Single cell type - PIK3R5 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	PIK3R5
Gene descriptionⁱ Full gene name according to HGNC.	Phosphoinositide-3-kinase regulatory subunit 5
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Human disease related genes Metabolic proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	8

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	NK-cells & T-cells - Immune response (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Microglial cells, Langerhans cells, Macrophages, Kupffer cells, NK-cells, dendritic cells, T-cells, Plasma cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (HDLM-2, HMC-1, JURKAT, U-937)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in some
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Microglial cells, Langerhans cells, Macrophages, Kupffer cells, NK-cells, dendritic cells, T-cells, Plasma cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

PIK3R5 is part of cluster 63 NK-cells & T-cells - Immune response with confidenceⁱ 1
362 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
HAVCR2	Hepatitis A virus cellular receptor 2	0.7473	63
CCL4L2	C-C motif chemokine ligand 4 like 2	0.6940	63
CCR5	C-C motif chemokine receptor 5	0.6507	63
AOAH	Acyloxyacyl hydrolase	0.6463	63
GAB3	GRB2 associated binding protein 3	0.6218	63
FMNL1	Formin like 1	0.6162	63
CCL4	C-C motif chemokine ligand 4	0.6112	63
SELPLG	Selectin P ligand	0.5958	65
PREX1	Phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 1	0.5799	41
HCST	Hematopoietic cell signal transducer	0.5779	63
CCL3	C-C motif chemokine ligand 3	0.5772	4
LCP2	Lymphocyte cytosolic protein 2	0.5769	58
PLEK	Pleckstrin	0.5761	51
ARL4C	ADP ribosylation factor like GTPase 4C	0.5730	24
PLCB2	Phospholipase C beta 2	0.5692	19

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	0.2 nTPM
Breast	0.4 nTPM
Adipose tissue	0.0 nTPM

Alveolar cells type 1	0.0 nTPM
Lung	0.0 nTPM

Alveolar cells type 2	6.1 nTPM
Lung	6.1 nTPM

Astrocytes	0.7 nTPM
Brain	0.7 nTPM

B-cells	11.7 nTPM
Adipose tissue	17.6 nTPM
Liver	16.5 nTPM
Stomach	16.4 nTPM
Bone marrow	14.7 nTPM
PBMC	12.9 nTPM
Colon	11.2 nTPM
Lymph node	10.1 nTPM
Spleen	9.7 nTPM
Breast	8.1 nTPM
Kidney	0.0 nTPM

Dendritic cells	29.9 nTPM
Adipose tissue	44.9 nTPM
Breast	32.8 nTPM
PBMC	12.0 nTPM

Endothelial cells	0.7 nTPM
Ovary	2.1 nTPM
Liver	1.8 nTPM
Heart muscle	1.6 nTPM
Skin	1.6 nTPM
Esophagus	1.0 nTPM
Skeletal muscle	0.4 nTPM
Testis	0.3 nTPM
Endometrium	0.3 nTPM
Breast	0.2 nTPM
Eye	0.0 nTPM
Lung	0.0 nTPM
Placenta	0.0 nTPM
Prostate	0.0 nTPM
Pancreas	0.0 nTPM

Fibroblasts	0.5 nTPM
Lung	2.2 nTPM
Skin	1.6 nTPM
Placenta	0.5 nTPM
Breast	0.5 nTPM
Heart muscle	0.3 nTPM
Skeletal muscle	0.1 nTPM
Adipose tissue	0.1 nTPM
Esophagus	0.1 nTPM
Prostate	0.0 nTPM
Ovary	0.0 nTPM
Stomach	0.0 nTPM

Intestinal goblet cells	0.0 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM
Rectum	0.0 nTPM

Macrophages	36.8 nTPM
Adipose tissue	82.4 nTPM
Ovary	66.6 nTPM
Stomach	55.7 nTPM
Esophagus	47.2 nTPM
Prostate	42.3 nTPM
Skeletal muscle	42.0 nTPM
Lung	35.9 nTPM
Endometrium	32.8 nTPM
Spleen	31.1 nTPM
Pancreas	30.7 nTPM
Bone marrow	28.7 nTPM
Testis	25.0 nTPM
Breast	20.4 nTPM
Kidney	11.4 nTPM
PBMC	0.0 nTPM

Paneth cells	0.0 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM
Rectum	0.0 nTPM

Plasma cells	24.7 nTPM
Adipose tissue	34.2 nTPM
Bone marrow	31.3 nTPM
Stomach	28.9 nTPM
Spleen	22.7 nTPM
Esophagus	6.1 nTPM

Respiratory ciliated cells	0.0 nTPM
Bronchus	0.1 nTPM
Lung	0.0 nTPM

Smooth muscle cells	1.4 nTPM
Heart muscle	9.8 nTPM
Bronchus	2.9 nTPM
Skin	1.4 nTPM
Skeletal muscle	0.7 nTPM
Breast	0.3 nTPM
Endometrium	0.2 nTPM
Prostate	0.0 nTPM
Pancreas	0.0 nTPM
Ovary	0.0 nTPM
Adipose tissue	0.0 nTPM
Esophagus	0.0 nTPM

T-cells	25.7 nTPM
Bone marrow	48.3 nTPM
Adipose tissue	44.1 nTPM
Spleen	37.5 nTPM
Lung	34.9 nTPM
Ovary	33.3 nTPM
Skeletal muscle	33.1 nTPM
Liver	32.0 nTPM
Esophagus	26.2 nTPM
Stomach	25.0 nTPM
Lymph node	21.9 nTPM
Skin	19.8 nTPM
PBMC	18.9 nTPM
Endometrium	17.9 nTPM
Breast	17.7 nTPM
Kidney	13.7 nTPM
Colon	7.1 nTPM
Prostate	4.7 nTPM

Cholangiocytes	1.5 nTPM
Liver	1.5 nTPM

Early spermatids	4.5 nTPM
Testis	4.5 nTPM

Undifferentiated cells	0.0 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM
Rectum	0.0 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC