Single cell type - ASL - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ASL
Gene descriptionⁱ Full gene name according to HGNC.	Argininosuccinate lyase
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Enzymes Human disease related genes Metabolic proteins Plasma proteins Potential drug targets
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	8

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Intestinal epithelial cells - Unknown function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Hepatocytes, Distal enterocytes, Proximal enterocytes, Proximal tubular cells, Paneth cells, Exocrine glandular cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Hepatocytes, Distal enterocytes, Proximal enterocytes, Proximal tubular cells, Paneth cells, Exocrine glandular cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

ASL is part of cluster 53 Intestinal epithelial cells - Unknown function with confidenceⁱ 1
611 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
GPT	Glutamic--pyruvic transaminase	0.7741	53
CACFD1	Calcium channel flower domain containing 1	0.6584	53
UGT2B17	UDP glucuronosyltransferase family 2 member B17	0.6295	53
HNF4A	Hepatocyte nuclear factor 4 alpha	0.6162	53
FP565260.2	Novel protein	0.6116	53
MVD	Mevalonate diphosphate decarboxylase	0.6070	15
ZNHIT1	Zinc finger HIT-type containing 1	0.5992	67
CC2D1A	Coiled-coil and C2 domain containing 1A	0.5991	53
CISD3	CDGSH iron sulfur domain 3	0.5969	45
EIF3J	Eukaryotic translation initiation factor 3 subunit J	0.5946	39
AGXT	Alanine--glyoxylate and serine--pyruvate aminotransferase	0.5941	2
LRG1	Leucine rich alpha-2-glycoprotein 1	0.5937	15
PKP3	Plakophilin 3	0.5897	53
LDHD	Lactate dehydrogenase D	0.5883	53
NR1I3	Nuclear receptor subfamily 1 group I member 3	0.5882	67

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	34.5 nTPM
Breast	36.3 nTPM
Adipose tissue	32.8 nTPM

Alveolar cells type 1	19.2 nTPM
Lung	19.2 nTPM

Alveolar cells type 2	61.4 nTPM
Lung	61.4 nTPM

Astrocytes	5.2 nTPM
Brain	5.2 nTPM

B-cells	20.4 nTPM
Liver	53.1 nTPM
Colon	25.5 nTPM
PBMC	20.0 nTPM
Kidney	19.0 nTPM
Bone marrow	16.4 nTPM
Breast	16.0 nTPM
Stomach	15.9 nTPM
Spleen	14.9 nTPM
Lymph node	12.8 nTPM
Adipose tissue	10.0 nTPM

Dendritic cells	25.4 nTPM
Adipose tissue	43.9 nTPM
Breast	28.4 nTPM
PBMC	4.0 nTPM

Endothelial cells	30.7 nTPM
Pancreas	50.2 nTPM
Liver	46.7 nTPM
Ovary	39.1 nTPM
Esophagus	34.1 nTPM
Breast	32.0 nTPM
Placenta	31.8 nTPM
Skeletal muscle	31.7 nTPM
Eye	28.3 nTPM
Testis	27.9 nTPM
Skin	27.6 nTPM
Prostate	22.8 nTPM
Lung	21.8 nTPM
Endometrium	18.9 nTPM
Heart muscle	17.2 nTPM

Fibroblasts	14.4 nTPM
Placenta	27.4 nTPM
Stomach	20.8 nTPM
Prostate	16.5 nTPM
Adipose tissue	14.9 nTPM
Skin	14.3 nTPM
Breast	14.3 nTPM
Ovary	13.0 nTPM
Skeletal muscle	12.5 nTPM
Esophagus	12.1 nTPM
Heart muscle	6.8 nTPM
Lung	5.6 nTPM

Intestinal goblet cells	126.5 nTPM
Colon	196.5 nTPM
Small intestine	110.4 nTPM
Rectum	72.7 nTPM

Macrophages	22.3 nTPM
Kidney	40.1 nTPM
Stomach	35.1 nTPM
Bone marrow	30.0 nTPM
Breast	28.7 nTPM
Pancreas	23.0 nTPM
Adipose tissue	22.8 nTPM
Endometrium	22.5 nTPM
Skeletal muscle	19.9 nTPM
Spleen	19.7 nTPM
Lung	18.2 nTPM
Prostate	17.2 nTPM
Ovary	16.3 nTPM
Esophagus	15.8 nTPM
PBMC	13.6 nTPM
Testis	11.7 nTPM

Paneth cells	240.4 nTPM
Colon	342.6 nTPM
Small intestine	232.7 nTPM
Rectum	145.9 nTPM

Plasma cells	23.4 nTPM
Adipose tissue	34.2 nTPM
Spleen	24.3 nTPM
Bone marrow	21.4 nTPM
Stomach	18.5 nTPM
Esophagus	18.4 nTPM

Respiratory ciliated cells	42.8 nTPM
Bronchus	43.4 nTPM
Lung	42.3 nTPM

Smooth muscle cells	17.5 nTPM
Pancreas	49.4 nTPM
Skeletal muscle	18.3 nTPM
Breast	17.1 nTPM
Bronchus	16.0 nTPM
Heart muscle	15.4 nTPM
Ovary	15.2 nTPM
Adipose tissue	14.7 nTPM
Esophagus	12.4 nTPM
Prostate	12.2 nTPM
Skin	12.2 nTPM
Endometrium	9.7 nTPM

T-cells	18.0 nTPM
Liver	45.9 nTPM
Kidney	32.9 nTPM
Colon	30.0 nTPM
Lung	24.3 nTPM
Ovary	20.1 nTPM
Breast	19.4 nTPM
Skeletal muscle	18.9 nTPM
Endometrium	18.1 nTPM
Skin	18.0 nTPM
Adipose tissue	16.4 nTPM
Spleen	14.8 nTPM
PBMC	12.0 nTPM
Bone marrow	11.6 nTPM
Lymph node	9.5 nTPM
Stomach	9.1 nTPM
Prostate	4.7 nTPM
Esophagus	0.0 nTPM

Basal keratinocytes	5.5 nTPM
Skin	5.5 nTPM

Basal respiratory cells	24.2 nTPM
Bronchus	24.2 nTPM

Cholangiocytes	124.4 nTPM
Liver	124.4 nTPM

Collecting duct cells	31.3 nTPM
Kidney	31.3 nTPM

Undifferentiated cells	189.4 nTPM
Colon	308.3 nTPM
Small intestine	136.1 nTPM
Rectum	124.0 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC