Single cell type - CAPN5 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	CAPN5
Gene descriptionⁱ Full gene name according to HGNC.	Calpain 5
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Enzymes Human disease related genes Potential drug targets
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	5

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Intestinal epithelial cells - Unknown function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Distal enterocytes, Intestinal goblet cells, Gastric mucus-secreting cells, Proximal enterocytes, Paneth cells, Undifferentiated cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enhanced (gdT-cell)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in single
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (OE19)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Distal enterocytes, Intestinal goblet cells, Gastric mucus-secreting cells, Proximal enterocytes, Paneth cells, Undifferentiated cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

CAPN5 is part of cluster 53 Intestinal epithelial cells - Unknown function with confidenceⁱ 1
611 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
HEPH	Hephaestin	0.7873	53
ST6GALNAC6	ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 6	0.7817	53
GALNT12	Polypeptide N-acetylgalactosaminyltransferase 12	0.7800	27
PARM1	Prostate androgen-regulated mucin-like protein 1	0.7744	53
ENTPD5	Ectonucleoside triphosphate diphosphohydrolase 5 (inactive)	0.7634	53
HOXA13	Homeobox A13	0.7573	53
FAM3D	FAM3 metabolism regulating signaling molecule D	0.7462	53
FOXH1	Forkhead box H1	0.7436	53
LGALS9C	Galectin 9C	0.7388	27
ITM2C	Integral membrane protein 2C	0.7382	53
CDX2	Caudal type homeobox 2	0.7368	53
FZD5	Frizzled class receptor 5	0.7323	53
PIGZ	Phosphatidylinositol glycan anchor biosynthesis class Z	0.7262	53
B3GALT5	Beta-1,3-galactosyltransferase 5	0.7182	53
PLA2G10	Phospholipase A2 group X	0.7160	53

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	1.6 nTPM
Breast	1.8 nTPM
Adipose tissue	1.4 nTPM

Alveolar cells type 1	0.0 nTPM
Lung	0.0 nTPM

Alveolar cells type 2	0.5 nTPM
Lung	0.5 nTPM

Astrocytes	4.2 nTPM
Brain	4.2 nTPM

B-cells	1.0 nTPM
Kidney	4.8 nTPM
Stomach	1.8 nTPM
Colon	1.6 nTPM
Liver	1.3 nTPM
Bone marrow	0.8 nTPM
PBMC	0.0 nTPM
Adipose tissue	0.0 nTPM
Lymph node	0.0 nTPM
Spleen	0.0 nTPM
Breast	0.0 nTPM

Dendritic cells	3.4 nTPM
PBMC	4.0 nTPM
Breast	3.3 nTPM
Adipose tissue	2.8 nTPM

Endothelial cells	2.4 nTPM
Testis	6.5 nTPM
Ovary	5.2 nTPM
Pancreas	3.9 nTPM
Endometrium	3.1 nTPM
Esophagus	2.6 nTPM
Heart muscle	2.5 nTPM
Skin	2.3 nTPM
Breast	2.1 nTPM
Liver	1.8 nTPM
Skeletal muscle	1.0 nTPM
Eye	0.8 nTPM
Prostate	0.7 nTPM
Placenta	0.6 nTPM
Lung	0.0 nTPM

Fibroblasts	7.6 nTPM
Esophagus	13.3 nTPM
Stomach	11.9 nTPM
Adipose tissue	10.6 nTPM
Breast	9.2 nTPM
Skeletal muscle	7.9 nTPM
Skin	7.8 nTPM
Ovary	7.0 nTPM
Placenta	5.9 nTPM
Heart muscle	4.6 nTPM
Lung	3.4 nTPM
Prostate	1.9 nTPM

Intestinal goblet cells	89.8 nTPM
Rectum	95.0 nTPM
Small intestine	90.8 nTPM
Colon	83.6 nTPM

Macrophages	1.2 nTPM
Stomach	5.9 nTPM
Prostate	2.2 nTPM
Endometrium	2.0 nTPM
Esophagus	1.6 nTPM
Lung	1.5 nTPM
Ovary	1.3 nTPM
Breast	1.1 nTPM
Skeletal muscle	1.0 nTPM
Adipose tissue	0.8 nTPM
Kidney	0.0 nTPM
PBMC	0.0 nTPM
Testis	0.0 nTPM
Pancreas	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM

Paneth cells	72.6 nTPM
Rectum	101.3 nTPM
Colon	79.0 nTPM
Small intestine	37.6 nTPM

Plasma cells	0.9 nTPM
Bone marrow	1.7 nTPM
Stomach	1.4 nTPM
Adipose tissue	1.3 nTPM
Spleen	0.3 nTPM
Esophagus	0.0 nTPM

Respiratory ciliated cells	11.1 nTPM
Bronchus	17.9 nTPM
Lung	4.4 nTPM

Smooth muscle cells	4.1 nTPM
Skin	6.9 nTPM
Esophagus	6.2 nTPM
Bronchus	5.6 nTPM
Pancreas	5.5 nTPM
Endometrium	4.6 nTPM
Ovary	4.0 nTPM
Adipose tissue	3.3 nTPM
Skeletal muscle	3.2 nTPM
Prostate	2.5 nTPM
Breast	2.1 nTPM
Heart muscle	0.6 nTPM

T-cells	1.5 nTPM
Kidney	4.2 nTPM
Colon	3.3 nTPM
Skin	3.3 nTPM
Breast	2.3 nTPM
Ovary	2.2 nTPM
Stomach	1.9 nTPM
Endometrium	1.5 nTPM
Bone marrow	1.5 nTPM
Spleen	1.4 nTPM
Skeletal muscle	1.3 nTPM
Liver	1.2 nTPM
Adipose tissue	1.1 nTPM
PBMC	0.6 nTPM
Lung	0.0 nTPM
Prostate	0.0 nTPM
Esophagus	0.0 nTPM
Lymph node	0.0 nTPM

Cholangiocytes	24.3 nTPM
Liver	24.3 nTPM

Collecting duct cells	23.9 nTPM
Kidney	23.9 nTPM

Cytotrophoblasts	0.1 nTPM
Placenta	0.1 nTPM

Distal enterocytes	170.3 nTPM
Rectum	188.9 nTPM
Colon	151.7 nTPM

Undifferentiated cells	69.2 nTPM
Colon	90.3 nTPM
Rectum	73.1 nTPM
Small intestine	44.2 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC