Single cell type - RTN2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	RTN2
Gene descriptionⁱ Full gene name according to HGNC.	Reticulon 2
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Human disease related genes
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	4

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Extravillous trophoblasts - Unknown function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Group enriched (Extravillous trophoblasts, Cytotrophoblasts, Syncytiotrophoblasts, Skeletal myocytes)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Group enriched (basophil, eosinophil, plasmacytoid DC)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in some
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (RH-30, RT4)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Group enriched (Extravillous trophoblasts, Cytotrophoblasts, Syncytiotrophoblasts, Skeletal myocytes)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

RTN2 is part of cluster 38 Extravillous trophoblasts - Unknown function with confidenceⁱ 1
176 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
SKP2	S-phase kinase associated protein 2	0.7611	38
ISM2	Isthmin 2	0.7607	38
XAGE2	X antigen family member 2	0.7580	38
ERVH48-1	Endogenous retrovirus group 48 member 1	0.7568	38
EZHIP	EZH inhibitory protein	0.7506	38
AC091959.1	Novel readthrough transcript	0.7361	38
ERVMER34-1	Endogenous retrovirus group MER34 member 1, envelope	0.7324	38
POLR1G	RNA polymerase I subunit G	0.7185	38
GJA5	Gap junction protein alpha 5	0.7142	38
SLC29A1	Solute carrier family 29 member 1 (Augustine blood group)	0.6992	38
PEDS1	Plasmanylethanolamine desaturase 1	0.6991	38
CLDN6	Claudin 6	0.6860	38
DLX3	Distal-less homeobox 3	0.6807	38
ACKR2	Atypical chemokine receptor 2	0.6717	38
HROB	Homologous recombination factor with OB-fold	0.6684	38

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	9.8 nTPM
Breast	12.9 nTPM
Adipose tissue	6.8 nTPM

Alveolar cells type 1	3.2 nTPM
Lung	3.2 nTPM

Alveolar cells type 2	8.1 nTPM
Lung	8.1 nTPM

Astrocytes	2.6 nTPM
Brain	2.6 nTPM

B-cells	0.8 nTPM
Breast	4.0 nTPM
Liver	2.0 nTPM
Spleen	0.7 nTPM
Lymph node	0.6 nTPM
Stomach	0.5 nTPM
Colon	0.0 nTPM
Kidney	0.0 nTPM
PBMC	0.0 nTPM
Adipose tissue	0.0 nTPM
Bone marrow	0.0 nTPM

Dendritic cells	2.9 nTPM
Breast	4.3 nTPM
PBMC	4.0 nTPM
Adipose tissue	0.4 nTPM

Endothelial cells	15.3 nTPM
Placenta	43.8 nTPM
Liver	37.6 nTPM
Testis	22.2 nTPM
Ovary	20.3 nTPM
Skin	15.5 nTPM
Esophagus	13.7 nTPM
Breast	13.7 nTPM
Pancreas	11.6 nTPM
Prostate	9.9 nTPM
Endometrium	8.9 nTPM
Eye	8.1 nTPM
Skeletal muscle	7.5 nTPM
Heart muscle	0.9 nTPM
Lung	0.0 nTPM

Fibroblasts	14.0 nTPM
Placenta	43.0 nTPM
Adipose tissue	23.0 nTPM
Esophagus	20.2 nTPM
Skeletal muscle	14.8 nTPM
Stomach	13.4 nTPM
Breast	12.4 nTPM
Ovary	10.0 nTPM
Prostate	8.6 nTPM
Skin	8.0 nTPM
Heart muscle	0.9 nTPM
Lung	0.0 nTPM

Intestinal goblet cells	2.2 nTPM
Colon	3.6 nTPM
Rectum	2.6 nTPM
Small intestine	0.4 nTPM

Macrophages	10.7 nTPM
PBMC	40.9 nTPM
Pancreas	20.4 nTPM
Ovary	17.9 nTPM
Testis	14.5 nTPM
Prostate	12.6 nTPM
Breast	11.7 nTPM
Esophagus	7.9 nTPM
Skeletal muscle	6.5 nTPM
Adipose tissue	6.3 nTPM
Endometrium	6.2 nTPM
Lung	5.4 nTPM
Bone marrow	3.7 nTPM
Stomach	2.9 nTPM
Kidney	2.3 nTPM
Spleen	2.1 nTPM

Paneth cells	0.3 nTPM
Rectum	1.0 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM

Plasma cells	2.5 nTPM
Esophagus	6.1 nTPM
Stomach	5.7 nTPM
Adipose tissue	0.6 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM

Respiratory ciliated cells	1.3 nTPM
Lung	1.5 nTPM
Bronchus	1.0 nTPM

Smooth muscle cells	12.3 nTPM
Esophagus	18.6 nTPM
Breast	18.0 nTPM
Pancreas	17.8 nTPM
Skeletal muscle	15.9 nTPM
Ovary	15.8 nTPM
Adipose tissue	14.3 nTPM
Prostate	11.2 nTPM
Skin	11.1 nTPM
Endometrium	9.1 nTPM
Bronchus	1.8 nTPM
Heart muscle	1.0 nTPM

T-cells	1.0 nTPM
Ovary	4.5 nTPM
Skin	2.8 nTPM
Skeletal muscle	2.8 nTPM
Liver	1.5 nTPM
Lung	1.5 nTPM
Breast	1.5 nTPM
Endometrium	1.3 nTPM
Lymph node	0.5 nTPM
Spleen	0.5 nTPM
Adipose tissue	0.3 nTPM
Bone marrow	0.3 nTPM
PBMC	0.2 nTPM
Colon	0.0 nTPM
Kidney	0.0 nTPM
Prostate	0.0 nTPM
Esophagus	0.0 nTPM
Stomach	0.0 nTPM

Basal keratinocytes	6.4 nTPM
Skin	6.4 nTPM

Basal prostatic cells	27.7 nTPM
Prostate	27.7 nTPM

Cytotrophoblasts	134.3 nTPM
Placenta	134.3 nTPM

Endometrial stromal cells	7.0 nTPM
Endometrium	7.0 nTPM

Undifferentiated cells	0.7 nTPM
Colon	0.9 nTPM
Rectum	0.9 nTPM
Small intestine	0.3 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC