Single cell type - ORAI3 - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	ORAI3
Gene descriptionⁱ Full gene name according to HGNC.	ORAI calcium release-activated calcium modulator 3
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Transporters
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	3

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Macrophages - Innate immune response (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Hofbauer cells, Hepatocytes)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Low immune cell specificity
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in all
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (HHSteC)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Hofbauer cells, Hepatocytes)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

ORAI3 is part of cluster 4 Macrophages - Innate immune response with confidenceⁱ 1
228 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
TMEM42	Transmembrane protein 42	0.5557	34
GLMP	Glycosylated lysosomal membrane protein	0.5059	4
PLD3	Phospholipase D family member 3	0.5051	4
WDR81	WD repeat domain 81	0.4896	4
RAB3IL1	RAB3A interacting protein like 1	0.4782	34
CTNS	Cystinosin, lysosomal cystine transporter	0.4767	4
HFE	Homeostatic iron regulator	0.4754	4
RAB42	RAB42, member RAS oncogene family	0.4687	4
GBGT1	Globoside alpha-1,3-N-acetylgalactosaminyltransferase 1 (FORS blood group)	0.4509	4
CMKLR1	Chemerin chemokine-like receptor 1	0.4312	4
ADORA3	Adenosine A3 receptor	0.4299	4
RNF135	Ring finger protein 135	0.4290	4
COQ5	Coenzyme Q5, methyltransferase	0.4265	39
TMIGD3	Transmembrane and immunoglobulin domain containing 3	0.4257	4
EPHX1	Epoxide hydrolase 1	0.4250	2

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	12.1 nTPM
Breast	14.6 nTPM
Adipose tissue	9.6 nTPM

Alveolar cells type 1	6.4 nTPM
Lung	6.4 nTPM

Alveolar cells type 2	10.8 nTPM
Lung	10.8 nTPM

Astrocytes	0.0 nTPM
Brain	0.0 nTPM

B-cells	23.9 nTPM
Kidney	47.6 nTPM
PBMC	38.6 nTPM
Stomach	29.0 nTPM
Bone marrow	20.4 nTPM
Colon	19.2 nTPM
Spleen	18.3 nTPM
Lymph node	17.9 nTPM
Adipose tissue	17.1 nTPM
Liver	16.2 nTPM
Breast	14.7 nTPM

Dendritic cells	25.9 nTPM
PBMC	55.8 nTPM
Breast	12.8 nTPM
Adipose tissue	9.2 nTPM

Endothelial cells	16.5 nTPM
Liver	37.6 nTPM
Placenta	30.7 nTPM
Eye	28.3 nTPM
Testis	23.1 nTPM
Ovary	20.8 nTPM
Breast	17.9 nTPM
Esophagus	15.8 nTPM
Prostate	13.5 nTPM
Skeletal muscle	10.7 nTPM
Skin	10.0 nTPM
Endometrium	8.6 nTPM
Lung	6.2 nTPM
Pancreas	3.9 nTPM
Heart muscle	3.7 nTPM

Fibroblasts	25.9 nTPM
Placenta	50.5 nTPM
Esophagus	46.1 nTPM
Adipose tissue	42.0 nTPM
Skeletal muscle	39.8 nTPM
Stomach	22.3 nTPM
Breast	20.7 nTPM
Prostate	18.2 nTPM
Skin	14.0 nTPM
Ovary	13.7 nTPM
Heart muscle	9.9 nTPM
Lung	7.8 nTPM

Intestinal goblet cells	11.3 nTPM
Small intestine	15.7 nTPM
Rectum	13.8 nTPM
Colon	4.3 nTPM

Macrophages	31.4 nTPM
Ovary	94.8 nTPM
Spleen	41.4 nTPM
Kidney	40.1 nTPM
Bone marrow	38.8 nTPM
Pancreas	38.3 nTPM
Esophagus	31.5 nTPM
Skeletal muscle	28.1 nTPM
Prostate	27.5 nTPM
Testis	27.4 nTPM
Stomach	26.4 nTPM
Breast	25.8 nTPM
Endometrium	21.2 nTPM
Adipose tissue	18.4 nTPM
Lung	11.8 nTPM
PBMC	0.0 nTPM

Paneth cells	18.4 nTPM
Small intestine	30.0 nTPM
Rectum	18.6 nTPM
Colon	6.5 nTPM

Plasma cells	18.1 nTPM
Stomach	41.9 nTPM
Esophagus	24.6 nTPM
Bone marrow	9.9 nTPM
Spleen	8.5 nTPM
Adipose tissue	5.8 nTPM

Respiratory ciliated cells	14.7 nTPM
Bronchus	20.7 nTPM
Lung	8.7 nTPM

Smooth muscle cells	14.0 nTPM
Pancreas	20.1 nTPM
Esophagus	20.1 nTPM
Adipose tissue	18.5 nTPM
Ovary	18.3 nTPM
Prostate	16.8 nTPM
Skeletal muscle	14.7 nTPM
Bronchus	12.2 nTPM
Breast	10.1 nTPM
Endometrium	9.3 nTPM
Skin	7.0 nTPM
Heart muscle	6.6 nTPM

T-cells	9.7 nTPM
Liver	23.7 nTPM
Prostate	19.0 nTPM
Kidney	15.1 nTPM
Esophagus	13.1 nTPM
PBMC	11.9 nTPM
Colon	10.5 nTPM
Endometrium	10.1 nTPM
Bone marrow	9.2 nTPM
Breast	9.0 nTPM
Skeletal muscle	7.2 nTPM
Lymph node	7.2 nTPM
Stomach	6.8 nTPM
Spleen	5.2 nTPM
Skin	5.0 nTPM
Ovary	4.5 nTPM
Adipose tissue	4.4 nTPM
Lung	3.0 nTPM

Basal keratinocytes	7.9 nTPM
Skin	7.9 nTPM

Basal prostatic cells	24.5 nTPM
Prostate	24.5 nTPM

Basal respiratory cells	25.6 nTPM
Bronchus	25.6 nTPM

Basal squamous epithelial cells	32.5 nTPM
Esophagus	32.5 nTPM

Undifferentiated cells	5.8 nTPM
Rectum	9.2 nTPM
Small intestine	6.7 nTPM
Colon	1.3 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC