Single cell type - NT5E - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	NT5E
Gene descriptionⁱ Full gene name according to HGNC.	5'-nucleotidase ecto
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	CD markers Disease related genes Enzymes Human disease related genes Metabolic proteins Potential drug targets
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	5

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Fibroblasts - ECM organization (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Pancreatic endocrine cells, Rod photoreceptor cells, Oligodendrocyte precursor cells, Glandular and luminal cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enhanced (naive B-cell, naive CD8 T-cell)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (hTERT-RPE1, LHCN-M2, TIME, U-87 MG)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Pancreatic endocrine cells, Rod photoreceptor cells, Oligodendrocyte precursor cells, Glandular and luminal cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

NT5E is part of cluster 50 Fibroblasts - ECM organization with confidenceⁱ 1
441 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
LRRN4CL	LRRN4 C-terminal like	0.6554	50
PI16	Peptidase inhibitor 16	0.6350	50
IGFBP6	Insulin like growth factor binding protein 6	0.6289	50
PTGIS	Prostaglandin I2 synthase	0.6210	50
C17orf58	Chromosome 17 open reading frame 58	0.6182	50
OLFML1	Olfactomedin like 1	0.6164	50
CHRDL1	Chordin like 1	0.6085	50
SCARA5	Scavenger receptor class A member 5	0.6000	50
H3Y1	H3.Y histone 1	0.5974	50
ABI3BP	ABI family member 3 binding protein	0.5910	50
PRELP	Proline and arginine rich end leucine rich repeat protein	0.5902	50
MMP27	Matrix metallopeptidase 27	0.5871	50
F10	Coagulation factor X	0.5869	50
MMP2	Matrix metallopeptidase 2	0.5780	50
CD248	CD248 molecule	0.5779	50

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	6.4 nTPM
Adipose tissue	9.3 nTPM
Breast	3.6 nTPM

Alveolar cells type 1	0.0 nTPM
Lung	0.0 nTPM

Alveolar cells type 2	5.8 nTPM
Lung	5.8 nTPM

Astrocytes	36.9 nTPM
Brain	36.9 nTPM

B-cells	21.6 nTPM
Spleen	36.0 nTPM
Bone marrow	30.2 nTPM
Lymph node	30.0 nTPM
Stomach	26.8 nTPM
PBMC	22.8 nTPM
Adipose tissue	20.6 nTPM
Kidney	19.0 nTPM
Colon	14.4 nTPM
Breast	8.1 nTPM
Liver	7.6 nTPM

Dendritic cells	2.2 nTPM
PBMC	4.0 nTPM
Breast	1.8 nTPM
Adipose tissue	0.7 nTPM

Endothelial cells	20.5 nTPM
Pancreas	57.9 nTPM
Placenta	57.4 nTPM
Esophagus	30.6 nTPM
Ovary	24.6 nTPM
Skin	24.5 nTPM
Lung	21.8 nTPM
Liver	19.2 nTPM
Breast	11.0 nTPM
Prostate	10.1 nTPM
Heart muscle	8.8 nTPM
Endometrium	7.0 nTPM
Skeletal muscle	6.2 nTPM
Eye	4.1 nTPM
Testis	4.1 nTPM

Fibroblasts	39.4 nTPM
Adipose tissue	107.9 nTPM
Esophagus	77.2 nTPM
Skeletal muscle	73.0 nTPM
Skin	55.3 nTPM
Stomach	37.1 nTPM
Breast	30.6 nTPM
Prostate	20.5 nTPM
Heart muscle	18.0 nTPM
Lung	10.1 nTPM
Placenta	3.0 nTPM
Ovary	0.6 nTPM

Intestinal goblet cells	27.7 nTPM
Rectum	39.6 nTPM
Small intestine	30.7 nTPM
Colon	12.9 nTPM

Macrophages	2.1 nTPM
Pancreas	12.8 nTPM
Skeletal muscle	5.9 nTPM
Endometrium	5.8 nTPM
Kidney	3.0 nTPM
Lung	1.8 nTPM
Prostate	1.2 nTPM
Breast	0.7 nTPM
Adipose tissue	0.5 nTPM
Ovary	0.1 nTPM
PBMC	0.0 nTPM
Testis	0.0 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM
Stomach	0.0 nTPM

Paneth cells	46.0 nTPM
Rectum	71.8 nTPM
Colon	36.2 nTPM
Small intestine	30.0 nTPM

Plasma cells	3.6 nTPM
Esophagus	12.3 nTPM
Stomach	4.6 nTPM
Adipose tissue	0.6 nTPM
Spleen	0.6 nTPM
Bone marrow	0.0 nTPM

Respiratory ciliated cells	4.8 nTPM
Lung	7.3 nTPM
Bronchus	2.2 nTPM

Smooth muscle cells	9.6 nTPM
Pancreas	55.4 nTPM
Skin	9.1 nTPM
Adipose tissue	8.3 nTPM
Heart muscle	8.0 nTPM
Prostate	5.3 nTPM
Endometrium	5.2 nTPM
Esophagus	4.6 nTPM
Breast	3.9 nTPM
Bronchus	2.9 nTPM
Skeletal muscle	2.4 nTPM
Ovary	0.1 nTPM

T-cells	5.0 nTPM
Esophagus	13.1 nTPM
Lymph node	11.0 nTPM
Skin	7.8 nTPM
Endometrium	7.8 nTPM
Colon	7.1 nTPM
Stomach	7.0 nTPM
Lung	6.1 nTPM
PBMC	5.6 nTPM
Spleen	4.7 nTPM
Skeletal muscle	3.8 nTPM
Kidney	3.4 nTPM
Bone marrow	2.7 nTPM
Breast	2.1 nTPM
Liver	2.0 nTPM
Adipose tissue	1.5 nTPM
Prostate	0.0 nTPM
Ovary	0.0 nTPM

Basal prostatic cells	1.7 nTPM
Prostate	1.7 nTPM

Basal squamous epithelial cells	2.8 nTPM
Esophagus	2.8 nTPM

Cholangiocytes	27.1 nTPM
Liver	27.1 nTPM

Distal enterocytes	49.2 nTPM
Rectum	81.2 nTPM
Colon	17.2 nTPM

Undifferentiated cells	18.0 nTPM
Rectum	37.0 nTPM
Colon	13.0 nTPM
Small intestine	4.0 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC