Single cell type - DIAPH2 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	DIAPH2
Gene descriptionⁱ Full gene name according to HGNC.	Diaphanous related formin 2
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Human disease related genes Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	5

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Neurons & Oligodendrocytes - Neuronal signaling (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Microglial cells, Oligodendrocyte precursor cells, Late spermatids, Excitatory neurons)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Microglial cells, Oligodendrocyte precursor cells, Late spermatids, Excitatory neurons)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

DIAPH2 is part of cluster 21 Neurons & Oligodendrocytes - Neuronal signaling with confidenceⁱ 1
589 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
PDZD2	PDZ domain containing 2	0.5733	21
NTRK3	Neurotrophic receptor tyrosine kinase 3	0.5617	21
TMC2	Transmembrane channel like 2	0.5562	68
CDKL1	Cyclin dependent kinase like 1	0.5433	21
FAM13A	Family with sequence similarity 13 member A	0.5348	21
CA10	Carbonic anhydrase 10	0.5300	21
USP25	Ubiquitin specific peptidase 25	0.5188	21
USP43	Ubiquitin specific peptidase 43	0.5143	44
SYN3	Synapsin III	0.5087	21
OTOGL	Otogelin like	0.5002	21
GSG1L	GSG1 like	0.4955	21
ITPKA	Inositol-trisphosphate 3-kinase A	0.4887	31
PTPRG	Protein tyrosine phosphatase receptor type G	0.4840	68
ATP7B	ATPase copper transporting beta	0.4835	9
MED12L	Mediator complex subunit 12L	0.4820	21

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	61.7 nTPM
Adipose tissue	62.3 nTPM
Breast	61.0 nTPM

Alveolar cells type 1	32.1 nTPM
Lung	32.1 nTPM

Alveolar cells type 2	51.1 nTPM
Lung	51.1 nTPM

Astrocytes	21.2 nTPM
Brain	21.2 nTPM

B-cells	18.8 nTPM
Adipose tissue	34.1 nTPM
Stomach	28.2 nTPM
PBMC	27.1 nTPM
Bone marrow	22.1 nTPM
Spleen	21.1 nTPM
Lymph node	15.2 nTPM
Breast	14.7 nTPM
Colon	14.4 nTPM
Liver	5.9 nTPM
Kidney	4.8 nTPM

Dendritic cells	21.5 nTPM
PBMC	27.9 nTPM
Adipose tissue	23.1 nTPM
Breast	13.5 nTPM

Endothelial cells	31.2 nTPM
Skeletal muscle	51.9 nTPM
Ovary	49.0 nTPM
Breast	46.2 nTPM
Prostate	45.6 nTPM
Pancreas	42.5 nTPM
Esophagus	36.2 nTPM
Heart muscle	32.8 nTPM
Skin	31.3 nTPM
Lung	28.1 nTPM
Endometrium	22.2 nTPM
Eye	18.6 nTPM
Testis	13.3 nTPM
Placenta	13.1 nTPM
Liver	5.5 nTPM

Fibroblasts	35.2 nTPM
Adipose tissue	63.4 nTPM
Prostate	48.3 nTPM
Skeletal muscle	47.0 nTPM
Stomach	43.0 nTPM
Breast	36.6 nTPM
Esophagus	35.1 nTPM
Lung	33.5 nTPM
Heart muscle	31.2 nTPM
Skin	27.3 nTPM
Ovary	13.4 nTPM
Placenta	8.8 nTPM

Intestinal goblet cells	32.3 nTPM
Colon	42.9 nTPM
Rectum	33.1 nTPM
Small intestine	20.8 nTPM

Macrophages	34.7 nTPM
Skeletal muscle	55.7 nTPM
Spleen	53.9 nTPM
Adipose tissue	53.8 nTPM
Bone marrow	43.7 nTPM
Endometrium	41.7 nTPM
Stomach	41.0 nTPM
Testis	39.5 nTPM
Lung	37.6 nTPM
Ovary	35.6 nTPM
Breast	28.4 nTPM
Kidney	28.0 nTPM
Esophagus	20.4 nTPM
Prostate	13.8 nTPM
PBMC	13.6 nTPM
Pancreas	12.8 nTPM

Paneth cells	39.3 nTPM
Rectum	40.6 nTPM
Colon	39.6 nTPM
Small intestine	37.6 nTPM

Plasma cells	11.6 nTPM
Adipose tissue	29.7 nTPM
Esophagus	12.3 nTPM
Stomach	5.8 nTPM
Spleen	5.1 nTPM
Bone marrow	5.0 nTPM

Respiratory ciliated cells	57.1 nTPM
Bronchus	67.6 nTPM
Lung	46.6 nTPM

Smooth muscle cells	32.7 nTPM
Adipose tissue	78.1 nTPM
Skeletal muscle	63.1 nTPM
Heart muscle	39.3 nTPM
Prostate	36.0 nTPM
Breast	33.3 nTPM
Esophagus	26.4 nTPM
Ovary	19.0 nTPM
Bronchus	18.9 nTPM
Endometrium	18.1 nTPM
Skin	15.0 nTPM
Pancreas	13.1 nTPM

T-cells	19.0 nTPM
Adipose tissue	42.2 nTPM
Skeletal muscle	32.1 nTPM
Endometrium	25.1 nTPM
Lung	22.8 nTPM
Esophagus	19.7 nTPM
Stomach	18.6 nTPM
Breast	18.3 nTPM
Bone marrow	16.9 nTPM
PBMC	16.5 nTPM
Ovary	15.6 nTPM
Lymph node	15.4 nTPM
Spleen	14.6 nTPM
Skin	14.3 nTPM
Prostate	14.2 nTPM
Kidney	13.1 nTPM
Liver	12.4 nTPM
Colon	10.5 nTPM

Basal keratinocytes	10.8 nTPM
Skin	10.8 nTPM

Basal prostatic cells	6.8 nTPM
Prostate	6.8 nTPM

Basal respiratory cells	11.9 nTPM
Bronchus	11.9 nTPM

Basal squamous epithelial cells	11.4 nTPM
Esophagus	11.4 nTPM

Undifferentiated cells	32.5 nTPM
Colon	43.5 nTPM
Rectum	34.1 nTPM
Small intestine	19.9 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC