Single cell type - TLR4 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	TLR4
Gene descriptionⁱ Full gene name according to HGNC.	Toll like receptor 4
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	CD markers Enzymes FDA approved drug targets Human disease related genes Transporters
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	3

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Monocytes & Neutrophils - Innate immune response (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Kupffer cells, Macrophages, Hepatic stellate cells, monocytes, Astrocytes, Adipocytes, Hofbauer cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enhanced (neutrophil)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (Karpas-707, TIME)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Kupffer cells, Macrophages, Hepatic stellate cells, monocytes, Astrocytes, Adipocytes, Hofbauer cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

TLR4 is part of cluster 19 Monocytes & Neutrophils - Innate immune response with confidenceⁱ 1
175 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
LILRA6	Leukocyte immunoglobulin like receptor A6	0.7536	19
GIMAP8	GTPase, IMAP family member 8	0.7117	14
CCL24	C-C motif chemokine ligand 24	0.7022	19
TLR8	Toll like receptor 8	0.7006	19
HK3	Hexokinase 3	0.6935	19
LILRB3	Leukocyte immunoglobulin like receptor B3	0.6831	19
NFAM1	NFAT activating protein with ITAM motif 1	0.6827	19
SPIC	Spi-C transcription factor	0.6806	19
LRRC25	Leucine rich repeat containing 25	0.6777	19
GNS	Glucosamine (N-acetyl)-6-sulfatase	0.6707	19
CLEC4D	C-type lectin domain family 4 member D	0.6592	19
CLEC12A	C-type lectin domain family 12 member A	0.6591	19
SLC11A1	Solute carrier family 11 member 1	0.6583	19
P2RY13	Purinergic receptor P2Y13	0.6571	19
NLRC4	NLR family CARD domain containing 4	0.6542	19

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Alveolar cells type 1	0.0 nTPM
Lung	0.0 nTPM

Alveolar cells type 2	5.0 nTPM
Lung	5.0 nTPM

B-cells	2.3 nTPM
Bone marrow	4.9 nTPM
Liver	3.3 nTPM
Colon	3.2 nTPM
PBMC	2.8 nTPM
Breast	2.6 nTPM
Lymph node	2.5 nTPM
Stomach	1.8 nTPM
Spleen	1.4 nTPM
Adipose tissue	1.0 nTPM
Kidney	0.0 nTPM

Basal keratinocytes	0.8 nTPM
Skin	0.8 nTPM

Basal prostatic cells	0.3 nTPM
Prostate	0.3 nTPM

Dendritic cells	0.1 nTPM
Adipose tissue	0.4 nTPM
PBMC	0.0 nTPM
Breast	0.0 nTPM

Endothelial cells	31.6 nTPM
Lung	112.3 nTPM
Liver	85.3 nTPM
Heart muscle	49.3 nTPM
Eye	34.8 nTPM
Skeletal muscle	26.0 nTPM
Breast	22.3 nTPM
Esophagus	22.2 nTPM
Ovary	19.5 nTPM
Endometrium	19.3 nTPM
Testis	17.7 nTPM
Skin	16.2 nTPM
Prostate	8.3 nTPM
Placenta	5.7 nTPM
Pancreas	3.9 nTPM

Fibroblasts	11.5 nTPM
Adipose tissue	25.6 nTPM
Heart muscle	24.4 nTPM
Lung	21.2 nTPM
Esophagus	14.8 nTPM
Skeletal muscle	13.6 nTPM
Stomach	11.9 nTPM
Skin	5.9 nTPM
Breast	5.4 nTPM
Prostate	1.5 nTPM
Ovary	1.4 nTPM
Placenta	0.5 nTPM

Intestinal goblet cells	2.0 nTPM
Colon	5.0 nTPM
Rectum	0.9 nTPM
Small intestine	0.0 nTPM

Macrophages	64.5 nTPM
Ovary	129.6 nTPM
Stomach	123.1 nTPM
Bone marrow	111.2 nTPM
Lung	110.0 nTPM
Adipose tissue	83.5 nTPM
Spleen	80.9 nTPM
Esophagus	61.4 nTPM
Skeletal muscle	60.4 nTPM
Kidney	59.7 nTPM
Testis	46.4 nTPM
Breast	33.1 nTPM
Endometrium	29.8 nTPM
Prostate	25.3 nTPM
Pancreas	12.8 nTPM
PBMC	0.0 nTPM

Paneth cells	6.1 nTPM
Colon	16.5 nTPM
Rectum	1.9 nTPM
Small intestine	0.0 nTPM

Plasma cells	0.1 nTPM
Spleen	0.3 nTPM
Adipose tissue	0.0 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM
Stomach	0.0 nTPM

Respiratory ciliated cells	3.7 nTPM
Bronchus	4.5 nTPM
Lung	2.9 nTPM

Smooth muscle cells	4.5 nTPM
Adipose tissue	20.3 nTPM
Skeletal muscle	11.1 nTPM
Endometrium	4.3 nTPM
Pancreas	4.1 nTPM
Heart muscle	4.0 nTPM
Ovary	2.9 nTPM
Breast	1.6 nTPM
Bronchus	0.9 nTPM
Skin	0.7 nTPM
Prostate	0.4 nTPM
Esophagus	0.0 nTPM

T-cells	0.9 nTPM
Lung	6.1 nTPM
Endometrium	3.9 nTPM
Liver	1.4 nTPM
PBMC	0.9 nTPM
Skin	0.9 nTPM
Skeletal muscle	0.8 nTPM
Breast	0.3 nTPM
Bone marrow	0.2 nTPM
Adipose tissue	0.1 nTPM
Stomach	0.1 nTPM
Colon	0.0 nTPM
Kidney	0.0 nTPM
Prostate	0.0 nTPM
Ovary	0.0 nTPM
Esophagus	0.0 nTPM
Lymph node	0.0 nTPM
Spleen	0.0 nTPM

Astrocytes	50.9 nTPM
Brain	50.9 nTPM

Endometrial stromal cells	29.2 nTPM
Endometrium	29.2 nTPM

Undifferentiated cells	1.2 nTPM
Colon	3.2 nTPM
Rectum	0.2 nTPM
Small intestine	0.1 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC