Single cell type - AGR3 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	AGR3
Gene descriptionⁱ Full gene name according to HGNC.	Anterior gradient 3, protein disulphide isomerase family member
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	3

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Ciliated cells - Cilium organization (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Respiratory ciliated cells, Proximal enterocytes, Endometrial ciliated cells, Paneth cells, Intestinal goblet cells, Undifferentiated cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Not detected in immune cells
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Not detected
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Group enriched (OE19, T-47d)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in some
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Respiratory ciliated cells, Proximal enterocytes, Endometrial ciliated cells, Paneth cells, Intestinal goblet cells, Undifferentiated cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

AGR3 is part of cluster 49 Ciliated cells - Cilium organization with confidenceⁱ 1
457 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
TCTEX1D4	Tctex1 domain containing 4	0.7631	49
CCDC78	Coiled-coil domain containing 78	0.7440	49
FAM166B	Family with sequence similarity 166 member B	0.7362	49
GAS2L2	Growth arrest specific 2 like 2	0.7294	49
CCDC153	Coiled-coil domain containing 153	0.7216	49
CDHR4	Cadherin related family member 4	0.7127	49
CCDC17	Coiled-coil domain containing 17	0.7108	49
WDR38	WD repeat domain 38	0.7066	49
OMG	Oligodendrocyte myelin glycoprotein	0.7009	49
CD164L2	CD164 molecule like 2	0.6975	49
TMC5	Transmembrane channel like 5	0.6914	49
STMND1	Stathmin domain containing 1	0.6888	49
RSPH1	Radial spoke head component 1	0.6858	49
ANKRD66	Ankyrin repeat domain 66	0.6786	49
MAPK15	Mitogen-activated protein kinase 15	0.6782	49

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	0.7 nTPM
Breast	1.4 nTPM
Adipose tissue	0.0 nTPM

Alveolar cells type 1	333.3 nTPM
Lung	333.3 nTPM

Alveolar cells type 2	408.1 nTPM
Lung	408.1 nTPM

Astrocytes	0.3 nTPM
Brain	0.3 nTPM

B-cells	1.4 nTPM
Colon	12.8 nTPM
Stomach	0.9 nTPM
Kidney	0.0 nTPM
Liver	0.0 nTPM
PBMC	0.0 nTPM
Adipose tissue	0.0 nTPM
Lymph node	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM
Breast	0.0 nTPM

Dendritic cells	0.7 nTPM
Breast	2.2 nTPM
PBMC	0.0 nTPM
Adipose tissue	0.0 nTPM

Endothelial cells	1.9 nTPM
Lung	15.6 nTPM
Breast	7.9 nTPM
Endometrium	2.3 nTPM
Prostate	0.7 nTPM
Skin	0.3 nTPM
Eye	0.0 nTPM
Heart muscle	0.0 nTPM
Liver	0.0 nTPM
Placenta	0.0 nTPM
Testis	0.0 nTPM
Pancreas	0.0 nTPM
Skeletal muscle	0.0 nTPM
Ovary	0.0 nTPM
Esophagus	0.0 nTPM

Enteroendocrine cells	112.7 nTPM
Colon	113.7 nTPM
Rectum	111.6 nTPM

Fibroblasts	5.3 nTPM
Lung	54.7 nTPM
Breast	1.7 nTPM
Prostate	1.2 nTPM
Placenta	1.1 nTPM
Skin	0.1 nTPM
Heart muscle	0.0 nTPM
Skeletal muscle	0.0 nTPM
Ovary	0.0 nTPM
Adipose tissue	0.0 nTPM
Esophagus	0.0 nTPM
Stomach	0.0 nTPM

Intestinal goblet cells	701.9 nTPM
Colon	1025.6 nTPM
Small intestine	584.2 nTPM
Rectum	496.0 nTPM

Macrophages	2.3 nTPM
Lung	21.9 nTPM
Endometrium	6.2 nTPM
Pancreas	2.5 nTPM
Breast	2.3 nTPM
Prostate	1.2 nTPM
Kidney	0.0 nTPM
PBMC	0.0 nTPM
Testis	0.0 nTPM
Skeletal muscle	0.0 nTPM
Ovary	0.0 nTPM
Adipose tissue	0.0 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM
Stomach	0.0 nTPM

Paneth cells	744.5 nTPM
Small intestine	1208.9 nTPM
Colon	599.4 nTPM
Rectum	425.1 nTPM

Plasma cells	0.4 nTPM
Stomach	1.7 nTPM
Adipose tissue	0.0 nTPM
Esophagus	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM

Respiratory ciliated cells	1771.2 nTPM
Bronchus	2140.3 nTPM
Lung	1402.2 nTPM

Smooth muscle cells	27.9 nTPM
Bronchus	299.6 nTPM
Breast	2.8 nTPM
Endometrium	2.6 nTPM
Prostate	1.3 nTPM
Pancreas	0.3 nTPM
Skin	0.3 nTPM
Heart muscle	0.1 nTPM
Skeletal muscle	0.0 nTPM
Ovary	0.0 nTPM
Adipose tissue	0.0 nTPM
Esophagus	0.0 nTPM

Basal keratinocytes	2.7 nTPM
Skin	2.7 nTPM

Basal prostatic cells	12.5 nTPM
Prostate	12.5 nTPM

Basal respiratory cells	108.5 nTPM
Bronchus	108.5 nTPM

Cholangiocytes	31.5 nTPM
Liver	31.5 nTPM

T-cells	4.1 nTPM
Lung	33.4 nTPM
Colon	18.5 nTPM
Endometrium	15.0 nTPM
Breast	2.2 nTPM
Skin	0.5 nTPM
Stomach	0.5 nTPM
Kidney	0.0 nTPM
Liver	0.0 nTPM
Prostate	0.0 nTPM
PBMC	0.0 nTPM
Skeletal muscle	0.0 nTPM
Ovary	0.0 nTPM
Adipose tissue	0.0 nTPM
Esophagus	0.0 nTPM
Lymph node	0.0 nTPM
Bone marrow	0.0 nTPM
Spleen	0.0 nTPM

Undifferentiated cells	495.4 nTPM
Colon	641.2 nTPM
Small intestine	484.9 nTPM
Rectum	359.9 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC