Single cell type - ZNF296 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ZNF296
Gene descriptionⁱ Full gene name according to HGNC.	Zinc finger protein 296
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Transcription factors
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	2

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	B-cells - Immune response (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Squamous epithelial cells, Basal squamous epithelial cells, B-cells, Plasma cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (Daudi, SK-BR-3, U-698)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Squamous epithelial cells, Basal squamous epithelial cells, B-cells, Plasma cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

ZNF296 is part of cluster 57 B-cells - Immune response with confidenceⁱ 1
204 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
IGKV2D-26	Immunoglobulin kappa variable 2D-26	0.7058	57
BRD2	Bromodomain containing 2	0.7037	7
IGLV3-16	Immunoglobulin lambda variable 3-16	0.6970	57
ELL3	Elongation factor for RNA polymerase II 3	0.6517	61
CD19	CD19 molecule	0.6464	57
SRSF2	Serine and arginine rich splicing factor 2	0.6456	7
IGHV1-58	Immunoglobulin heavy variable 1-58	0.6424	57
FCRL1	Fc receptor like 1	0.6393	57
FCRL2	Fc receptor like 2	0.6392	57
CXCR5	C-X-C motif chemokine receptor 5	0.6350	57
CR2	Complement C3d receptor 2	0.6345	57
PAX5	Paired box 5	0.6339	57
BANK1	B cell scaffold protein with ankyrin repeats 1	0.6291	57
PPP1R10	Protein phosphatase 1 regulatory subunit 10	0.6283	7
TLR10	Toll like receptor 10	0.6249	57

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	2.4 nTPM
Breast	3.5 nTPM
Adipose tissue	1.3 nTPM

Alveolar cells type 1	0.0 nTPM
Lung	0.0 nTPM

Alveolar cells type 2	0.6 nTPM
Lung	0.6 nTPM

Astrocytes	2.3 nTPM
Brain	2.3 nTPM

Basal keratinocytes	12.1 nTPM
Skin	12.1 nTPM

B-cells	53.3 nTPM
Lymph node	169.8 nTPM
Breast	97.7 nTPM
Stomach	52.6 nTPM
PBMC	47.1 nTPM
Spleen	43.3 nTPM
Liver	35.1 nTPM
Adipose tissue	30.0 nTPM
Colon	25.5 nTPM
Bone marrow	17.2 nTPM
Kidney	14.3 nTPM

Dendritic cells	31.9 nTPM
Breast	49.9 nTPM
PBMC	31.8 nTPM
Adipose tissue	13.8 nTPM

Endothelial cells	2.0 nTPM
Esophagus	9.3 nTPM
Skin	7.2 nTPM
Breast	4.8 nTPM
Prostate	2.3 nTPM
Heart muscle	2.0 nTPM
Skeletal muscle	1.2 nTPM
Endometrium	0.7 nTPM
Ovary	0.2 nTPM
Eye	0.0 nTPM
Liver	0.0 nTPM
Lung	0.0 nTPM
Placenta	0.0 nTPM
Testis	0.0 nTPM
Pancreas	0.0 nTPM

Fibroblasts	1.3 nTPM
Skin	5.5 nTPM
Breast	2.0 nTPM
Prostate	1.9 nTPM
Esophagus	1.6 nTPM
Lung	1.2 nTPM
Placenta	0.8 nTPM
Ovary	0.5 nTPM
Skeletal muscle	0.4 nTPM
Adipose tissue	0.2 nTPM
Heart muscle	0.1 nTPM
Stomach	0.0 nTPM

Intestinal goblet cells	32.1 nTPM
Colon	57.2 nTPM
Rectum	24.2 nTPM
Small intestine	15.0 nTPM

Macrophages	7.6 nTPM
Breast	33.3 nTPM
Esophagus	28.3 nTPM
Adipose tissue	8.8 nTPM
Endometrium	8.2 nTPM
Testis	5.2 nTPM
Skeletal muscle	5.1 nTPM
Kidney	4.6 nTPM
Prostate	4.6 nTPM
Bone marrow	3.7 nTPM
Ovary	3.6 nTPM
Spleen	3.1 nTPM
Pancreas	2.5 nTPM
Lung	2.3 nTPM
PBMC	0.0 nTPM
Stomach	0.0 nTPM

Paneth cells	36.1 nTPM
Small intestine	60.1 nTPM
Rectum	31.7 nTPM
Colon	16.5 nTPM

Plasma cells	48.2 nTPM
Esophagus	135.4 nTPM
Spleen	44.0 nTPM
Stomach	43.6 nTPM
Adipose tissue	9.7 nTPM
Bone marrow	8.2 nTPM

Respiratory ciliated cells	3.9 nTPM
Bronchus	4.8 nTPM
Lung	2.9 nTPM

Smooth muscle cells	3.2 nTPM
Skin	10.2 nTPM
Esophagus	7.8 nTPM
Breast	5.7 nTPM
Adipose tissue	3.9 nTPM
Prostate	2.2 nTPM
Pancreas	1.4 nTPM
Endometrium	1.3 nTPM
Skeletal muscle	1.2 nTPM
Bronchus	1.0 nTPM
Heart muscle	0.5 nTPM
Ovary	0.5 nTPM

Cone photoreceptor cells	4.2 nTPM
Eye	4.2 nTPM

Distal enterocytes	18.5 nTPM
Colon	30.2 nTPM
Rectum	6.8 nTPM

Exocrine glandular cells	0.9 nTPM
Pancreas	0.9 nTPM

T-cells	18.5 nTPM
Esophagus	72.0 nTPM
Breast	62.5 nTPM
Lymph node	26.3 nTPM
Skin	22.6 nTPM
Colon	16.1 nTPM
Ovary	15.6 nTPM
Adipose tissue	15.1 nTPM
Spleen	13.9 nTPM
Liver	13.5 nTPM
Skeletal muscle	11.6 nTPM
Endometrium	11.5 nTPM
Prostate	9.5 nTPM
Stomach	9.4 nTPM
Lung	4.5 nTPM
PBMC	3.9 nTPM
Bone marrow	3.9 nTPM
Kidney	2.0 nTPM

Undifferentiated cells	28.9 nTPM
Colon	36.5 nTPM
Rectum	26.3 nTPM
Small intestine	23.8 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC