Single cell type - CHST12 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	CHST12
Gene descriptionⁱ Full gene name according to HGNC.	Carbohydrate sulfotransferase 12
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes Metabolic proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	3

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	NK-cells & T-cells - Immune response (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (NK-cells, dendritic cells, Hepatic stellate cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (HMC-1, NB-4, U-266/70)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (NK-cells, dendritic cells, Hepatic stellate cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

CHST12 is part of cluster 63 NK-cells & T-cells - Immune response with confidenceⁱ 1
362 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
LIM2	Lens intrinsic membrane protein 2	0.8524	63
TXK	TXK tyrosine kinase	0.8468	63
FGFBP2	Fibroblast growth factor binding protein 2	0.8450	63
KLRF1	Killer cell lectin like receptor F1	0.8427	63
TRDC	T cell receptor delta constant	0.8425	63
CD160	CD160 molecule	0.8342	63
NKG7	Natural killer cell granule protein 7	0.8285	63
CMC1	C-X9-C motif containing 1	0.8140	63
NCR1	Natural cytotoxicity triggering receptor 1	0.8042	63
PLEKHF1	Pleckstrin homology and FYVE domain containing 1	0.8019	63
SH2D1B	SH2 domain containing 1B	0.8019	63
PTGDR	Prostaglandin D2 receptor	0.7993	63
KLRD1	Killer cell lectin like receptor D1	0.7972	63
MATK	Megakaryocyte-associated tyrosine kinase	0.7936	63
KLRB1	Killer cell lectin like receptor B1	0.7927	63

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	36.8 nTPM
Breast	41.0 nTPM
Adipose tissue	32.6 nTPM

Alveolar cells type 1	16.0 nTPM
Lung	16.0 nTPM

Alveolar cells type 2	50.5 nTPM
Lung	50.5 nTPM

Astrocytes	9.1 nTPM
Brain	9.1 nTPM

B-cells	32.8 nTPM
Liver	101.3 nTPM
PBMC	47.1 nTPM
Breast	37.5 nTPM
Kidney	28.5 nTPM
Spleen	22.2 nTPM
Lymph node	20.0 nTPM
Stomach	19.1 nTPM
Adipose tissue	18.6 nTPM
Bone marrow	18.0 nTPM
Colon	16.0 nTPM

Dendritic cells	158.7 nTPM
Adipose tissue	204.1 nTPM
PBMC	151.4 nTPM
Breast	120.7 nTPM

Endothelial cells	59.4 nTPM
Liver	132.1 nTPM
Placenta	87.5 nTPM
Testis	83.9 nTPM
Eye	70.4 nTPM
Pancreas	65.6 nTPM
Breast	56.2 nTPM
Esophagus	54.9 nTPM
Ovary	49.7 nTPM
Skeletal muscle	49.0 nTPM
Skin	45.5 nTPM
Endometrium	40.6 nTPM
Prostate	39.1 nTPM
Lung	37.4 nTPM
Heart muscle	20.3 nTPM

Fibroblasts	37.0 nTPM
Adipose tissue	62.8 nTPM
Skin	56.6 nTPM
Placenta	55.2 nTPM
Breast	43.8 nTPM
Skeletal muscle	41.5 nTPM
Prostate	35.9 nTPM
Esophagus	28.4 nTPM
Lung	27.9 nTPM
Stomach	22.3 nTPM
Ovary	20.9 nTPM
Heart muscle	11.9 nTPM

Intestinal goblet cells	9.1 nTPM
Small intestine	11.3 nTPM
Rectum	10.8 nTPM
Colon	5.0 nTPM

Macrophages	21.2 nTPM
PBMC	68.2 nTPM
Lung	37.4 nTPM
Bone marrow	32.4 nTPM
Testis	23.2 nTPM
Breast	22.3 nTPM
Pancreas	20.4 nTPM
Esophagus	15.8 nTPM
Ovary	15.6 nTPM
Prostate	13.8 nTPM
Kidney	13.7 nTPM
Adipose tissue	13.5 nTPM
Skeletal muscle	13.1 nTPM
Endometrium	10.3 nTPM
Spleen	9.3 nTPM
Stomach	8.8 nTPM

Paneth cells	6.7 nTPM
Rectum	12.6 nTPM
Small intestine	7.5 nTPM
Colon	0.0 nTPM

Plasma cells	107.8 nTPM
Adipose tissue	138.1 nTPM
Stomach	129.4 nTPM
Spleen	113.1 nTPM
Bone marrow	90.6 nTPM
Esophagus	67.8 nTPM

Respiratory ciliated cells	15.2 nTPM
Lung	19.0 nTPM
Bronchus	11.4 nTPM

Smooth muscle cells	17.9 nTPM
Pancreas	36.2 nTPM
Prostate	24.4 nTPM
Breast	23.3 nTPM
Skin	20.6 nTPM
Adipose tissue	19.1 nTPM
Ovary	17.1 nTPM
Skeletal muscle	16.8 nTPM
Esophagus	15.5 nTPM
Endometrium	13.1 nTPM
Bronchus	7.5 nTPM
Heart muscle	3.4 nTPM

T-cells	93.2 nTPM
Liver	212.4 nTPM
Kidney	166.3 nTPM
Ovary	149.0 nTPM
Colon	139.7 nTPM
Skeletal muscle	133.3 nTPM
PBMC	106.3 nTPM
Skin	80.6 nTPM
Bone marrow	79.6 nTPM
Breast	78.3 nTPM
Prostate	75.7 nTPM
Stomach	73.7 nTPM
Endometrium	70.3 nTPM
Adipose tissue	64.4 nTPM
Spleen	55.2 nTPM
Lung	50.1 nTPM
Esophagus	26.2 nTPM
Lymph node	23.8 nTPM

Basal keratinocytes	18.1 nTPM
Skin	18.1 nTPM

Basal prostatic cells	35.5 nTPM
Prostate	35.5 nTPM

Basal respiratory cells	24.3 nTPM
Bronchus	24.3 nTPM

Basal squamous epithelial cells	18.8 nTPM
Esophagus	18.8 nTPM

Undifferentiated cells	12.2 nTPM
Rectum	16.8 nTPM
Small intestine	15.4 nTPM
Colon	4.5 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC