Single cell type - AGRN - The Human Protein Atlas

GENERAL INFORMATIONⁱ General description of the gene and the encoded protein(s) using information from HGNC and Ensembl, as well as predictions made by the Human Protein Atlas project.
Gene nameⁱ Official gene symbol, which is typically a short form of the gene name, according to HGNC.	AGRN
Gene descriptionⁱ Full gene name according to HGNC.	Agrin
Protein classⁱ Assigned HPA protein class(es) for the encoded protein(s). Read more	Disease related genes Human disease related genes Plasma proteins Potential drug targets Transporters
Predicted locationⁱ All transcripts of all genes have been analyzed regarding the location(s) of corresponding protein based on prediction methods for signal peptides and transmembrane regions. Genes with at least one transcript predicted to encode a secreted protein, according to prediction methods or to UniProt location data, have been further annotated and classified with the aim to determine if the corresponding protein(s) are secreted or actually retained in intracellular locations or membrane-attached. Remaining genes, with no transcript predicted to encode a secreted protein, will be assigned the prediction-based location(s). The annotated location overrules the predicted location, so that a gene encoding a predicted secreted protein that has been annotated as intracellular will have intracellular as the final location. Read more	Intracellular,Secreted
Number of transcriptsⁱ Number of protein-coding transcripts from the gene as defined by Ensembl.	4

HUMAN PROTEIN ATLAS INFORMATIONⁱ Summary of RNA expression and protein localization based on data generated within the Human Protein Atlas project.
Single cell type expression clusterⁱ The RNA data was used to cluster genes according to their expression across single cell types. Clusters contain genes that have similar expression patterns, and each cluster has been manually annotated to describe common features in terms of function and specificity. Read more	Respiratory epithelial cells - Mucosal defense (mainly)
Single cell type specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed cell types based on scRNA-seq data from normal tissues. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Cell type enhanced (Collecting duct cells, Basal respiratory cells, Ductal cells, Pancreatic endocrine cells)
Immune cell specificityⁱ The RNA specificity category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: cell type enriched, group enriched, cell type enhanced, low cell type specificity and not detected. Read more	Not detected in immune cells
Immune cell distributionⁱ The RNA distribution category is based on mRNA expression levels in the analyzed samples based on data from HPA. The categories include: detected in all, detected in many, detected in some, detected in single and not detected. Read more	Not detected
Cell line specificityⁱ RNA specificity category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into six different categories (enriched, group enriched, enhanced, low specificity and not detected) according to their RNA expression levels across the panel of cell lines. Read more	Cell line enhanced (CAPAN-2, EFO-21, HaCaT)
Cell line distributionⁱ RNA distribution category based on RNA sequencing data from all cell lines in the Human Protein Atlas. Genes are classified into five different categories (detected in all, detected in many, detected in some, detected in single and not detected) according to their pattern of detected RNA expression across the panel of cell lines. Read more	Detected in many
Protein evidenceⁱ Evidence score for genes based on UniProt protein existence (UniProt evidence); a Human Protein Atlas antibody- or RNA based score (HPA evidence); and evidence based on PeptideAtlas (MS evidence). The avaliable scores are evidence at protein level, evidence at transcript level, no evidence, or not avaliable. Read more	Evidence at protein level
Extracellular locationⁱ A possible secretion of the protein was predicted based on N-terminal signal sequence (signal peptide) predictions and transmembrane region predictions, and subsequently annotated for its local or systemic secretion based on literature, function, and protein and RNA expression data. Read more	Protein predicted to be secreted

SINGLE CELL TYPESⁱ A summary of single cell RNA (nTPM) from all single cell types. Color-coding is based on cell type groups, each consisting of cell types with functional features in common.
Single cell types Group Expression Alphabetical RNA single cell type specificity: Cell type enhanced (Collecting duct cells, Basal respiratory cells, Ductal cells, Pancreatic endocrine cells)

SINGLE CELL TISSUES OVERVIEWⁱ An overview of all tissues where single cell type expression has been analyzed. Each tissue name and corresponding UMAP plot is clickable and redirects to the selected tissue.
Adipose tissue Bone marrow Brain Breast Bronchus Colon Endometrium Esophagus Eye Heart muscle Kidney Liver Lung Lymph node Ovary Pancreas Placenta Prostate Rectum Skeletal muscle Skin Small intestine Spleen Stomach Testis

EXPRESSION CLUSTERING & CORRELATIONⁱ

AGRN is part of cluster 26 Respiratory epithelial cells - Mucosal defense with confidenceⁱ 1
293 genes in cluster

Go to interactive expression cluster page

15 nearest neighbours based on single cell type RNA expression

Neighbourⁱ Gene name according to HGNC.	Descriptionⁱ Gene description according to HGNC.	Correlationⁱ Correlation between the selected gene and neighboring gene. Correlation is calculated as Spearman correlation in PCA space based on the RNA-seq expression data. Read more	Clusterⁱ ID of the expression cluster of the neighboring gene. Read more
CCND1	Cyclin D1	0.7284	1
CELSR1	Cadherin EGF LAG seven-pass G-type receptor 1	0.6483	44
AMTN	Amelotin	0.6455	26
KRTAP2-3	Keratin associated protein 2-3	0.6355	1
RNF183	Ring finger protein 183	0.6244	44
IGFL1	IGF like family member 1	0.6070	26
KRT81	Keratin 81	0.6064	1
S100A7	S100 calcium binding protein A7	0.6018	26
CHST4	Carbohydrate sulfotransferase 4	0.5956	67
TMEM211	Transmembrane protein 211	0.5901	26
VANGL1	VANGL planar cell polarity protein 1	0.5884	26
SLC4A11	Solute carrier family 4 member 11	0.5814	26
CCDC9B	Coiled-coil domain containing 9B	0.5789	1
FZD6	Frizzled class receptor 6	0.5747	44
SLC28A3	Solute carrier family 28 member 3	0.5726	26

SINGLE CELL TISSUESⁱ Single cell RNA levels of annotated cell type clusters are shown separately for each analyzed tissue. For easier navigation across tissues, use the above Single cell tissue overview, where each tissue name and UMAP plot is clickable and redirects to the selected tissue, alternatively the grey browser on the left side of the window. Barchart shows nTPM levels in each annotated cluster of single cells. To the left, a UMAP plot illustrates the RNA expression profile. The tissue name and plots are clickable and redirect to a more detailed interactive page. Color-coding is based on cell type groups, each consisting of cell types with functional features in common. Please note that the same cell type may be found in several clusters.
Cluster Expression Alphabetical Adipose tissue
Cluster Expression Alphabetical Bone marrow
Cluster Expression Alphabetical Brain
Cluster Expression Alphabetical Breast
Cluster Expression Alphabetical Bronchus
Cluster Expression Alphabetical Colon
Cluster Expression Alphabetical Endometrium
Cluster Expression Alphabetical Esophagus
Cluster Expression Alphabetical Eye
Cluster Expression Alphabetical Heart muscle
Cluster Expression Alphabetical Kidney
Cluster Expression Alphabetical Liver
Cluster Expression Alphabetical Lung
Cluster Expression Alphabetical Lymph node
Cluster Expression Alphabetical Ovary
Cluster Expression Alphabetical Pancreas
Cluster Expression Alphabetical Placenta
Cluster Expression Alphabetical Prostate
Cluster Expression Alphabetical Rectum
Cluster Expression Alphabetical Skeletal muscle
Cluster Expression Alphabetical Skin
Cluster Expression Alphabetical Small intestine
Cluster Expression Alphabetical Spleen
Cluster Expression Alphabetical Stomach
Cluster Expression Alphabetical Testis

BLOODⁱ

Single cell data

Cluster

Expression

Alphabetical

PBMC

Flow sorted data

Lineage

Expression

Alphabetical

CELL LINESⁱ

Organ

Expression

Alphabetical

Adipocytes	27.4 nTPM
Adipose tissue	28.6 nTPM
Breast	26.2 nTPM

Alveolar cells type 1	25.6 nTPM
Lung	25.6 nTPM

Alveolar cells type 2	8.8 nTPM
Lung	8.8 nTPM

Astrocytes	15.0 nTPM
Brain	15.0 nTPM

B-cells	2.2 nTPM
Kidney	9.5 nTPM
Breast	4.0 nTPM
Colon	3.2 nTPM
Stomach	1.4 nTPM
Liver	1.3 nTPM
Lymph node	1.1 nTPM
Bone marrow	0.8 nTPM
Adipose tissue	0.5 nTPM
Spleen	0.3 nTPM
PBMC	0.0 nTPM

Dendritic cells	0.9 nTPM
Breast	1.8 nTPM
Adipose tissue	0.8 nTPM
PBMC	0.0 nTPM

Endothelial cells	29.0 nTPM
Ovary	136.0 nTPM
Endometrium	41.3 nTPM
Eye	32.4 nTPM
Breast	28.3 nTPM
Skeletal muscle	28.2 nTPM
Placenta	26.1 nTPM
Esophagus	24.4 nTPM
Testis	21.0 nTPM
Pancreas	19.3 nTPM
Liver	14.7 nTPM
Skin	14.4 nTPM
Prostate	12.0 nTPM
Lung	6.2 nTPM
Heart muscle	1.5 nTPM

Fibroblasts	8.7 nTPM
Lung	26.8 nTPM
Stomach	20.8 nTPM
Ovary	11.0 nTPM
Placenta	8.3 nTPM
Breast	6.8 nTPM
Skeletal muscle	5.8 nTPM
Skin	4.8 nTPM
Esophagus	4.2 nTPM
Prostate	3.4 nTPM
Adipose tissue	3.1 nTPM
Heart muscle	0.6 nTPM

Intestinal goblet cells	11.6 nTPM
Rectum	15.6 nTPM
Colon	10.0 nTPM
Small intestine	9.2 nTPM

Macrophages	4.3 nTPM
Endometrium	10.6 nTPM
Pancreas	10.2 nTPM
Ovary	8.7 nTPM
Breast	7.5 nTPM
Skeletal muscle	5.0 nTPM
Adipose tissue	4.3 nTPM
Lung	4.1 nTPM
Prostate	3.4 nTPM
Esophagus	3.1 nTPM
Kidney	2.3 nTPM
Spleen	2.1 nTPM
Testis	1.7 nTPM
Bone marrow	1.2 nTPM
PBMC	0.0 nTPM
Stomach	0.0 nTPM

Paneth cells	17.7 nTPM
Rectum	25.0 nTPM
Small intestine	15.0 nTPM
Colon	13.2 nTPM

Plasma cells	0.6 nTPM
Bone marrow	1.7 nTPM
Stomach	0.9 nTPM
Adipose tissue	0.6 nTPM
Esophagus	0.0 nTPM
Spleen	0.0 nTPM

Respiratory ciliated cells	33.0 nTPM
Bronchus	60.2 nTPM
Lung	5.8 nTPM

Smooth muscle cells	23.3 nTPM
Ovary	72.3 nTPM
Endometrium	58.2 nTPM
Adipose tissue	29.0 nTPM
Bronchus	20.8 nTPM
Breast	18.2 nTPM
Skin	15.7 nTPM
Skeletal muscle	15.5 nTPM
Pancreas	15.4 nTPM
Prostate	5.3 nTPM
Esophagus	4.6 nTPM
Heart muscle	1.0 nTPM

T-cells	2.9 nTPM
Esophagus	13.1 nTPM
Endometrium	8.0 nTPM
Skin	7.8 nTPM
Lung	4.5 nTPM
Breast	4.5 nTPM
Colon	3.3 nTPM
Skeletal muscle	3.0 nTPM
Stomach	1.5 nTPM
Kidney	1.4 nTPM
Lymph node	0.7 nTPM
Adipose tissue	0.5 nTPM
Spleen	0.5 nTPM
Liver	0.4 nTPM
PBMC	0.2 nTPM
Bone marrow	0.1 nTPM
Prostate	0.0 nTPM
Ovary	0.0 nTPM

Cholangiocytes	61.5 nTPM
Liver	61.5 nTPM

Cone photoreceptor cells	8.4 nTPM
Eye	8.4 nTPM

Cytotrophoblasts	1.6 nTPM
Placenta	1.6 nTPM

Distal enterocytes	29.9 nTPM
Rectum	37.5 nTPM
Colon	22.3 nTPM

Undifferentiated cells	18.3 nTPM
Rectum	25.1 nTPM
Colon	21.5 nTPM
Small intestine	8.2 nTPM

Adipose tissue

Bone marrow

Brain

Breast

Bronchus

Colon

Endometrium

Esophagus

Eye

Heart muscle

Kidney

Liver

Lung

Lymph node

Ovary

Pancreas

Placenta

Prostate

Rectum

Skeletal muscle

Skin

Small intestine

Spleen

Stomach

Testis

PBMC