Single cell type - NOSIP - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	NOSIP
Gene descriptionⁱ Full gene name according to HGNC.	Nitric oxide synthase interacting protein
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	5

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	NK-cells & T-cells - Immune response (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Late spermatids, Early spermatids)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Late spermatids, Early spermatids)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

NOSIP is part of cluster 63 NK-cells & T-cells - Immune response with confidenceⁱ 1
362 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
ZNF101	Zinc finger protein 101	0.6802	63
TRAV40	T cell receptor alpha variable 40	0.6343	63
SSTR3	Somatostatin receptor 3	0.6311	63
LTB	Lymphotoxin beta	0.6247	63
LEF1	Lymphoid enhancer binding factor 1	0.6196	58
RPL38	Ribosomal protein L38	0.6193	32
TRAC	T cell receptor alpha constant	0.6123	63
FGD3	FYVE, RhoGEF and PH domain containing 3	0.6054	63
EIF3G	Eukaryotic translation initiation factor 3 subunit G	0.5898	32
TCF7	Transcription factor 7	0.5852	63
RPS27	Ribosomal protein S27	0.5824	32
TRAF3IP3	TRAF3 interacting protein 3	0.5685	63
GIMAP7	GTPase, IMAP family member 7	0.5602	63
EIF3F	Eukaryotic translation initiation factor 3 subunit F	0.5565	32
CD8B	CD8b molecule	0.5495	63

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	78.3 nTPM
Breast	95.4 nTPM
Adipose tissue	61.2 nTPM

Alveolar cells type 1	19.2 nTPM
Lung	19.2 nTPM

Alveolar cells type 2	34.9 nTPM
Lung	34.9 nTPM

Astrocytes	13.1 nTPM
Brain	13.1 nTPM

B-cells	67.3 nTPM
PBMC	115.7 nTPM
Liver	91.4 nTPM
Kidney	80.9 nTPM
Colon	70.3 nTPM
Breast	65.6 nTPM
Spleen	59.1 nTPM
Lymph node	54.4 nTPM
Stomach	45.9 nTPM
Bone marrow	45.8 nTPM
Adipose tissue	43.7 nTPM

Dendritic cells	74.7 nTPM
Breast	84.5 nTPM
PBMC	79.7 nTPM
Adipose tissue	59.8 nTPM

Endothelial cells	73.0 nTPM
Placenta	104.0 nTPM
Pancreas	100.4 nTPM
Skin	96.4 nTPM
Testis	91.8 nTPM
Prostate	87.9 nTPM
Liver	78.9 nTPM
Breast	75.5 nTPM
Heart muscle	68.5 nTPM
Skeletal muscle	68.0 nTPM
Ovary	64.2 nTPM
Esophagus	62.3 nTPM
Eye	57.4 nTPM
Endometrium	47.9 nTPM
Lung	18.7 nTPM

Fibroblasts	51.7 nTPM
Placenta	74.1 nTPM
Skin	73.5 nTPM
Ovary	62.8 nTPM
Breast	60.1 nTPM
Prostate	54.0 nTPM
Adipose tissue	46.6 nTPM
Esophagus	45.6 nTPM
Skeletal muscle	45.5 nTPM
Heart muscle	45.1 nTPM
Stomach	40.1 nTPM
Lung	21.2 nTPM

Intestinal goblet cells	108.9 nTPM
Colon	161.5 nTPM
Small intestine	94.6 nTPM
Rectum	70.6 nTPM

Macrophages	55.5 nTPM
Kidney	93.0 nTPM
Pancreas	86.8 nTPM
Stomach	67.4 nTPM
Ovary	66.4 nTPM
Prostate	63.0 nTPM
Breast	60.3 nTPM
Testis	59.8 nTPM
Bone marrow	51.2 nTPM
Spleen	46.7 nTPM
PBMC	40.9 nTPM
Esophagus	40.9 nTPM
Skeletal muscle	40.7 nTPM
Lung	39.4 nTPM
Adipose tissue	39.4 nTPM
Endometrium	35.8 nTPM

Paneth cells	138.2 nTPM
Colon	237.2 nTPM
Small intestine	101.4 nTPM
Rectum	76.1 nTPM

Plasma cells	55.4 nTPM
Spleen	73.1 nTPM
Stomach	63.6 nTPM
Adipose tissue	52.2 nTPM
Bone marrow	51.1 nTPM
Esophagus	36.9 nTPM

Respiratory ciliated cells	58.4 nTPM
Bronchus	83.3 nTPM
Lung	33.5 nTPM

Smooth muscle cells	66.1 nTPM
Pancreas	130.1 nTPM
Bronchus	114.3 nTPM
Ovary	72.2 nTPM
Skin	68.7 nTPM
Breast	59.3 nTPM
Skeletal muscle	59.0 nTPM
Heart muscle	58.2 nTPM
Prostate	50.6 nTPM
Endometrium	40.7 nTPM
Esophagus	37.2 nTPM
Adipose tissue	36.5 nTPM

T-cells	154.2 nTPM
PBMC	626.7 nTPM
Lymph node	243.0 nTPM
Skin	230.3 nTPM
Kidney	195.8 nTPM
Spleen	180.7 nTPM
Liver	140.0 nTPM
Ovary	137.9 nTPM
Bone marrow	131.2 nTPM
Adipose tissue	115.4 nTPM
Colon	109.8 nTPM
Breast	98.5 nTPM
Skeletal muscle	96.5 nTPM
Lung	95.7 nTPM
Prostate	71.1 nTPM
Stomach	66.1 nTPM
Endometrium	42.9 nTPM
Esophagus	39.3 nTPM

Basal keratinocytes	72.8 nTPM
Skin	72.8 nTPM

Basal prostatic cells	54.8 nTPM
Prostate	54.8 nTPM

Cholangiocytes	62.9 nTPM
Liver	62.9 nTPM

Collecting duct cells	51.5 nTPM
Kidney	51.5 nTPM

Undifferentiated cells	108.5 nTPM
Small intestine	129.3 nTPM
Colon	120.4 nTPM
Rectum	75.9 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC