Single cell type - CKB - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	CKB
Gene descriptionⁱ Full gene name according to HGNC.	Creatine kinase B
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Cancer-related genes Candidate cardiovascular disease genes Enzymes FDA approved drug targets Metabolic proteins Plasma proteins
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	5

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Intestinal epithelial cells - Unknown function (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Horizontal cells, Distal enterocytes, Rod photoreceptor cells, Muller glia cells, Bipolar cells, Distal tubular cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enriched (non-classical monocyte)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (CACO-2, HEK93, SCLC-21H, SH-SY5Y)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Horizontal cells, Distal enterocytes, Rod photoreceptor cells, Muller glia cells, Bipolar cells, Distal tubular cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

CKB is part of cluster 53 Intestinal epithelial cells - Unknown function with confidenceⁱ 1
611 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
TPRG1L	Tumor protein p63 regulated 1 like	0.6605	62
NR2F6	Nuclear receptor subfamily 2 group F member 6	0.6415	53
PIGZ	Phosphatidylinositol glycan anchor biosynthesis class Z	0.6265	53
HOXA13	Homeobox A13	0.6189	53
C8orf33	Chromosome 8 open reading frame 33	0.6131	53
ITM2C	Integral membrane protein 2C	0.6087	53
FXYD3	FXYD domain containing ion transport regulator 3	0.6083	53
CNNM4	Cyclin and CBS domain divalent metal cation transport mediator 4	0.6075	53
FZD5	Frizzled class receptor 5	0.6067	53
HEPH	Hephaestin	0.5870	53
PADI2	Peptidyl arginine deiminase 2	0.5823	53
AP3S2	Adaptor related protein complex 3 subunit sigma 2	0.5793	53
CLDN8	Claudin 8	0.5729	53
RIMKLA	Ribosomal modification protein rimK like family member A	0.5725	53
PARM1	Prostate androgen-regulated mucin-like protein 1	0.5662	53

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	14.8 nTPM
Breast	21.7 nTPM
Adipose tissue	7.9 nTPM

Alveolar cells type 1	195.5 nTPM
Lung	195.5 nTPM

Alveolar cells type 2	103.5 nTPM
Lung	103.5 nTPM

Astrocytes	515.8 nTPM
Brain	515.8 nTPM

B-cells	13.5 nTPM
Colon	63.9 nTPM
Breast	29.4 nTPM
Kidney	23.8 nTPM
Stomach	9.1 nTPM
Liver	4.6 nTPM
Lymph node	1.6 nTPM
Adipose tissue	1.5 nTPM
Spleen	1.4 nTPM
PBMC	0.0 nTPM
Bone marrow	0.0 nTPM

Dendritic cells	3.4 nTPM
Breast	7.2 nTPM
Adipose tissue	3.0 nTPM
PBMC	0.0 nTPM

Distal enterocytes	4691.8 nTPM
Rectum	7807.6 nTPM
Colon	1576.1 nTPM

Endothelial cells	60.8 nTPM
Eye	463.6 nTPM
Heart muscle	119.8 nTPM
Liver	89.9 nTPM
Testis	43.8 nTPM
Endometrium	33.0 nTPM
Skin	29.6 nTPM
Prostate	28.4 nTPM
Breast	19.3 nTPM
Skeletal muscle	7.4 nTPM
Lung	6.2 nTPM
Esophagus	4.6 nTPM
Placenta	2.9 nTPM
Ovary	2.1 nTPM
Pancreas	0.0 nTPM

Enteroendocrine cells	467.1 nTPM
Rectum	681.9 nTPM
Colon	252.2 nTPM

Fibroblasts	96.6 nTPM
Heart muscle	218.2 nTPM
Prostate	189.9 nTPM
Skin	121.8 nTPM
Breast	113.1 nTPM
Adipose tissue	81.8 nTPM
Stomach	72.7 nTPM
Placenta	66.8 nTPM
Lung	64.8 nTPM
Esophagus	56.9 nTPM
Skeletal muscle	48.7 nTPM
Ovary	27.8 nTPM

Intestinal goblet cells	726.0 nTPM
Rectum	1206.0 nTPM
Colon	884.1 nTPM
Small intestine	88.0 nTPM

Macrophages	39.8 nTPM
Breast	163.4 nTPM
Pancreas	137.9 nTPM
Prostate	74.5 nTPM
Endometrium	50.3 nTPM
Kidney	45.3 nTPM
Adipose tissue	27.6 nTPM
Ovary	19.8 nTPM
Esophagus	17.3 nTPM
Testis	16.3 nTPM
Skeletal muscle	15.0 nTPM
Stomach	11.7 nTPM
Lung	11.6 nTPM
Spleen	4.2 nTPM
Bone marrow	2.5 nTPM
PBMC	0.0 nTPM

Paneth cells	1996.9 nTPM
Colon	3069.8 nTPM
Rectum	2275.2 nTPM
Small intestine	645.8 nTPM

Plasma cells	6.6 nTPM
Stomach	17.9 nTPM
Esophagus	6.1 nTPM
Adipose tissue	5.8 nTPM
Bone marrow	1.7 nTPM
Spleen	1.3 nTPM

Respiratory ciliated cells	456.1 nTPM
Bronchus	584.4 nTPM
Lung	327.9 nTPM

Basal keratinocytes	153.7 nTPM
Skin	153.7 nTPM

Basal prostatic cells	296.2 nTPM
Prostate	296.2 nTPM

Basal respiratory cells	257.6 nTPM
Bronchus	257.6 nTPM

Cholangiocytes	145.8 nTPM
Liver	145.8 nTPM

Smooth muscle cells	222.6 nTPM
Prostate	540.7 nTPM
Esophagus	339.5 nTPM
Breast	335.5 nTPM
Ovary	267.5 nTPM
Skin	223.9 nTPM
Endometrium	223.0 nTPM
Heart muscle	213.5 nTPM
Bronchus	144.2 nTPM
Skeletal muscle	86.4 nTPM
Adipose tissue	60.0 nTPM
Pancreas	14.0 nTPM

T-cells	19.1 nTPM
Colon	91.2 nTPM
Endometrium	65.4 nTPM
Prostate	42.6 nTPM
Skin	37.8 nTPM
Lung	15.2 nTPM
Kidney	13.1 nTPM
Esophagus	13.1 nTPM
Breast	11.9 nTPM
Adipose tissue	7.6 nTPM
Stomach	7.2 nTPM
Ovary	6.7 nTPM
Skeletal muscle	6.5 nTPM
Liver	3.6 nTPM
PBMC	1.4 nTPM
Spleen	0.3 nTPM
Lymph node	0.2 nTPM
Bone marrow	0.1 nTPM

Undifferentiated cells	755.6 nTPM
Rectum	1391.0 nTPM
Colon	852.7 nTPM
Small intestine	23.0 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC