Single cell type - RNF168 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	RNF168
Gene descriptionⁱ Full gene name according to HGNC.	Ring finger protein 168
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Enzymes Human disease related genes Potential drug targets
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	1

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Spermatids - Spermatogenesis (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Late spermatids, Early spermatids)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Late spermatids, Early spermatids)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

RNF168 is part of cluster 25 Spermatids - Spermatogenesis with confidenceⁱ 1
458 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
BNC1	Basonuclin 1	0.7044	25
SLC10A6	Solute carrier family 10 member 6	0.6212	25
LCTL	Lactase like	0.6097	25
RANGAP1	Ran GTPase activating protein 1	0.5961	25
CCDC187	Coiled-coil domain containing 187	0.5841	25
UBE2N	Ubiquitin conjugating enzyme E2 N	0.5838	25
NUP35	Nucleoporin 35	0.5834	25
SPATA18	Spermatogenesis associated 18	0.5821	25
CUL9	Cullin 9	0.5815	25
PTP4A1	Protein tyrosine phosphatase 4A1	0.5781	25
KIF2C	Kinesin family member 2C	0.5757	25
CSNK1G2	Casein kinase 1 gamma 2	0.5747	25
TLE3	TLE family member 3, transcriptional corepressor	0.5741	28
DGCR6L	DiGeorge syndrome critical region gene 6 like	0.5701	25
LRTOMT	Leucine rich transmembrane and O-methyltransferase domain containing	0.5665	25

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	34.4 nTPM
Breast	36.0 nTPM
Adipose tissue	32.9 nTPM

Alveolar cells type 1	16.0 nTPM
Lung	16.0 nTPM

Alveolar cells type 2	37.5 nTPM
Lung	37.5 nTPM

Astrocytes	16.0 nTPM
Brain	16.0 nTPM

B-cells	37.7 nTPM
Adipose tissue	64.7 nTPM
Colon	62.3 nTPM
Stomach	45.4 nTPM
Lymph node	39.6 nTPM
Breast	37.5 nTPM
Spleen	36.7 nTPM
PBMC	29.9 nTPM
Liver	26.6 nTPM
Kidney	19.0 nTPM
Bone marrow	15.5 nTPM

Dendritic cells	76.4 nTPM
Adipose tissue	114.5 nTPM
Breast	98.8 nTPM
PBMC	16.0 nTPM

Endothelial cells	33.3 nTPM
Skin	70.7 nTPM
Heart muscle	46.2 nTPM
Prostate	38.5 nTPM
Endometrium	38.4 nTPM
Esophagus	34.6 nTPM
Liver	32.1 nTPM
Lung	31.2 nTPM
Skeletal muscle	30.8 nTPM
Ovary	30.3 nTPM
Testis	26.9 nTPM
Breast	26.8 nTPM
Eye	23.5 nTPM
Pancreas	19.3 nTPM
Placenta	17.0 nTPM

Fibroblasts	27.9 nTPM
Skin	69.0 nTPM
Lung	39.1 nTPM
Prostate	38.0 nTPM
Esophagus	23.7 nTPM
Skeletal muscle	23.6 nTPM
Breast	23.2 nTPM
Heart muscle	20.7 nTPM
Ovary	19.6 nTPM
Stomach	19.3 nTPM
Adipose tissue	18.6 nTPM
Placenta	12.6 nTPM

Intestinal goblet cells	18.5 nTPM
Colon	22.1 nTPM
Rectum	17.6 nTPM
Small intestine	15.8 nTPM

Macrophages	33.9 nTPM
Lung	53.2 nTPM
Adipose tissue	50.0 nTPM
Spleen	42.5 nTPM
Skeletal muscle	40.9 nTPM
Pancreas	38.3 nTPM
Breast	38.3 nTPM
Ovary	38.0 nTPM
Stomach	35.1 nTPM
Kidney	34.8 nTPM
Esophagus	33.1 nTPM
Endometrium	32.8 nTPM
Prostate	31.0 nTPM
Testis	23.2 nTPM
Bone marrow	17.5 nTPM
PBMC	0.0 nTPM

Paneth cells	17.3 nTPM
Rectum	20.4 nTPM
Colon	16.5 nTPM
Small intestine	15.0 nTPM

Plasma cells	37.9 nTPM
Adipose tissue	79.3 nTPM
Spleen	40.0 nTPM
Stomach	33.5 nTPM
Bone marrow	24.7 nTPM
Esophagus	12.3 nTPM

Respiratory ciliated cells	17.1 nTPM
Lung	20.4 nTPM
Bronchus	13.9 nTPM

Smooth muscle cells	25.7 nTPM
Heart muscle	49.1 nTPM
Skin	48.5 nTPM
Prostate	36.9 nTPM
Breast	26.8 nTPM
Endometrium	23.5 nTPM
Esophagus	20.1 nTPM
Ovary	19.8 nTPM
Skeletal muscle	18.0 nTPM
Pancreas	16.9 nTPM
Adipose tissue	15.8 nTPM
Bronchus	7.7 nTPM

T-cells	63.7 nTPM
Colon	115.9 nTPM
Prostate	113.7 nTPM
Skin	107.2 nTPM
Adipose tissue	86.3 nTPM
Breast	79.1 nTPM
Endometrium	74.1 nTPM
Skeletal muscle	74.1 nTPM
Esophagus	52.4 nTPM
Ovary	48.9 nTPM
Kidney	48.6 nTPM
Lung	48.6 nTPM
Lymph node	47.3 nTPM
PBMC	42.1 nTPM
Spleen	42.0 nTPM
Stomach	37.9 nTPM
Liver	36.9 nTPM
Bone marrow	27.7 nTPM

Basal keratinocytes	100.1 nTPM
Skin	100.1 nTPM

Basal prostatic cells	49.8 nTPM
Prostate	49.8 nTPM

Basal squamous epithelial cells	52.3 nTPM
Esophagus	52.3 nTPM

Cholangiocytes	12.8 nTPM
Liver	12.8 nTPM

Undifferentiated cells	22.4 nTPM
Colon	26.2 nTPM
Rectum	22.4 nTPM
Small intestine	18.7 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC