Single cell type - MADD - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	MADD
Gene descriptionⁱ Full gene name according to HGNC.	MAP kinase activating death domain
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Human disease related genes
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	15

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Horizontal cells - Visual perception (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Horizontal cells, Cone photoreceptor cells, Rod photoreceptor cells, Bipolar cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enriched (basophil)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Horizontal cells, Cone photoreceptor cells, Rod photoreceptor cells, Bipolar cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

MADD is part of cluster 62 Horizontal cells - Visual perception with confidenceⁱ 1
204 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
SLC4A5	Solute carrier family 4 member 5	0.6736	62
GJD2	Gap junction protein delta 2	0.6639	9
CELSR3	Cadherin EGF LAG seven-pass G-type receptor 3	0.6290	62
RNF165	Ring finger protein 165	0.6243	62
FAM222A	Family with sequence similarity 222 member A	0.5991	46
KIF1A	Kinesin family member 1A	0.5766	62
RPS6KL1	Ribosomal protein S6 kinase like 1	0.5745	9
NPAS4	Neuronal PAS domain protein 4	0.5679	62
SRRM3	Serine/arginine repetitive matrix 3	0.5651	9
SCRT2	Scratch family transcriptional repressor 2	0.5609	62
TADA2B	Transcriptional adaptor 2B	0.5573	46
DRD2	Dopamine receptor D2	0.5565	62
GRIPAP1	GRIP1 associated protein 1	0.5559	9
KMT2D	Lysine methyltransferase 2D	0.5540	9
PTPRN	Protein tyrosine phosphatase receptor type N	0.5508	62

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	10.6 nTPM
Breast	11.9 nTPM
Adipose tissue	9.2 nTPM

Alveolar cells type 1	12.8 nTPM
Lung	12.8 nTPM

Alveolar cells type 2	9.2 nTPM
Lung	9.2 nTPM

Astrocytes	21.5 nTPM
Brain	21.5 nTPM

B-cells	11.7 nTPM
Adipose tissue	16.1 nTPM
Spleen	14.2 nTPM
Lymph node	14.1 nTPM
Bone marrow	12.3 nTPM
PBMC	11.5 nTPM
Stomach	11.3 nTPM
Breast	10.7 nTPM
Colon	9.6 nTPM
Kidney	9.5 nTPM
Liver	7.2 nTPM

Dendritic cells	29.0 nTPM
Adipose tissue	56.7 nTPM
Breast	18.2 nTPM
PBMC	12.0 nTPM

Endothelial cells	11.5 nTPM
Eye	16.2 nTPM
Heart muscle	16.2 nTPM
Testis	13.6 nTPM
Ovary	13.2 nTPM
Liver	12.8 nTPM
Lung	12.5 nTPM
Breast	11.9 nTPM
Endometrium	11.3 nTPM
Skeletal muscle	11.0 nTPM
Skin	10.3 nTPM
Esophagus	9.7 nTPM
Prostate	9.2 nTPM
Placenta	8.6 nTPM
Pancreas	3.9 nTPM

Fibroblasts	7.8 nTPM
Heart muscle	13.7 nTPM
Ovary	10.0 nTPM
Esophagus	9.7 nTPM
Skin	8.5 nTPM
Skeletal muscle	8.4 nTPM
Prostate	6.5 nTPM
Adipose tissue	6.3 nTPM
Stomach	6.0 nTPM
Breast	5.7 nTPM
Lung	5.6 nTPM
Placenta	5.5 nTPM

Intestinal goblet cells	17.7 nTPM
Small intestine	20.3 nTPM
Rectum	20.0 nTPM
Colon	12.9 nTPM

Macrophages	14.9 nTPM
PBMC	27.3 nTPM
Ovary	19.0 nTPM
Lung	18.8 nTPM
Adipose tissue	17.6 nTPM
Stomach	17.6 nTPM
Esophagus	17.3 nTPM
Spleen	16.5 nTPM
Skeletal muscle	15.9 nTPM
Breast	13.9 nTPM
Bone marrow	12.5 nTPM
Endometrium	12.3 nTPM
Kidney	12.0 nTPM
Testis	9.8 nTPM
Pancreas	7.7 nTPM
Prostate	4.6 nTPM

Paneth cells	12.2 nTPM
Rectum	15.3 nTPM
Small intestine	11.3 nTPM
Colon	9.9 nTPM

Plasma cells	17.7 nTPM
Adipose tissue	56.1 nTPM
Esophagus	12.3 nTPM
Stomach	8.3 nTPM
Spleen	6.9 nTPM
Bone marrow	5.0 nTPM

Respiratory ciliated cells	6.2 nTPM
Bronchus	11.0 nTPM
Lung	1.5 nTPM

Smooth muscle cells	7.7 nTPM
Heart muscle	15.6 nTPM
Adipose tissue	13.3 nTPM
Ovary	10.2 nTPM
Endometrium	8.4 nTPM
Skin	7.8 nTPM
Skeletal muscle	7.7 nTPM
Breast	6.2 nTPM
Prostate	4.7 nTPM
Pancreas	4.1 nTPM
Bronchus	3.7 nTPM
Esophagus	3.1 nTPM

T-cells	15.4 nTPM
Adipose tissue	31.6 nTPM
Skeletal muscle	24.1 nTPM
Lymph node	20.0 nTPM
Esophagus	19.7 nTPM
Endometrium	19.6 nTPM
Lung	18.2 nTPM
Breast	17.1 nTPM
Bone marrow	16.7 nTPM
Spleen	16.2 nTPM
Colon	15.7 nTPM
Stomach	14.4 nTPM
PBMC	13.5 nTPM
Skin	11.6 nTPM
Liver	11.2 nTPM
Ovary	6.7 nTPM
Kidney	5.5 nTPM
Prostate	0.0 nTPM

Basal respiratory cells	6.1 nTPM
Bronchus	6.1 nTPM

Basal squamous epithelial cells	6.8 nTPM
Esophagus	6.8 nTPM

Collecting duct cells	7.3 nTPM
Kidney	7.3 nTPM

Distal enterocytes	21.2 nTPM
Rectum	26.5 nTPM
Colon	15.8 nTPM

Undifferentiated cells	13.7 nTPM
Colon	17.2 nTPM
Rectum	13.8 nTPM
Small intestine	10.2 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC