Single cell type - NONO - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	NONO
Gene descriptionⁱ Full gene name according to HGNC.	Non-POU domain containing octamer binding
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Cancer-related genes Disease related genes Human disease related genes Plasma proteins Transcription factors
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	18

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Non-specific - Unknown function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low cell type specificity
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Low cell type specificity

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

NONO is part of cluster 10 Non-specific - Unknown function with confidenceⁱ 0.99
482 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
HMGB1	High mobility group box 1	0.6376	12
KHDRBS1	KH RNA binding domain containing, signal transduction associated 1	0.5582	59
HNRNPR	Heterogeneous nuclear ribonucleoprotein R	0.5564	10
RBBP4	RB binding protein 4, chromatin remodeling factor	0.5533	59
RBMX	RNA binding motif protein X-linked	0.5457	32
HMGN2	High mobility group nucleosomal binding domain 2	0.5443	12
SET	SET nuclear proto-oncogene	0.5401	39
MED1	Mediator complex subunit 1	0.5295	10
PPIA	Peptidylprolyl isomerase A	0.5190	32
PPIH	Peptidylprolyl isomerase H	0.5183	10
ERCC6L	ERCC excision repair 6 like, spindle assembly checkpoint helicase	0.5137	12
HNRNPD	Heterogeneous nuclear ribonucleoprotein D	0.5125	10
HNRNPA3	Heterogeneous nuclear ribonucleoprotein A3	0.5072	10
STMN1	Stathmin 1	0.4970	12
DDX42	DEAD-box helicase 42	0.4954	10

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	72.9 nTPM
Adipose tissue	86.4 nTPM
Breast	59.5 nTPM

Alveolar cells type 1	76.9 nTPM
Lung	76.9 nTPM

Alveolar cells type 2	81.1 nTPM
Lung	81.1 nTPM

Astrocytes	16.3 nTPM
Brain	16.3 nTPM

B-cells	97.2 nTPM
Liver	121.8 nTPM
Spleen	120.1 nTPM
Adipose tissue	119.8 nTPM
Bone marrow	103.0 nTPM
Lymph node	100.7 nTPM
Stomach	100.3 nTPM
Colon	95.8 nTPM
PBMC	77.1 nTPM
Kidney	71.4 nTPM
Breast	61.5 nTPM

Basal squamous epithelial cells	116.7 nTPM
Esophagus	116.7 nTPM

Dendritic cells	103.6 nTPM
Adipose tissue	140.4 nTPM
PBMC	95.6 nTPM
Breast	74.7 nTPM

Endothelial cells	95.4 nTPM
Prostate	137.1 nTPM
Endometrium	125.5 nTPM
Skin	116.9 nTPM
Testis	116.5 nTPM
Ovary	111.8 nTPM
Placenta	100.0 nTPM
Esophagus	97.3 nTPM
Pancreas	96.5 nTPM
Liver	85.3 nTPM
Breast	80.3 nTPM
Heart muscle	68.9 nTPM
Lung	68.6 nTPM
Eye	68.0 nTPM
Skeletal muscle	62.7 nTPM

Enteroendocrine cells	139.8 nTPM
Colon	177.7 nTPM
Rectum	102.0 nTPM

Erythroid cells	144.6 nTPM
Bone marrow	241.3 nTPM
Liver	48.0 nTPM

Fibroblasts	93.9 nTPM
Ovary	171.9 nTPM
Lung	155.3 nTPM
Skin	102.8 nTPM
Placenta	102.4 nTPM
Prostate	101.3 nTPM
Esophagus	86.1 nTPM
Adipose tissue	76.0 nTPM
Stomach	69.8 nTPM
Breast	61.7 nTPM
Skeletal muscle	54.9 nTPM
Heart muscle	51.3 nTPM

Intestinal goblet cells	105.6 nTPM
Rectum	111.6 nTPM
Small intestine	107.4 nTPM
Colon	97.9 nTPM

Macrophages	95.0 nTPM
Adipose tissue	143.3 nTPM
Spleen	134.8 nTPM
Bone marrow	130.0 nTPM
Endometrium	114.5 nTPM
Esophagus	110.1 nTPM
Pancreas	94.4 nTPM
Kidney	93.8 nTPM
Prostate	90.5 nTPM
Testis	90.1 nTPM
Ovary	86.1 nTPM
Skeletal muscle	84.9 nTPM
Stomach	82.1 nTPM
Lung	79.9 nTPM
Breast	63.6 nTPM
PBMC	27.3 nTPM

Paneth cells	75.4 nTPM
Rectum	96.5 nTPM
Small intestine	90.1 nTPM
Colon	39.6 nTPM

Plasma cells	115.2 nTPM
Spleen	164.1 nTPM
Bone marrow	156.5 nTPM
Adipose tissue	94.8 nTPM
Stomach	92.6 nTPM
Esophagus	67.8 nTPM

Collecting duct cells	44.1 nTPM
Kidney	44.1 nTPM

Cone photoreceptor cells	41.7 nTPM
Eye	41.7 nTPM

Cytotrophoblasts	132.3 nTPM
Placenta	132.3 nTPM

Distal enterocytes	52.0 nTPM
Rectum	55.8 nTPM
Colon	48.2 nTPM

Respiratory ciliated cells	71.1 nTPM
Bronchus	83.9 nTPM
Lung	58.3 nTPM

Smooth muscle cells	127.8 nTPM
Bronchus	364.7 nTPM
Pancreas	137.0 nTPM
Endometrium	136.7 nTPM
Ovary	135.2 nTPM
Prostate	122.3 nTPM
Skin	109.8 nTPM
Esophagus	102.3 nTPM
Adipose tissue	100.3 nTPM
Heart muscle	66.6 nTPM
Skeletal muscle	65.4 nTPM
Breast	65.2 nTPM

T-cells	108.5 nTPM
Esophagus	170.3 nTPM
Adipose tissue	142.1 nTPM
Endometrium	132.7 nTPM
PBMC	125.1 nTPM
Spleen	118.7 nTPM
Stomach	118.2 nTPM
Liver	114.2 nTPM
Bone marrow	114.2 nTPM
Lymph node	109.5 nTPM
Colon	106.4 nTPM
Skin	106.4 nTPM
Skeletal muscle	95.2 nTPM
Kidney	89.0 nTPM
Lung	85.0 nTPM
Breast	77.4 nTPM
Prostate	75.7 nTPM
Ovary	64.5 nTPM

Undifferentiated cells	117.2 nTPM
Small intestine	143.4 nTPM
Rectum	109.7 nTPM
Colon	98.4 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC