Single cell type - API5 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	API5
Gene descriptionⁱ Full gene name according to HGNC.	Apoptosis inhibitor 5
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	7

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Non-specific - Transcription (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low cell type specificity
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Low cell line specificity
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in all
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Low cell type specificity

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

API5 is part of cluster 59 Non-specific - Transcription with confidenceⁱ 1
354 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
NUP133	Nucleoporin 133	0.6833	59
RRN3	RRN3 homolog, RNA polymerase I transcription factor	0.6463	59
HMGN4	High mobility group nucleosomal binding domain 4	0.6445	59
ZNF484	Zinc finger protein 484	0.6434	59
RWDD3	RWD domain containing 3	0.6363	59
C5orf22	Chromosome 5 open reading frame 22	0.6271	59
NHEJ1	Non-homologous end joining factor 1	0.6207	59
WDR36	WD repeat domain 36	0.5962	59
SLC25A32	Solute carrier family 25 member 32	0.5933	59
ZNF410	Zinc finger protein 410	0.5832	59
MAT2B	Methionine adenosyltransferase 2B	0.5788	59
MMGT1	Membrane magnesium transporter 1	0.5769	59
DMRTC1	DMRT like family C1	0.5749	59
C5orf51	Chromosome 5 open reading frame 51	0.5730	59
NBPF15	NBPF member 15	0.5714	43

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	26.4 nTPM
Adipose tissue	29.9 nTPM
Breast	22.8 nTPM

Alveolar cells type 1	16.0 nTPM
Lung	16.0 nTPM

Alveolar cells type 2	27.3 nTPM
Lung	27.3 nTPM

Astrocytes	30.0 nTPM
Brain	30.0 nTPM

B-cells	35.9 nTPM
Spleen	60.9 nTPM
Lymph node	59.0 nTPM
Stomach	58.5 nTPM
Bone marrow	56.4 nTPM
PBMC	27.1 nTPM
Breast	25.5 nTPM
Liver	24.3 nTPM
Adipose tissue	20.1 nTPM
Colon	17.6 nTPM
Kidney	9.5 nTPM

Dendritic cells	31.8 nTPM
Adipose tissue	32.1 nTPM
PBMC	31.8 nTPM
Breast	31.3 nTPM

Endothelial cells	26.2 nTPM
Esophagus	45.4 nTPM
Heart muscle	41.0 nTPM
Lung	31.2 nTPM
Skin	30.3 nTPM
Endometrium	27.0 nTPM
Ovary	25.6 nTPM
Eye	25.1 nTPM
Prostate	24.4 nTPM
Skeletal muscle	24.0 nTPM
Liver	23.8 nTPM
Testis	23.7 nTPM
Breast	22.2 nTPM
Pancreas	11.6 nTPM
Placenta	11.3 nTPM

Fibroblasts	28.3 nTPM
Stomach	46.0 nTPM
Heart muscle	36.8 nTPM
Esophagus	34.3 nTPM
Skin	31.2 nTPM
Ovary	28.1 nTPM
Lung	27.9 nTPM
Adipose tissue	26.0 nTPM
Skeletal muscle	23.9 nTPM
Prostate	23.7 nTPM
Breast	20.2 nTPM
Placenta	13.5 nTPM

Intestinal goblet cells	27.5 nTPM
Rectum	34.1 nTPM
Small intestine	29.1 nTPM
Colon	19.3 nTPM

Macrophages	30.0 nTPM
Spleen	51.8 nTPM
Esophagus	48.7 nTPM
Bone marrow	41.2 nTPM
Kidney	33.3 nTPM
Skeletal muscle	31.3 nTPM
Lung	30.7 nTPM
Ovary	30.6 nTPM
PBMC	27.3 nTPM
Adipose tissue	26.1 nTPM
Testis	25.0 nTPM
Endometrium	24.3 nTPM
Stomach	23.5 nTPM
Pancreas	23.0 nTPM
Breast	22.3 nTPM
Prostate	11.5 nTPM

Paneth cells	19.8 nTPM
Rectum	28.1 nTPM
Colon	16.5 nTPM
Small intestine	15.0 nTPM

Plasma cells	44.2 nTPM
Bone marrow	65.9 nTPM
Spleen	60.5 nTPM
Stomach	56.1 nTPM
Adipose tissue	20.0 nTPM
Esophagus	18.4 nTPM

Respiratory ciliated cells	20.8 nTPM
Bronchus	21.3 nTPM
Lung	20.4 nTPM

Smooth muscle cells	31.1 nTPM
Esophagus	54.3 nTPM
Skin	37.0 nTPM
Heart muscle	35.9 nTPM
Bronchus	35.7 nTPM
Ovary	27.8 nTPM
Skeletal muscle	27.0 nTPM
Adipose tissue	27.0 nTPM
Pancreas	26.4 nTPM
Prostate	24.7 nTPM
Breast	23.1 nTPM
Endometrium	22.8 nTPM

T-cells	33.8 nTPM
Stomach	63.7 nTPM
Spleen	59.2 nTPM
Bone marrow	54.8 nTPM
Lymph node	53.4 nTPM
Skin	40.6 nTPM
Kidney	36.9 nTPM
PBMC	33.9 nTPM
Skeletal muscle	32.1 nTPM
Lung	30.4 nTPM
Adipose tissue	30.2 nTPM
Liver	28.4 nTPM
Breast	26.8 nTPM
Endometrium	24.2 nTPM
Esophagus	19.7 nTPM
Ovary	17.8 nTPM
Colon	17.6 nTPM
Prostate	4.7 nTPM

Basal keratinocytes	43.8 nTPM
Skin	43.8 nTPM

Basal prostatic cells	16.6 nTPM
Prostate	16.6 nTPM

Basal respiratory cells	21.6 nTPM
Bronchus	21.6 nTPM

Basal squamous epithelial cells	47.3 nTPM
Esophagus	47.3 nTPM

Undifferentiated cells	31.4 nTPM
Small intestine	33.8 nTPM
Rectum	33.5 nTPM
Colon	26.8 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC