Single cell type - CD8A - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	CD8A
Gene descriptionⁱ Full gene name according to HGNC.	CD8a molecule
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	CD markers Disease related genes Human disease related genes
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	4

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	T-cells - T-cell receptor (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Group enriched (T-cells, dendritic cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Group enriched (memory CD8 T-cell, naive CD8 T-cell, MAIT T-cell)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (JURKAT, MOLT-4, RPMI-8226)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in some
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Group enriched (T-cells, dendritic cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

CD8A is part of cluster 58 T-cells - T-cell receptor with confidenceⁱ 1
180 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
TRGV10	T cell receptor gamma variable 10 (non-functional)	0.8985	58
TRGV3	T cell receptor gamma variable 3	0.8908	58
ZAP70	Zeta chain of T cell receptor associated protein kinase 70	0.8873	63
CD3E	CD3e molecule	0.8788	58
TRBV4-2	T cell receptor beta variable 4-2	0.8619	58
TRGV8	T cell receptor gamma variable 8	0.8578	58
TRBV13	T cell receptor beta variable 13	0.8559	58
TRAV22	T cell receptor alpha variable 22	0.8537	58
TRGV1	T cell receptor gamma variable 1 (non-functional)	0.8525	58
TRGV11	T cell receptor gamma variable 11 (non-functional)	0.8506	58
TRDV1	T cell receptor delta variable 1	0.8454	58
TRGV2	T cell receptor gamma variable 2	0.8440	58
TRAV29DV5	T cell receptor alpha variable 29/delta variable 5	0.8415	58
TRBV7-2	T cell receptor beta variable 7-2	0.8410	58
ZNF80	Zinc finger protein 80	0.8403	24

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	2.2 nTPM
Breast	3.2 nTPM
Adipose tissue	1.1 nTPM

Alveolar cells type 1	0.0 nTPM
Lung	0.0 nTPM

Alveolar cells type 2	4.6 nTPM
Lung	4.6 nTPM

Astrocytes	0.0 nTPM
Brain	0.0 nTPM

B-cells	5.7 nTPM
PBMC	21.4 nTPM
Adipose tissue	8.1 nTPM
Breast	8.1 nTPM
Stomach	5.9 nTPM
Colon	3.2 nTPM
Bone marrow	3.2 nTPM
Liver	2.8 nTPM
Lymph node	2.3 nTPM
Spleen	2.1 nTPM
Kidney	0.0 nTPM

Dendritic cells	111.7 nTPM
Breast	258.7 nTPM
Adipose tissue	72.5 nTPM
PBMC	4.0 nTPM

Endothelial cells	1.3 nTPM
Skin	3.4 nTPM
Lung	3.1 nTPM
Heart muscle	2.8 nTPM
Esophagus	2.6 nTPM
Breast	2.6 nTPM
Liver	1.8 nTPM
Skeletal muscle	0.6 nTPM
Endometrium	0.5 nTPM
Eye	0.0 nTPM
Placenta	0.0 nTPM
Prostate	0.0 nTPM
Testis	0.0 nTPM
Pancreas	0.0 nTPM
Ovary	0.0 nTPM

Fibroblasts	2.6 nTPM
Stomach	17.8 nTPM
Breast	3.0 nTPM
Lung	2.2 nTPM
Skin	2.1 nTPM
Esophagus	1.4 nTPM
Placenta	0.8 nTPM
Heart muscle	0.5 nTPM
Skeletal muscle	0.3 nTPM
Adipose tissue	0.1 nTPM
Prostate	0.0 nTPM
Ovary	0.0 nTPM

Intestinal goblet cells	0.3 nTPM
Colon	0.7 nTPM
Small intestine	0.0 nTPM
Rectum	0.0 nTPM

Macrophages	6.6 nTPM
Breast	27.5 nTPM
PBMC	27.3 nTPM
Bone marrow	13.8 nTPM
Adipose tissue	11.9 nTPM
Spleen	8.3 nTPM
Stomach	5.9 nTPM
Lung	1.2 nTPM
Prostate	1.2 nTPM
Skeletal muscle	0.9 nTPM
Kidney	0.8 nTPM
Endometrium	0.4 nTPM
Ovary	0.4 nTPM
Testis	0.0 nTPM
Pancreas	0.0 nTPM
Esophagus	0.0 nTPM

Paneth cells	0.0 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM
Rectum	0.0 nTPM

Plasma cells	30.8 nTPM
Adipose tissue	99.9 nTPM
Bone marrow	33.0 nTPM
Spleen	10.7 nTPM
Stomach	10.2 nTPM
Esophagus	0.0 nTPM

Respiratory ciliated cells	1.5 nTPM
Lung	2.9 nTPM
Bronchus	0.0 nTPM

Smooth muscle cells	1.4 nTPM
Breast	8.3 nTPM
Skin	3.1 nTPM
Skeletal muscle	1.2 nTPM
Heart muscle	1.0 nTPM
Prostate	0.9 nTPM
Adipose tissue	0.6 nTPM
Endometrium	0.4 nTPM
Ovary	0.2 nTPM
Pancreas	0.0 nTPM
Bronchus	0.0 nTPM
Esophagus	0.0 nTPM

T-cells	242.4 nTPM
Stomach	595.3 nTPM
Bone marrow	396.5 nTPM
Ovary	371.5 nTPM
Spleen	370.4 nTPM
Colon	338.3 nTPM
Esophagus	294.8 nTPM
Adipose tissue	291.2 nTPM
Breast	264.2 nTPM
PBMC	190.1 nTPM
Skeletal muscle	184.1 nTPM
Lymph node	169.0 nTPM
Skin	154.3 nTPM
Liver	151.5 nTPM
Kidney	120.5 nTPM
Endometrium	97.6 nTPM
Prostate	71.1 nTPM
Lung	60.7 nTPM

Basal keratinocytes	2.0 nTPM
Skin	2.0 nTPM

Excitatory neurons	4.2 nTPM
Brain	4.2 nTPM

Undifferentiated cells	0.6 nTPM
Colon	1.7 nTPM
Small intestine	0.0 nTPM
Rectum	0.0 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC