Single cell type - ECE2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ECE2
Gene descriptionⁱ Full gene name according to HGNC.	Endothelin converting enzyme 2
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes Metabolic proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	4

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Neurons & Oligodendrocytes - Synaptic function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Late spermatids, Early spermatids, Inhibitory neurons, Extravillous trophoblasts)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Not detected in immune cells
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Not detected
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enriched (REH)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in some
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Late spermatids, Early spermatids, Inhibitory neurons, Extravillous trophoblasts)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

ECE2 is part of cluster 8 Neurons & Oligodendrocytes - Synaptic function with confidenceⁱ 1
719 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
DLX1	Distal-less homeobox 1	0.5703	8
APBA1	Amyloid beta precursor protein binding family A member 1	0.5644	8
SLC10A4	Solute carrier family 10 member 4	0.5498	8
GRIK2	Glutamate ionotropic receptor kainate type subunit 2	0.5269	8
AFAP1	Actin filament associated protein 1	0.5173	8
ADARB2	Adenosine deaminase RNA specific B2 (inactive)	0.5169	8
ADRA1A	Adrenoceptor alpha 1A	0.5165	8
HTR5A	5-hydroxytryptamine receptor 5A	0.5157	8
DAB1	DAB adaptor protein 1	0.5132	8
GALNTL6	Polypeptide N-acetylgalactosaminyltransferase like 6	0.5115	8
C8orf34	Chromosome 8 open reading frame 34	0.5050	8
INPP4B	Inositol polyphosphate-4-phosphatase type II B	0.5044	8
GRID2IP	Grid2 interacting protein	0.5039	8
SNAPC3	Small nuclear RNA activating complex polypeptide 3	0.5017	31
ZBTB34	Zinc finger and BTB domain containing 34	0.5011	31

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	0.0 nTPM
Adipose tissue	0.0 nTPM
Breast	0.0 nTPM

Alveolar cells type 1	0.0 nTPM
Lung	0.0 nTPM

Alveolar cells type 2	0.0 nTPM
Lung	0.0 nTPM

Astrocytes	5.2 nTPM
Brain	5.2 nTPM

B-cells	3.8 nTPM
Liver	18.5 nTPM
Lymph node	5.2 nTPM
Colon	4.8 nTPM
PBMC	2.8 nTPM
Spleen	2.8 nTPM
Bone marrow	2.5 nTPM
Stomach	1.4 nTPM
Kidney	0.0 nTPM
Adipose tissue	0.0 nTPM
Breast	0.0 nTPM

Dendritic cells	2.7 nTPM
PBMC	8.0 nTPM
Adipose tissue	0.0 nTPM
Breast	0.0 nTPM

Endothelial cells	3.3 nTPM
Skin	9.9 nTPM
Prostate	8.9 nTPM
Placenta	8.6 nTPM
Esophagus	7.3 nTPM
Liver	3.7 nTPM
Testis	3.0 nTPM
Skeletal muscle	2.3 nTPM
Heart muscle	1.4 nTPM
Eye	0.8 nTPM
Lung	0.0 nTPM
Pancreas	0.0 nTPM
Endometrium	0.0 nTPM
Ovary	0.0 nTPM
Breast	0.0 nTPM

Fibroblasts	3.5 nTPM
Prostate	12.0 nTPM
Placenta	10.3 nTPM
Skin	6.2 nTPM
Stomach	6.0 nTPM
Heart muscle	1.5 nTPM
Skeletal muscle	1.3 nTPM
Esophagus	1.2 nTPM
Lung	0.0 nTPM
Ovary	0.0 nTPM
Adipose tissue	0.0 nTPM
Breast	0.0 nTPM

Intestinal goblet cells	12.8 nTPM
Colon	14.3 nTPM
Small intestine	13.0 nTPM
Rectum	11.3 nTPM

Macrophages	4.1 nTPM
PBMC	13.6 nTPM
Prostate	9.1 nTPM
Stomach	8.8 nTPM
Testis	6.9 nTPM
Esophagus	6.3 nTPM
Spleen	6.2 nTPM
Skeletal muscle	5.3 nTPM
Bone marrow	5.0 nTPM
Kidney	0.0 nTPM
Lung	0.0 nTPM
Pancreas	0.0 nTPM
Endometrium	0.0 nTPM
Ovary	0.0 nTPM
Adipose tissue	0.0 nTPM
Breast	0.0 nTPM

Paneth cells	5.9 nTPM
Rectum	10.7 nTPM
Small intestine	3.7 nTPM
Colon	3.3 nTPM

Plasma cells	12.5 nTPM
Spleen	22.1 nTPM
Bone marrow	19.8 nTPM
Esophagus	12.3 nTPM
Stomach	8.2 nTPM
Adipose tissue	0.0 nTPM

Respiratory ciliated cells	2.2 nTPM
Bronchus	4.4 nTPM
Lung	0.0 nTPM

Smooth muscle cells	5.1 nTPM
Bronchus	38.0 nTPM
Prostate	8.4 nTPM
Skin	5.2 nTPM
Esophagus	3.1 nTPM
Skeletal muscle	1.0 nTPM
Heart muscle	0.8 nTPM
Pancreas	0.0 nTPM
Endometrium	0.0 nTPM
Ovary	0.0 nTPM
Adipose tissue	0.0 nTPM
Breast	0.0 nTPM

T-cells	2.3 nTPM
Esophagus	13.1 nTPM
Lymph node	5.3 nTPM
Skin	5.0 nTPM
Liver	4.1 nTPM
Bone marrow	3.1 nTPM
PBMC	2.5 nTPM
Colon	1.9 nTPM
Spleen	1.9 nTPM
Skeletal muscle	1.5 nTPM
Stomach	1.2 nTPM
Kidney	0.0 nTPM
Lung	0.0 nTPM
Prostate	0.0 nTPM
Endometrium	0.0 nTPM
Ovary	0.0 nTPM
Adipose tissue	0.0 nTPM
Breast	0.0 nTPM

Basal keratinocytes	38.2 nTPM
Skin	38.2 nTPM

Basal prostatic cells	18.2 nTPM
Prostate	18.2 nTPM

Basal respiratory cells	15.5 nTPM
Bronchus	15.5 nTPM

Basal squamous epithelial cells	20.5 nTPM
Esophagus	20.5 nTPM

Undifferentiated cells	13.8 nTPM
Small intestine	13.9 nTPM
Rectum	13.9 nTPM
Colon	13.6 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC