Single cell type - FAM174B - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	FAM174B
Gene descriptionⁱ Full gene name according to HGNC.	Family with sequence similarity 174 member B
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Membrane
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	8

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Mucus-secreting cells - Mucin production (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Alveolar cells type 1, Intestinal goblet cells, Late spermatids)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Immune cell enhanced (T-reg)
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Detected in many
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (CACO-2, MCF7, OE19, RPMI-8226, RT4)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Alveolar cells type 1, Intestinal goblet cells, Late spermatids)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

FAM174B is part of cluster 27 Mucus-secreting cells - Mucin production with confidenceⁱ 1
121 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
RASEF	RAS and EF-hand domain containing	0.5573	35
MLPH	Melanophilin	0.5551	27
TMCO3	Transmembrane and coiled-coil domains 3	0.5470	27
HTR3C	5-hydroxytryptamine receptor 3C	0.5422	31
MYO5C	Myosin VC	0.5363	11
CAPN9	Calpain 9	0.5297	27
SPDEF	SAM pointed domain containing ETS transcription factor	0.5210	27
GALNT8	Polypeptide N-acetylgalactosaminyltransferase 8	0.5128	31
ANO7	Anoctamin 7	0.5000	27
CREB3L4	CAMP responsive element binding protein 3 like 4	0.4908	35
KLK15	Kallikrein related peptidase 15	0.4905	27
NEURL1	Neuralized E3 ubiquitin protein ligase 1	0.4845	25
TTC39A	Tetratricopeptide repeat domain 39A	0.4823	34
BCAS1	Brain enriched myelin associated protein 1	0.4811	27
B3GNT6	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 6	0.4811	27

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	8.5 nTPM
Adipose tissue	8.8 nTPM
Breast	8.2 nTPM

Alveolar cells type 2	22.2 nTPM
Lung	22.2 nTPM

Astrocytes	13.7 nTPM
Brain	13.7 nTPM

B-cells	1.7 nTPM
Liver	6.6 nTPM
Breast	6.6 nTPM
Bone marrow	1.7 nTPM
Spleen	0.7 nTPM
Lymph node	0.6 nTPM
Stomach	0.5 nTPM
Colon	0.0 nTPM
Kidney	0.0 nTPM
PBMC	0.0 nTPM
Adipose tissue	0.0 nTPM

Basal keratinocytes	2.3 nTPM
Skin	2.3 nTPM

Dendritic cells	2.3 nTPM
PBMC	4.0 nTPM
Breast	1.5 nTPM
Adipose tissue	1.3 nTPM

Endothelial cells	10.3 nTPM
Lung	34.3 nTPM
Ovary	21.0 nTPM
Pancreas	19.3 nTPM
Heart muscle	14.4 nTPM
Breast	13.3 nTPM
Skin	11.5 nTPM
Testis	8.0 nTPM
Esophagus	6.4 nTPM
Placenta	6.2 nTPM
Skeletal muscle	5.7 nTPM
Endometrium	2.9 nTPM
Prostate	2.0 nTPM
Eye	0.0 nTPM
Liver	0.0 nTPM

Fibroblasts	4.9 nTPM
Skin	12.3 nTPM
Stomach	7.4 nTPM
Esophagus	6.5 nTPM
Skeletal muscle	4.6 nTPM
Breast	4.6 nTPM
Lung	4.5 nTPM
Adipose tissue	4.4 nTPM
Prostate	3.8 nTPM
Heart muscle	2.9 nTPM
Ovary	1.7 nTPM
Placenta	1.3 nTPM

Intestinal goblet cells	60.9 nTPM
Colon	72.2 nTPM
Rectum	57.4 nTPM
Small intestine	53.0 nTPM

Macrophages	4.9 nTPM
Ovary	40.3 nTPM
Esophagus	6.3 nTPM
Lung	4.6 nTPM
Adipose tissue	3.5 nTPM
Endometrium	3.4 nTPM
Breast	2.7 nTPM
Bone marrow	2.5 nTPM
Kidney	2.3 nTPM
Prostate	2.2 nTPM
Skeletal muscle	2.1 nTPM
Spleen	2.1 nTPM
Testis	1.7 nTPM
PBMC	0.0 nTPM
Pancreas	0.0 nTPM
Stomach	0.0 nTPM

Paneth cells	1.6 nTPM
Rectum	4.8 nTPM
Colon	0.0 nTPM
Small intestine	0.0 nTPM

Plasma cells	6.6 nTPM
Esophagus	12.3 nTPM
Adipose tissue	11.6 nTPM
Bone marrow	3.3 nTPM
Stomach	3.3 nTPM
Spleen	2.3 nTPM

Respiratory ciliated cells	14.5 nTPM
Lung	16.0 nTPM
Bronchus	13.0 nTPM

Smooth muscle cells	3.7 nTPM
Prostate	8.4 nTPM
Skin	6.6 nTPM
Pancreas	6.1 nTPM
Endometrium	4.8 nTPM
Esophagus	4.6 nTPM
Bronchus	2.9 nTPM
Breast	2.4 nTPM
Adipose tissue	2.1 nTPM
Skeletal muscle	1.4 nTPM
Ovary	1.3 nTPM
Heart muscle	0.6 nTPM

T-cells	3.4 nTPM
Prostate	23.6 nTPM
Endometrium	5.2 nTPM
Colon	4.3 nTPM
Stomach	4.3 nTPM
Lymph node	3.2 nTPM
Lung	3.0 nTPM
Skin	2.3 nTPM
Breast	2.2 nTPM
PBMC	2.0 nTPM
Bone marrow	1.9 nTPM
Spleen	1.4 nTPM
Adipose tissue	1.2 nTPM
Skeletal muscle	1.0 nTPM
Liver	0.9 nTPM
Kidney	0.7 nTPM
Ovary	0.0 nTPM
Esophagus	0.0 nTPM

Collecting duct cells	7.3 nTPM
Kidney	7.3 nTPM

Distal tubular cells	7.9 nTPM
Kidney	7.9 nTPM

Early spermatids	22.6 nTPM
Testis	22.6 nTPM

Endometrial stromal cells	4.2 nTPM
Endometrium	4.2 nTPM

Undifferentiated cells	5.5 nTPM
Rectum	6.0 nTPM
Colon	5.6 nTPM
Small intestine	4.9 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC