC	4.6 nTPM
Ovary	4.5 nTPM
Stomach	3.8 nTPM
Bone marrow	3.7 nTPM
Lung	3.0 nTPM
Kidney	2.7 nTPM
Prostate	0.0 nTPM
Esophagus	0.0 nTPM

Basal keratinocytes	17.7 nTPM
Skin	17.7 nTPM

Basal prostatic cells	14.7 nTPM
Prostate	14.7 nTPM

Basal respiratory cells	4.7 nTPM
Bronchus	4.7 nTPM

Collecting duct cells	9.2 nTPM
Kidney	9.2 nTPM

Undifferentiated cells	15.9 nTPM
Colon	19.4 nTPM
Small intestine	16.4 nTPM
Rectum	12.1 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC