Single cell type - RNF217 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	RNF217
Gene descriptionⁱ Full gene name according to HGNC.	Ring finger protein 217
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes Metabolic proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	5

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Spermatids - Flagellum & Golgi organization (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Early spermatids, Oligodendrocytes)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Not detected in immune cells
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Not detected
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Early spermatids, Oligodendrocytes)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

RNF217 is part of cluster 23 Spermatids - Flagellum & Golgi organization with confidenceⁱ 1
311 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
GOLGA6B	Golgin A6 family member B	0.5293	23
STK3	Serine/threonine kinase 3	0.5164	64
TFDP2	Transcription factor Dp-2	0.5154	23
CDKL3	Cyclin dependent kinase like 3	0.5013	23
NOL10	Nucleolar protein 10	0.4979	23
DHX33	DEAH-box helicase 33	0.4652	29
METTL6	Methyltransferase like 6	0.4585	23
OTUD4	OTU deubiquitinase 4	0.4562	44
SNCAIP	Synuclein alpha interacting protein	0.4558	64
HEATR4	HEAT repeat containing 4	0.4526	23
RABEPK	Rab9 effector protein with kelch motifs	0.4523	23
NUDCD1	NudC domain containing 1	0.4511	23
WRN	WRN RecQ like helicase	0.4497	29
SSUH2	Ssu-2 homolog	0.4482	29
TMC7	Transmembrane channel like 7	0.4462	23

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	25.0 nTPM
Adipose tissue	32.3 nTPM
Breast	17.6 nTPM

Alveolar cells type 1	22.4 nTPM
Lung	22.4 nTPM

Alveolar cells type 2	21.0 nTPM
Lung	21.0 nTPM

Astrocytes	49.0 nTPM
Brain	49.0 nTPM

B-cells	0.6 nTPM
Breast	4.0 nTPM
Liver	1.8 nTPM
Spleen	0.3 nTPM
Lymph node	0.2 nTPM
Colon	0.0 nTPM
Kidney	0.0 nTPM
PBMC	0.0 nTPM
Adipose tissue	0.0 nTPM
Bone marrow	0.0 nTPM
Stomach	0.0 nTPM

Dendritic cells	0.9 nTPM
Adipose tissue	2.5 nTPM
Breast	0.4 nTPM
PBMC	0.0 nTPM

Endothelial cells	19.0 nTPM
Heart muscle	27.5 nTPM
Skeletal muscle	26.2 nTPM
Esophagus	25.9 nTPM
Endometrium	22.2 nTPM
Liver	22.0 nTPM
Prostate	21.1 nTPM
Skin	20.9 nTPM
Testis	16.3 nTPM
Breast	16.3 nTPM
Eye	16.2 nTPM
Lung	15.6 nTPM
Pancreas	15.5 nTPM
Ovary	13.7 nTPM
Placenta	6.8 nTPM

Fibroblasts	23.1 nTPM
Skin	46.5 nTPM
Adipose tissue	33.4 nTPM
Lung	29.0 nTPM
Skeletal muscle	25.6 nTPM
Prostate	24.9 nTPM
Esophagus	23.2 nTPM
Heart muscle	22.3 nTPM
Breast	19.2 nTPM
Stomach	13.4 nTPM
Placenta	13.0 nTPM
Ovary	3.5 nTPM

Intestinal goblet cells	2.4 nTPM
Rectum	3.7 nTPM
Small intestine	2.8 nTPM
Colon	0.7 nTPM

Macrophages	11.3 nTPM
Adipose tissue	20.8 nTPM
Esophagus	18.9 nTPM
Skeletal muscle	18.0 nTPM
Ovary	15.5 nTPM
Testis	15.1 nTPM
Prostate	13.8 nTPM
Spleen	13.5 nTPM
Lung	13.0 nTPM
Breast	10.7 nTPM
Bone marrow	10.0 nTPM
Endometrium	9.6 nTPM
Kidney	8.3 nTPM
Stomach	2.9 nTPM
PBMC	0.0 nTPM
Pancreas	0.0 nTPM

Paneth cells	2.0 nTPM
Rectum	6.1 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM

Plasma cells	0.4 nTPM
Adipose tissue	1.9 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM
Stomach	0.0 nTPM

Respiratory ciliated cells	9.6 nTPM
Bronchus	13.4 nTPM
Lung	5.8 nTPM

Smooth muscle cells	12.3 nTPM
Ovary	24.8 nTPM
Skin	18.9 nTPM
Prostate	17.5 nTPM
Adipose tissue	16.4 nTPM
Endometrium	14.6 nTPM
Skeletal muscle	9.4 nTPM
Pancreas	9.2 nTPM
Breast	7.9 nTPM
Esophagus	6.2 nTPM
Bronchus	5.6 nTPM
Heart muscle	4.7 nTPM

T-cells	1.8 nTPM
Endometrium	8.0 nTPM
Esophagus	6.6 nTPM
Skin	5.0 nTPM
Lung	4.5 nTPM
Skeletal muscle	2.3 nTPM
Liver	1.5 nTPM
Breast	0.6 nTPM
Colon	0.5 nTPM
Bone marrow	0.4 nTPM
Spleen	0.4 nTPM
Adipose tissue	0.3 nTPM
Lymph node	0.3 nTPM
Stomach	0.2 nTPM
PBMC	0.1 nTPM
Kidney	0.0 nTPM
Prostate	0.0 nTPM
Ovary	0.0 nTPM

Basal keratinocytes	48.2 nTPM
Skin	48.2 nTPM

Basal prostatic cells	15.8 nTPM
Prostate	15.8 nTPM

Basal squamous epithelial cells	18.2 nTPM
Esophagus	18.2 nTPM

Cholangiocytes	4.3 nTPM
Liver	4.3 nTPM

Undifferentiated cells	4.3 nTPM
Small intestine	8.0 nTPM
Rectum	2.7 nTPM
Colon	2.2 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC