Single cell type - RPS6KA4 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	RPS6KA4
Gene descriptionⁱ Full gene name according to HGNC.	Ribosomal protein S6 kinase A4
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Enzymes Metabolic proteins Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	3

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Cytotrophoblasts - Unknown function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (dendritic cells, Cytotrophoblasts, Pancreatic endocrine cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enhanced (plasmacytoid DC)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (dendritic cells, Cytotrophoblasts, Pancreatic endocrine cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

RPS6KA4 is part of cluster 47 Cytotrophoblasts - Unknown function with confidenceⁱ 1
226 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
MYCL	MYCL proto-oncogene, bHLH transcription factor	0.6878	61
SLC12A9	Solute carrier family 12 member 9	0.6522	47
DPPA4	Developmental pluripotency associated 4	0.6481	47
CD300LB	CD300 molecule like family member b	0.5648	19
P2RY6	Pyrimidinergic receptor P2Y6	0.5489	18
PLD4	Phospholipase D family member 4	0.5379	65
TLCD4-RWDD3	TLCD4-RWDD3 readthrough	0.5341	47
IFI6	Interferon alpha inducible protein 6	0.5299	47
MBNL3	Muscleblind like splicing regulator 3	0.5281	47
MDFI	MyoD family inhibitor	0.5274	47
HPDL	4-hydroxyphenylpyruvate dioxygenase like	0.5249	47
SMIM43	Small integral membrane protein 43	0.5243	47
MAGEA8	MAGE family member A8	0.5228	47
MAB21L4	Mab-21 like 4	0.5197	47
SOX14	SRY-box transcription factor 14	0.5158	47

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	11.8 nTPM
Adipose tissue	12.4 nTPM
Breast	11.2 nTPM

Alveolar cells type 1	3.2 nTPM
Lung	3.2 nTPM

Alveolar cells type 2	3.0 nTPM
Lung	3.0 nTPM

Astrocytes	8.5 nTPM
Brain	8.5 nTPM

B-cells	6.4 nTPM
Stomach	8.6 nTPM
Liver	8.5 nTPM
Lymph node	8.4 nTPM
Spleen	8.3 nTPM
Colon	8.0 nTPM
PBMC	7.1 nTPM
Bone marrow	6.6 nTPM
Breast	5.3 nTPM
Adipose tissue	3.0 nTPM
Kidney	0.0 nTPM

Dendritic cells	75.0 nTPM
PBMC	203.2 nTPM
Breast	14.6 nTPM
Adipose tissue	7.2 nTPM

Endothelial cells	9.9 nTPM
Eye	22.7 nTPM
Ovary	13.1 nTPM
Esophagus	12.4 nTPM
Endometrium	11.0 nTPM
Heart muscle	10.6 nTPM
Placenta	10.2 nTPM
Skeletal muscle	10.2 nTPM
Breast	9.7 nTPM
Liver	8.2 nTPM
Testis	7.7 nTPM
Pancreas	7.7 nTPM
Skin	7.4 nTPM
Prostate	6.9 nTPM
Lung	0.0 nTPM

Fibroblasts	9.3 nTPM
Placenta	28.8 nTPM
Ovary	10.7 nTPM
Skin	10.3 nTPM
Skeletal muscle	9.6 nTPM
Adipose tissue	8.6 nTPM
Breast	7.9 nTPM
Esophagus	7.7 nTPM
Stomach	7.4 nTPM
Heart muscle	4.7 nTPM
Lung	3.4 nTPM
Prostate	3.4 nTPM

Intestinal goblet cells	14.3 nTPM
Colon	20.1 nTPM
Rectum	12.9 nTPM
Small intestine	10.1 nTPM

Macrophages	25.8 nTPM
Breast	50.5 nTPM
Endometrium	42.4 nTPM
Stomach	41.0 nTPM
Spleen	33.2 nTPM
Bone marrow	32.4 nTPM
Testis	32.0 nTPM
Skeletal muscle	27.6 nTPM
Adipose tissue	24.1 nTPM
Ovary	23.2 nTPM
Esophagus	20.4 nTPM
Prostate	19.5 nTPM
Pancreas	17.8 nTPM
Kidney	15.8 nTPM
Lung	7.2 nTPM
PBMC	0.0 nTPM

Paneth cells	12.8 nTPM
Colon	23.1 nTPM
Rectum	11.7 nTPM
Small intestine	3.7 nTPM

Plasma cells	5.7 nTPM
Spleen	7.8 nTPM
Stomach	6.3 nTPM
Esophagus	6.1 nTPM
Adipose tissue	5.2 nTPM
Bone marrow	3.3 nTPM

Respiratory ciliated cells	3.1 nTPM
Bronchus	6.2 nTPM
Lung	0.0 nTPM

Smooth muscle cells	10.2 nTPM
Pancreas	18.3 nTPM
Bronchus	15.1 nTPM
Ovary	12.3 nTPM
Skin	11.2 nTPM
Skeletal muscle	9.4 nTPM
Breast	9.4 nTPM
Endometrium	8.9 nTPM
Adipose tissue	7.7 nTPM
Prostate	7.2 nTPM
Heart muscle	6.8 nTPM
Esophagus	6.2 nTPM

T-cells	11.3 nTPM
Skeletal muscle	17.4 nTPM
Liver	16.0 nTPM
Stomach	15.8 nTPM
Breast	14.5 nTPM
Colon	13.8 nTPM
Spleen	13.2 nTPM
Esophagus	13.1 nTPM
PBMC	12.8 nTPM
Bone marrow	12.6 nTPM
Lymph node	12.2 nTPM
Ovary	11.1 nTPM
Skin	9.7 nTPM
Lung	9.1 nTPM
Kidney	7.5 nTPM
Endometrium	7.3 nTPM
Adipose tissue	6.0 nTPM
Prostate	0.0 nTPM

Basal keratinocytes	12.1 nTPM
Skin	12.1 nTPM

Cone photoreceptor cells	4.2 nTPM
Eye	4.2 nTPM

Distal enterocytes	25.2 nTPM
Colon	26.8 nTPM
Rectum	23.6 nTPM

Distal tubular cells	5.9 nTPM
Kidney	5.9 nTPM

Undifferentiated cells	19.9 nTPM
Colon	27.5 nTPM
Rectum	17.9 nTPM
Small intestine	14.3 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC