Single cell type - ACO1 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ACO1
Gene descriptionⁱ Full gene name according to HGNC.	Aconitase 1
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Cancer-related genes Citric acid cycle related proteins Enzymes Metabolic proteins Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	3

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Proximal tubular cells - Tubular reabsorption (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Hepatocytes, Proximal tubular cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (ASC diff)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Hepatocytes, Proximal tubular cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

ACO1 is part of cluster 67 Proximal tubular cells - Tubular reabsorption with confidenceⁱ 1
499 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
SLC27A2	Solute carrier family 27 member 2	0.7296	67
SHMT1	Serine hydroxymethyltransferase 1	0.7062	67
QPRT	Quinolinate phosphoribosyltransferase	0.6953	67
DPYS	Dihydropyrimidinase	0.6920	67
APOM	Apolipoprotein M	0.6744	67
GLTPD2	Glycolipid transfer protein domain containing 2	0.6718	67
SLC16A4	Solute carrier family 16 member 4	0.6703	67
BHMT	Betaine--homocysteine S-methyltransferase	0.6691	67
SUOX	Sulfite oxidase	0.6684	67
PRODH2	Proline dehydrogenase 2	0.6610	67
ALDH8A1	Aldehyde dehydrogenase 8 family member A1	0.6546	67
AGXT2	Alanine--glyoxylate aminotransferase 2	0.6545	67
HPD	4-hydroxyphenylpyruvate dioxygenase	0.6535	67
SLC22A12	Solute carrier family 22 member 12	0.6530	67
NAT8	N-acetyltransferase 8 (putative)	0.6523	67

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	16.3 nTPM
Breast	16.8 nTPM
Adipose tissue	15.7 nTPM

Alveolar cells type 1	3.2 nTPM
Lung	3.2 nTPM

Alveolar cells type 2	25.7 nTPM
Lung	25.7 nTPM

Astrocytes	43.8 nTPM
Brain	43.8 nTPM

B-cells	6.7 nTPM
Liver	12.7 nTPM
Stomach	10.0 nTPM
Kidney	9.5 nTPM
PBMC	7.1 nTPM
Colon	6.4 nTPM
Bone marrow	4.9 nTPM
Lymph node	4.4 nTPM
Adipose tissue	4.0 nTPM
Breast	4.0 nTPM
Spleen	3.8 nTPM

Dendritic cells	6.2 nTPM
Adipose tissue	8.2 nTPM
Breast	6.5 nTPM
PBMC	4.0 nTPM

Endothelial cells	18.7 nTPM
Lung	49.9 nTPM
Liver	23.8 nTPM
Skeletal muscle	23.8 nTPM
Prostate	21.4 nTPM
Heart muscle	20.1 nTPM
Testis	17.7 nTPM
Ovary	16.1 nTPM
Endometrium	16.0 nTPM
Eye	15.4 nTPM
Placenta	12.5 nTPM
Breast	12.1 nTPM
Pancreas	11.6 nTPM
Skin	11.2 nTPM
Esophagus	10.8 nTPM

Fibroblasts	29.7 nTPM
Prostate	45.4 nTPM
Skeletal muscle	43.0 nTPM
Adipose tissue	34.9 nTPM
Stomach	32.7 nTPM
Heart muscle	29.4 nTPM
Skin	26.7 nTPM
Breast	25.3 nTPM
Ovary	24.2 nTPM
Esophagus	23.5 nTPM
Lung	22.4 nTPM
Placenta	18.9 nTPM

Intestinal goblet cells	27.7 nTPM
Rectum	36.7 nTPM
Small intestine	32.1 nTPM
Colon	14.3 nTPM

Macrophages	15.6 nTPM
Pancreas	48.5 nTPM
Lung	47.2 nTPM
Ovary	25.4 nTPM
Kidney	24.2 nTPM
PBMC	13.6 nTPM
Skeletal muscle	11.4 nTPM
Spleen	11.4 nTPM
Esophagus	9.4 nTPM
Breast	8.2 nTPM
Adipose tissue	7.3 nTPM
Endometrium	6.8 nTPM
Stomach	5.9 nTPM
Testis	5.8 nTPM
Prostate	5.7 nTPM
Bone marrow	3.7 nTPM

Paneth cells	23.7 nTPM
Rectum	25.6 nTPM
Colon	23.1 nTPM
Small intestine	22.5 nTPM

Plasma cells	7.2 nTPM
Esophagus	12.3 nTPM
Stomach	8.4 nTPM
Bone marrow	6.6 nTPM
Adipose tissue	4.5 nTPM
Spleen	4.0 nTPM

Respiratory ciliated cells	10.7 nTPM
Bronchus	12.8 nTPM
Lung	8.7 nTPM

Smooth muscle cells	22.2 nTPM
Pancreas	40.5 nTPM
Prostate	28.8 nTPM
Skeletal muscle	28.2 nTPM
Adipose tissue	28.2 nTPM
Heart muscle	24.5 nTPM
Endometrium	22.5 nTPM
Breast	21.6 nTPM
Ovary	18.0 nTPM
Skin	17.5 nTPM
Esophagus	12.4 nTPM
Bronchus	1.9 nTPM

T-cells	7.5 nTPM
Kidney	19.1 nTPM
Liver	12.0 nTPM
PBMC	10.9 nTPM
Prostate	9.5 nTPM
Endometrium	8.2 nTPM
Breast	7.5 nTPM
Stomach	7.1 nTPM
Esophagus	6.6 nTPM
Bone marrow	6.6 nTPM
Lung	6.1 nTPM
Adipose tissue	6.0 nTPM
Skeletal muscle	5.9 nTPM
Colon	5.7 nTPM
Skin	5.0 nTPM
Spleen	4.8 nTPM
Lymph node	3.7 nTPM
Ovary	2.2 nTPM

Basal prostatic cells	7.9 nTPM
Prostate	7.9 nTPM

Basal respiratory cells	11.9 nTPM
Bronchus	11.9 nTPM

Basal squamous epithelial cells	9.1 nTPM
Esophagus	9.1 nTPM

Cholangiocytes	38.6 nTPM
Liver	38.6 nTPM

Undifferentiated cells	34.6 nTPM
Small intestine	46.3 nTPM
Rectum	35.4 nTPM
Colon	22.1 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC