Single cell type - ADAMTS4 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ADAMTS4
Gene descriptionⁱ Full gene name according to HGNC.	ADAM metallopeptidase with thrombospondin type 1 motif 4
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Cancer-related genes Enzymes Transporters
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane,Secreted
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	2

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Smooth muscle cells - ECM organization (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Smooth muscle cells, Endothelial cells, Fibroblasts, Adipocytes, Oligodendrocytes, Sertoli cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enriched (plasmacytoid DC)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in single
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (hTEC/SVTERT24-B, HUVEC TERT2, SH-SY5Y, TIME, U-138 MG, U-251 MG)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in some
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level
Extracellular locationⁱ A possible secretion of the protein was predicted based on N-terminal signal sequence (signal peptide) predictions and transmembrane region predictions, and subsequently annotated for its local or systemic secretion based on literature, function, and protein and RNA expression data. Read more	Protein predicted to be secreted

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Smooth muscle cells, Endothelial cells, Fibroblasts, Adipocytes, Oligodendrocytes, Sertoli cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

ADAMTS4 is part of cluster 48 Smooth muscle cells - ECM organization with confidenceⁱ 1
270 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
IL6	Interleukin 6	0.8800	48
GJA4	Gap junction protein alpha 4	0.8316	48
C1QTNF1	C1q and TNF related 1	0.8280	48
CRISPLD2	Cysteine rich secretory protein LCCL domain containing 2	0.8217	48
CCL8	C-C motif chemokine ligand 8	0.8102	48
RGS5	Regulator of G protein signaling 5	0.7947	48
GPR4	G protein-coupled receptor 4	0.7840	48
KCNE4	Potassium voltage-gated channel subfamily E regulatory subunit 4	0.7720	48
ADAMTS1	ADAM metallopeptidase with thrombospondin type 1 motif 1	0.7678	48
MT1A	Metallothionein 1A	0.7677	48
CCL26	C-C motif chemokine ligand 26	0.7614	48
COX4I2	Cytochrome c oxidase subunit 4I2	0.7508	48
PTGDR2	Prostaglandin D2 receptor 2	0.7506	48
C11orf96	Chromosome 11 open reading frame 96	0.7476	48
NMS	Neuromedin S	0.7405	48

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Alveolar cells type 1	0.0 nTPM
Lung	0.0 nTPM

Alveolar cells type 2	4.1 nTPM
Lung	4.1 nTPM

Astrocytes	0.0 nTPM
Brain	0.0 nTPM

B-cells	0.2 nTPM
Breast	1.3 nTPM
Spleen	0.7 nTPM
Lymph node	0.1 nTPM
Colon	0.0 nTPM
Kidney	0.0 nTPM
Liver	0.0 nTPM
PBMC	0.0 nTPM
Adipose tissue	0.0 nTPM
Bone marrow	0.0 nTPM
Stomach	0.0 nTPM

Basal keratinocytes	31.2 nTPM
Skin	31.2 nTPM

Dendritic cells	0.5 nTPM
Breast	1.1 nTPM
Adipose tissue	0.3 nTPM
PBMC	0.0 nTPM

Endothelial cells	115.5 nTPM
Prostate	386.8 nTPM
Skin	342.6 nTPM
Skeletal muscle	264.4 nTPM
Eye	186.1 nTPM
Lung	121.6 nTPM
Esophagus	117.3 nTPM
Ovary	58.6 nTPM
Pancreas	46.4 nTPM
Breast	28.9 nTPM
Liver	19.2 nTPM
Placenta	18.2 nTPM
Endometrium	13.0 nTPM
Heart muscle	10.0 nTPM
Testis	4.4 nTPM

Fibroblasts	88.8 nTPM
Prostate	418.6 nTPM
Stomach	178.2 nTPM
Skeletal muscle	127.0 nTPM
Lung	65.9 nTPM
Skin	64.8 nTPM
Esophagus	49.0 nTPM
Heart muscle	36.2 nTPM
Adipose tissue	12.8 nTPM
Placenta	9.3 nTPM
Breast	8.2 nTPM
Ovary	6.5 nTPM

Intestinal goblet cells	0.0 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM
Rectum	0.0 nTPM

Macrophages	3.2 nTPM
Skeletal muscle	19.4 nTPM
Prostate	12.6 nTPM
Esophagus	6.3 nTPM
Lung	4.6 nTPM
Breast	1.6 nTPM
Spleen	1.0 nTPM
Kidney	0.8 nTPM
Adipose tissue	0.8 nTPM
Testis	0.6 nTPM
Endometrium	0.4 nTPM
Ovary	0.2 nTPM
PBMC	0.0 nTPM
Pancreas	0.0 nTPM
Bone marrow	0.0 nTPM
Stomach	0.0 nTPM

Paneth cells	0.0 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM
Rectum	0.0 nTPM

Plasma cells	0.7 nTPM
Stomach	2.0 nTPM
Bone marrow	1.7 nTPM
Adipose tissue	0.0 nTPM
Esophagus	0.0 nTPM
Spleen	0.0 nTPM

Respiratory ciliated cells	0.0 nTPM
Lung	0.0 nTPM
Bronchus	0.0 nTPM

Smooth muscle cells	313.7 nTPM
Prostate	1309.4 nTPM
Skeletal muscle	886.8 nTPM
Skin	536.8 nTPM
Adipose tissue	259.0 nTPM
Esophagus	203.1 nTPM
Heart muscle	86.2 nTPM
Ovary	61.6 nTPM
Pancreas	42.8 nTPM
Endometrium	37.2 nTPM
Breast	27.3 nTPM
Bronchus	1.0 nTPM

T-cells	3.7 nTPM
Skin	24.4 nTPM
Prostate	9.5 nTPM
Skeletal muscle	8.7 nTPM
Esophagus	6.6 nTPM
Bone marrow	2.5 nTPM
Kidney	2.0 nTPM
Lung	1.5 nTPM
Spleen	1.5 nTPM
Lymph node	1.4 nTPM
Liver	1.3 nTPM
Breast	1.0 nTPM
Endometrium	0.9 nTPM
PBMC	0.6 nTPM
Stomach	0.4 nTPM
Adipose tissue	0.2 nTPM
Colon	0.0 nTPM
Ovary	0.0 nTPM

Undifferentiated cells	0.3 nTPM
Colon	0.7 nTPM
Small intestine	0.0 nTPM
Rectum	0.0 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC